Search results for " colon"
showing 10 items of 718 documents
p63 Isoforms Regulate Metabolism of Cancer Stem Cells
2014
p63 is an important regulator of epithelial development expressed in different variants containing (TA) or lacking (ΔN) the N-terminal transactivation domain. The different isoforms regulate stem-cell renewal and differentiation as well as cell senescence. Several studies indicate that p63 isoforms also play a role in cancer development; however, very little is known about the role played by p63 in regulating the cancer stem phenotype. Here we investigate the cellular signals regulated by TAp63 and ΔNp63 in a model of epithelial cancer stem cells. To this end, we used colon cancer stem cells, overexpressing either TAp63 or ΔNp63 isoforms, to carry out a proteomic study by chemical-labeling …
Lentivirus-induced dendritic cells for immunization against high-risk WT1(+) acute myeloid leukemia.
2013
Wilms' tumor 1 antigen (WT1) is overexpressed in acute myeloid leukemia (AML), a high-risk neoplasm warranting development of novel immunotherapeutic approaches. Unfortunately, clinical immunotherapeutic use of WT1 peptides against AML has been inconclusive. With the rationale of stimulating multiantigenic responses against WT1, we genetically programmed long-lasting dendritic cells capable of producing and processing endogenous WT1 epitopes. A tricistronic lentiviral vector co-expressing a truncated form of WT1 (lacking the DNA-binding domain), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-4 (IL-4) was used to transduce human monocytes ex vivo. Overnight transd…
No inbreeding depression but increased sexual investment in highly inbred ant colonies.
2012
Inbreeding can lead to the expression of deleterious recessive alleles and to a subsequent fitness reduction. In Hymenoptera, deleterious alleles are purged in haploid males moderating inbreeding costs. However, in these haplodiploid species, inbreeding can result in the production of sterile diploid males. We investigated the effects of inbreeding on the individual and colony level in field colonies of the highly inbred ant Hypoponera opacior. In this species, outbreeding winged sexuals and nest-mating wingless sexuals mate during two separate reproductive periods. We show that regular sib-matings lead to high levels of homozygosity and the occasional production of diploid males, which spo…
Colonización territorial, razón ilustrada y particularidades valencianas en la nueva población de Benadressa (Castelló de la Plana)
2008
El presente artículo analiza la proyección de una nueva población en el interior de la Plana de Castelló (N. del País Valenciano), un proceso de colonización territorial realizado al amparo de la reposición del denominado Fuero o Privilegio Alfonsino (segunda mitad del s.XVIII). El ejemplo de Benadressa permite comprender las complejas relaciones de poder e ideología que se dieron en este tipo de planes, de iniciativa privada pero insertados plenamente en el proyecto económico y territorial de la Ilustración. A su vez, se trata de un capítulo más de las profundas transformaciones sufridas por la Plana de Castelló en la Edad Moderna, principalmente basadas en el avance de la superficie culti…
Porta Nuova a Palermo (XVI secolo). La vicenda, i protagonisti , i modelli, in Entrare in città: di archi e di porte, numero monografico della rivist…
2015
La Porta Nuova a Palermo, realizzata nel corso della seconda metà del Cinquecento, costituisce un episodio singolare per le molteplici valenze funzionali, simboliche e celebrative e per il carattere eccezionale della sua figurazione legata alla retorica trionfalistica di Carlo V. I differenti cantieri che si sono susseguiti delineano una vicenda complessa e originale, strettamente connessa con quelle della via Toledo e del Palazzo Reale (al quale la porta è collegata), e ricca di personalità e apporti esterni. Il ruolo dell’ingegnere regio Antonio Salamone coinvolto in tale vicenda e le analogie con talune soluzioni adottate in altre realizzazioni del tempo possono contribuire a ridefinire …
Girolamo I Colonna e l’arredo d’altare in corallo della Madonna del Buon Consiglio di Genazzano
2020
Si delinea brevemente la figura di Girolamo I Colonna, di Filippo I, principe di Paliano, e Anna Tomacelli. Il cardinale romano, uno dei primi raffinati collezionisti di casa Colonna, che predilesse la pittura di importanti maestri bolognesi e dei loro allievi, nel 1639-1640 si rivolgeva ad abili maestri del barocco siciliano per la realizzazione di un prezioso arredo d’altare in rame e corallo composto da sei candelieri e da una croce. L’inedita corrispondenza tra Girolamo I e il fratello Marcantonio V, punto di riferimento in Sicilia in quel periodo per i Colonna per aver sposato la ricca ereditiera siciliana Isabella Gioeni, e tramite per la realizzazione dei manufatti, riporta interessa…
Una moderna sensibilità romantica: la poesia di Giuseppina Turrisi Colonna
2015
Attraverso un excursus tematico dei principali nuclei poetici, il saggio vuole dimostrare come anche per l’opera della Turrisi Colonna, cronologicamente assai breve e geograficamente assai distante dal dibattito intellettuale romantico, italiano ed europeo, allora in corso, sia possibile parlare di modernità letteraria e riscontrare, nella colta sensibilità femminile della giovane poetessa siciliana, tracce di un acceso fermento nazionalistico tutto italiano.
Global distributions of diazotrophs abundance and biomass - Depth integrated values computed from a collection of source datasets - Contribution to t…
2013
The MAREDAT atlas covers 11 types of plankton, ranging in size from bacteria to jellyfish. Together, these plankton groups determine the health and productivity of the global ocean and play a vital role in the global carbon cycle. Working within a uniform and consistent spatial and depth grid (map) of the global ocean, the researchers compiled thousands and tens of thousands of data points to identify regions of plankton abundance and scarcity as well as areas of data abundance and scarcity. At many of the grid points, the MAREDAT team accomplished the difficult conversion from abundance (numbers of organisms) to biomass (carbon mass of organisms). The MAREDAT atlas provides an unprecedente…
The designer cytokine hyper-IL-6 mediates growth inhibition and GM-CSF-dependent rejection of B16 melanoma cells.
2000
The low immunogenic B16 melanoma cell line was transfected with a mammalian expression vector containing the complementary DNA for a sIL-6R/IL-6 fusion protein, termed Hyper-IL-6 (H-IL-6), which was shown to have biological activities at 100-1000-fold lower concentrations than IL-6 in combination with sIL-6R. The secreted p84 glycoprotein was detected in the supernatant of transfected cells and was fully active on BAF3/gp130 cells, which respond to IL-6/sIL-6R but not to IL-6 alone. Administration of recombinant H-IL-6 to C57BL/6 mice resulted in a prolonged acute phase protein gene expression indicating long systemic persistence of the fusion protein. Transfected B16 cells (B16/H-IL6 cells…
GM-CSF restores innate, but not adaptive, immune responses in glucocorticoid-immunosuppressed human blood in vitro.
2003
Abstract Infection remains the major complication of immunosuppressive therapy in organ transplantation. Therefore, reconstitution of the innate immunity against infections, without activation of the adaptive immune responses, to prevent graft rejection is a clinically desirable status in transplant recipients. We found that GM-CSF restored TNF mRNA and protein expression without inducing IL-2 production and T cell proliferation in glucocorticoid-immunosuppressed blood from either healthy donors or liver transplant patients. Gene array experiments indicated that GM-CSF selectively restored a variety of dexamethasone-suppressed, LPS-inducible genes relevant for innate immunity. A possible ex…