Search results for " culture"
showing 10 items of 5779 documents
Optical characteristics of greenhouse plastic films affect yield and some quality traits of spinach (Spinacia oleracea L.) subjected to different nit…
2021
Light and nitrogen strongly affect the growth, yield, and quality of food crops, with greater importance in green leafy vegetables for their tendency to accumulate nitrate in leaves. The purpose of this research was to explore the effect of two greenhouse films (Film A and B) on yield, and quality of spinach grown under different nitrogen regimes (not fertilized—N0%
Cytological and molecular characterization of three gametoclones of Citrus clementina
2013
Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (array-CGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a sing…
Cherry Tomato Drying: Sun versus Convective Oven
2021
Solar drying and convective oven drying of cherry tomatoes (Solanum lycopersicum) were compared. The changes in the chemical parameters of tomatoes and principal drying parameters were recorded during the drying process. Drying curves were fitted to several mathematical models, and the effects of air temperature during drying were evaluated by multiple regression analyses, comparing to previously reported models. Models for drying conditions indicated a final water content of 30% (semidry products) and 15% (dry products) was achieved, comparing sun-drying and convective oven drying at three different temperatures. After 26–28 h of sun drying, the tomato tissue had reached a moisture content…
Subcellular localization and purification of a p-hydroxyphenylpyruvate dioxygenase from cultured carrot cells and characterization of the correspondi…
1997
p-Hydroxyphenylpyruvate dioxygenase catalyses the transformation of p-hydroxyphenylpyruvate into homogentisate. In plants this enzyme has a crucial role because homogentisate is the aromatic precursor of all prenylquinones. Furthermore this enzyme was recently identified as the molecular target for new families of potent herbicides. In this study we examine precisely the localization of p-hydroxyphenylpyruvate dioxygenase activity within carrot cells. Our results provide evidence that, in cultured carrot cells, p-hydroxyphenylpyruvate dioxygenase is associated with the cytosol. Purification and SDS/PAGE analysis of this enzyme revealed that its activity is associated with a polypeptide of 4…
New national and regional Annex I Habitat records: from #21 to #25
2021
New Italian data on the distribution of the Annex I Habitats 3170*, 6110*, 91E0*, 9320, 9330 are reported in this contribution. Specifically, one new occurrence in Natura 2000 sites is presented and six new cells are added in the European Environment Agency 10 km × 10 km reference grid. The new data refer to the Italian administrative regions of Sardinia, Sicily and Umbria.
Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study
2016
Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Eh…
Recovery of polyhydroxyalkanoates (PHAs) from wastewater : a review
2020
Abstract Polyhydroxyalkanoates (PHAs) are biopolyesters accumulated as carbon and energy storage materials under unbalanced growth conditions by various microorganisms. They are one of the most promising potential substitutes for conventional non-biodegradable plastics due to their similar physicochemical properties, but most important, its biodegradability. Production cost of PHAs is still a great barrier to extend its application at industrial scale. In order to reduce that cost, research is focusing on the use of several wastes as feedstock (such as agro-industrial and municipal organic waste and wastewater) in a platform based on mixed microbial cultures. This review provides a critical…
An Updated Checklist of the Sicilian Native Edible Plants: Preserving the Traditional Ecological Knowledge of Century-Old Agro-Pastoral Landscapes
2020
The traditional use of native wild food plants (NWFP) may represent a valuable supplementary food source for the present and future generations. In Sicily, the use of wild plants in the human diet dates back to very ancient times and still plays an important role in some rural communities. Moreover, in this regard, the natural and cultural inheritance of this island is wealthy and diversified for several reasons. First, Sicily hosts a rich vascular flora, with 3,000 native and 350 endemic plants. Second, due to its central position in the Mediterranean, the island has acted as a veritable melting pot for the ethnobotanical knowledge of the rural communities of the entire basin. We reviewed …
Colonization of flax roots and early physiological responses of flax cells inoculated with pathogenic and nonpathogenic strains of Fusarium oxysporum
2003
ABSTRACT Fusarium oxysporum includes nonpathogenic strains and pathogenic strains that can induce necrosis or tracheomycosis in plants. The objective of this study was to compare the abilities of a pathogenic strain (Foln3) and a nonpathogenic strain (Fo47) to colonize flax roots and to induce early physiological responses in flax cell culture suspensions. Both strains colonized the outer cortex of the root; however, plant defense reactions, i.e., the presence of wall appositions, osmiophilic material, and collapsed cells, were less frequent and less intense in a root colonized by Foln3 than by Fo47. Early physiological responses were measured in flax cell suspensions confronted with germin…
Genetic Transformation of Serratula tinctoria (Dyer’s Savory) for Ecdysteroid Production
1999
Serratula tinctoria is a perennial plant of the Compositae family with medium-sized, serrated leaves and purple flowers (Loste 1937). This plant, also known as dyer’s savory, is widespread in Europe but with an irregular distribution. Inflorescences (capitula) are purple and are usually unisexual, staminate, or pistillate. In Europe, the flowering period extends from July to September. More than 40 species have been described in Europe, North Africa, and Asia. The plants produce large amounts of secondary metabolites, in particular ecdysteroids at very high concentration in roots (up to 2% dry wt.), in flowers, and in leaves (Bathori et al. 1986; Rudel et al. 1992; Corio-Costet et al. 1993b…