Search results for " cultures"
showing 10 items of 168 documents
Climatic gradients along the windward slopes of Mount Kenya and their implication for crop risks. Part 2 : crop sensitivity.
2016
16 pages; International audience; Mount Kenya is an equatorial mountain whose climatic setting is fairly simple (two rainy seasons in March–May, the Long Rains, and October–December, the Short Rains) though concealing significant spatial variations related to elevation and aspect (part I, Camberlin et al., 2014). This part II is dedicated to the sensitivity of sorghum yields to climate variability in space and time, with a focus on the intra-seasonal characteristics of the rainy seasons. To that aim we use the crop model SARRA-H calibrated for the region and fed with rainfall, temperature, wind speed, humidity and solar radiation data over the period 1973–2001 at three stations located on t…
Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.
2009
An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secondary to myelin damage, as generally assumed. SPMS serum affects the perme…
Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes
2019
Development of biocompatible and functional scaffolds for tissue engineering is a major challenge, especially for development of polarised epithelia that are critical structures in tissue homeostasis. Different in vitro models of the lung epithelial barrier have been characterized using non-degradable polyethylene terephthalate membranes which limits their uses for tissue engineering. Although poly-L-lactic acid (PLLA) membranes are biodegradable, those prepared via conventional Diffusion Induced Phase Separation (DIPS) lack open-porous geometry and show limited permeability compromising their use for epithelial barrier studies. Here we used PLLA membranes prepared via a modification of the…
Early signaling network in tobacco cells elicited with methyl jasmonate and cyclodextrins.
2012
We analyze, for the first time, the early signal transduction pathways triggered by methyl jasmonate (MJ) and cyclodextrins (CDs) in tobacco (Nicotiana tabacum) cell cultures, paying particular attention to changes in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)), the production of hydrogen peroxide (H(2)O(2)) and nitric oxide (NO), and late events like the induction of capsidiol. Our data indicate that MJ and CDs trigger a [Ca(2+)](cyt) rise promoted by Ca(2+) influx through Ca(2+)-permeable channels. The joint presence of MJ and CDs provokes a first increase in [Ca(2+)](cyt) similar to that observed in MJ-treated cells, followed by a second peak similar to that found in the presence…
Two Borderline Works: The Miniature Classical Friezes by John Henning
2020
The plaster replicas of two miniaturized reproductions of the Parthenon and Bassae friezes are preserved at the Plaster Cast Gallery of the Department Cultures and Societies of the University of Palermo. The original reproductions were made by the Scottish carver John Henning in the first decades of the 19th century. These artefacts, although they have seldom captured the attention of the archaeologists, are of particular interest and from manifold points of view.
Quality controls for cell cultures: identification of interspecies cross-contamination by PCR-RFLP analysis of the cytochrome b gene
2012
Cross-contaminations of a cell line with cells of different species represent a potential risk in laboratories handling human and animal cells. Therefore, it is necessary to control such contaminations. Tests based on mitochondrial DNA (mtDNA) are used in forensic analysis, phylogenetic studies and in food authentication. However, the use of mtDNA in quality controls of cell cultures is recent. Mitochondrial sequence differences of closely related animal species are five- to tenfold higher than those of nuclear genes. On the contrary, intraspecies variation in mitochondrial sequences is low in most animal species. Moreover, each cell contains 100–10.000 mitochondrial genomes. The amount of …
APOBEC4 Enhances the Replication of HIV-1
2016
APOBEC4 (A4) is a member of the AID/APOBEC family of cytidine deaminases. In this study we found a high mRNA expression of A4 in human testis. In contrast, there were only low levels of A4 mRNA detectable in 293T, HeLa, Jurkat or A3.01 cells. Ectopic expression of A4 in HeLa cells resulted in mostly cytoplasmic localization of the protein. To test whether A4 has antiviral activity similar to that of proteins of the APOBEC3 (A3) subfamily, A4 was co-expressed in 293T cells with wild type HIV-1 and HIV-1 luciferase reporter viruses. We found that A4 did not inhibit the replication of HIV-1 but instead enhanced the production of HIV-1 in a dose-dependent manner and seemed to act on the viral L…
Romas vēsture: 1. grāmata. Ķēniņu laikmets Romā; 21. grāmata. Hannibala iebrukums Itālijā
1940
Saturs : Tits Līvijs. — A.Biogrāfija 7 Tits Līvijs. — B. Romas vēstures pirmā grāmata 13 Romas vēstures divdesmit pirmā grāmata 94 Pielikumi: 1. Horātija Izvēlētas odas (ar biogrāfiju) 67 2. Vokābuļu frekvences saraksts 182 3. Romiešu literatūras konspekts 187
Democracia, república y federación en época isabelina. Una aproximación al proyecto federal de Roque Barcia Martí = Democracy, Republic and Federatio…
2016
Este artículo propone una aproximación al proyecto federal defendido por Roque Barcia Martí a mediados del siglo XIX, prestando especial atención a los principios teóricos y los argumentos –inspirados por una fuerte religiosidad– que sustentan su propuesta. Más allá de la estricta cuestión territorial o de las plasmaciones jurídico-políticas concretas, la federación aparece como una solución global a las tensiones de la modernidad que el Estado liberal no sabía solucionar. En este planteamiento, el campo político se configura como un espacio de humanización que reclama, como punto de partida, la necesaria reformulación política del Estado para transformar la sociedad y, potencialmente, la h…
The Use of Mixed Populations of
2017
Saccharomyces cerevisiae is the most widespread microorganism responsible for wine alcoholic fermentation. Nevertheless, the wine industry is currently facing new challenges, some of them associate with climate change, which have a negative effect on ethanol content and wine quality. Numerous and varied strategies have been carried out to overcome these concerns. From a biotechnological point of view, the use of alternative non-Saccharomyces yeasts, yielding lower ethanol concentrations and sometimes giving rise to new and interesting aroma, is one of the trendiest approaches. However, S. cerevisiae usually outcompetes other Saccharomyces species due to its better adaptation to the fermenta…