Search results for " biodegradation"
showing 6 items of 46 documents
Genome Sequence of Rhodococcus sp. Strain BCP1, a Biodegrader of Alkanes and Chlorinated Compounds
2013
Rhodococcus sp. strain BCP1 (DSM 44980) co-metabolizes chlorinated compounds and mineralizes a broad range of alkanes being highly tolerant to these toxic chemicals. Here, we present the high-quality draft genome sequence of strain BCP1 consisting of 6,231,823 bp, with a G+C content of 70.4%, 5,902 protein-coding genes, and 58 RNAs genes. Rhodococcus genus comprises Gram-positive, non-sporulating, aerobic bacteria that are widely distributed in the environment (1). Rhodococcus sp. strain BCP1 (formerly: Rhodococcus aetherovorans strain BCP1, DSM 44980) was selected from an aerobic butane-utilizing consortium as the prevailing isolate able to co-metabolize chloroform, vinyl chloride and tric…
Variability of pesticide mineralization in individual soil aggregates of millimeter size
2002
The aim of this study was to compare the 2,4-D mineralization in individual aggregates of millimeter size (3 size classes and 96 aggregates per size class). 14 CO 2 coming from the mineralization of 14 C ring-labeled 2,4-D and evolved by each aggregate incubated in microtiter plates was trapped with barium hydroxide on filters and measured using a Phosphorimager. We observed an important variability of mineralization in aggregates of each size class and in the different size classes of aggregates. The main factors responsible for these fluctuations could be a heterogeneous distribution of degrading microorganisms or of available carbon necessary for cometabolism.
Soil aggregates and humus systems
2016
The European Society for Soil Conservation Conference, organized by the Babes-Bolyai University will take place from 15th June to the 18th June 2016 at the Babes-Bolyai University of Cluj-Napoca in Cluj-Napoca, Romania. The conference will cover areas like Soils – Our Common Future” stresses the vital interconnection between soil and life, as well as its importance for the future of the human society.; International audience; The survey of few main morphological soil aggregates (with intrinsic biological, chemical and physical contents) reveals the existence of different humus systems. Recognizable by naked eyes in the field, each humus system is confined in an ecological frame (climate, ve…
Dispersal potential of antibiotrophy along the manure-soil-sediment continuum of sulfamethazine-contaminated agrosystems
2021
An emerging function in the scientific literature, antibiotrophy is the bacterial capacity to metabolize antibiotics. Considered a bioremediation tool, it remains a health risk due to its association with antibiotic resistance. The first goal of this thesis was to study its dispersion within agrosystems contaminated with antibiotics during two coalescence events, i.e. manure spreading on the soil and its erosion towards the aquatic compartment. Experiments have shown the systematic invasion of the soil and sediment bacterial communities by the antibiotroph Microbacterium sp. C448 when sulfamethazine is present but not necessarily mineralized. The antibiotic would have a double function towa…
Cloning of an alkane hydroxylase system in a long chain n-alkane- degrader Gordonia sp.
2008
Five Gram-positive GC rich n-alkane degraders were isolated from a long-term accidentally contaminated beach in Sicily and identified as one Nocardia, two Rhodococcus and two Gordonia strains (Quatrini et al., 2008 J. Appl. Microbiol. 104:251-9). All the isolates were able to grow on long and very long chain n-alkanes up to C36. Diverging alkane-hydroxylase encoding genes (alkB) were detected by PCR using degenerated primers in all the strains. Multiple sequences were obtained from the Nocardia strain while only one alkB gene was detected in Rhodococcus and Gordonia. The aim of this work is to genetically characterize the alk cluster in one of the two Gordonia strains called SoCg. Pulsed Fi…
Tuning of biodegradation rate of PLLA scaffolds via blending with PLA
2009
Blends of Poly-L-Lactic Acid (PLLA) with Poly-Lactic Acid (PLA) in different proportions (95/5, 90/10, 80/20, 70/30 and 60/40) were utilized in order to realize biodegradable and biocompatible scaffolds for soft tissue engineering applications. The scaffolds were produced via thermally induced phase separation (TIPS) starting from ternary systems where dioxane was the solvent and water the non-solvent. Morphology was evaluated by Scanning Electron Microscopy (average pore size and interconnection). Foams’ apparent density was also evaluated (porosity ranges from 87% to 92%). Moreover an in vitro biodegradation test of scaffolds was set-up in order to verify the rate of degradation of the va…