Search results for " biotechnology"
showing 10 items of 3723 documents
Modelling the removal and reversible immobilization of murine noroviruses in a Phaeozem under various contamination and rinsing conditions
2018
International audience; Enteric viruses entering the soil with contaminated irrigation water can reach groundwater or be internalized in plants through their roots without being inactivated. Their fate in the soil depends on the virus, the soil and the soil solution. In order to write a mathematical model suitable for a Calcaric Phaeozem, we investigated the removal of murine norovirus and reversible immobilization in aggregate columns according to a saturation procedure, conditions between contamination and rinsing time, temperature and soil solution. Viruses were quantified before and after 0.45‐μm filtration with an RT‐qPCR (real‐time polymerase chain reaction). Experimental results supp…
Peptide-mediated interference with baculovirus transduction
2007
Baculovirus represents a multifunctional platform with potential for biomedical applications including disease therapies. The importance of F3, a tumor-homing peptide, in baculovirus transduction was previously recognized by the ability of F3 to augment viral binding and gene delivery to human cancer cells following display on the viral envelope. Here, F3 was utilized as a molecular tool to expand understanding of the poorly characterized baculovirus-mammalian cell interactions. Baculovirus-mediated transduction of HepG2 hepatocarcinoma cells was strongly inhibited by coincubating the virus with synthetic F3 or following incorporation of F3 into viral nucleocapsid by genetic engineering, th…
Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.
2008
To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…
Internalization of novel non-viral vector TAT-streptavidin into human cells
2007
BMC Biotechnology, 7 (1)
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…
Molecular Characterization of a Variant of Bacillus anthracis-Specific Phage AP50 with Improved Bacteriolytic Activity▿ †
2008
ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.
H&E Multi-Laboratory Staining Variance Exploration with Machine Learning
2022
In diagnostic histopathology, hematoxylin and eosin (H&E) staining is a critical process that highlights salient histological features. Staining results vary between laboratories regardless of the histopathological task, although the method does not change. This variance can impair the accuracy of algorithms and histopathologists’ time-to-insight. Investigating this variance can help calibrate stain normalization tasks to reverse this negative potential. With machine learning, this study evaluated the staining variance between different laboratories on three tissue types. We received H&E-stained slides from 66 different laboratories. Each slide contained kidney, skin, and colon tissue sampl…
Particle balance and return loops for microplastics in a tertiary-level wastewater treatment plant.
2021
Highlights: Dewatering by centrifugation was a step that removed a high number of MPs from the sludge. Sludge retained especially the fibrous microplactics. Reject water transported microplastics inside a wastewater treatment plant. Disc filter-based tertiary treatment ensured removal of 99% of microplastics in wastewater. Microplastics (MPs) from households, stormwater, and various industries are transported to wastewater treatment plants (WWTPs), where a high proportion of them are captured before discharging their residuals to watersheds. Although recent studies have indicated that the removed MPs are mainly retained in wastewater sludge, sludge treatment processes have gained less atten…
Alternative analysis of transient infiltration experiment to estimate soil water repellency
2019
The repellency index (RI) defined as the adjusted ratio between soil‐ethanol, Se, and soil‐water, Sw, sorptivities estimated from minidisk infiltrometer experiments has been used instead of the widely used water drop penetration time and molarity of ethanol drop tests to assess soil water repellency. However, sorptivity calculated by the usual early‐time infiltration equation may be overestimated as the effects of gravity and lateral capillary are neglected. With the aim to establish the best applicative procedure to assess RI, different approaches to estimate Se and Sw were compared that make use of both the early‐time infiltration equation (namely, the 1 min, S1, and the short‐time linear…
Ginger and Turmeric Essential Oils for Weed Control and Food Crop Protection
2019
Ginger and turmeric are two food ingredients that are in high demand due to their flavor and positive effects on health. The biological properties of these spices are closely related to the aromatic compounds they contain. The chemical compositions of their essential oils and their in vitro phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, Echinochloa crus-galli, Cortaderia selloana, and Nicotiana glauca) and food crops (tomato, cucumber, and rice) were studied. Forty-one compounds, accounting for a relative peak area of 87.7% and 94.6% of turmeric and ginger essential oils, respectively, were identified by Gas Chromatography&ndash