Search results for " entry"
showing 10 items of 131 documents
Viral Entry and Receptors
2007
Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear Delivery
2019
Parvoviruses are an important platform for gene and cancer therapy. Their cell entry and the following steps, including nuclear import, are inefficient, limiting their use in therapeutic applications. Two models exist on parvoviral nuclear entry: the classical import of the viral capsid using nuclear transport receptors of the importin (karyopherin) family or the direct attachment of the capsid to the nuclear pore complex leading to the local disintegration of the nuclear envelope. Here, by laser scanning confocal microscopy and in situ proximity ligation analyses combined with coimmunoprecipitation, we show that infection requires importin β-mediated access to the nuclear pore complex and …
Asymmetric synthesis of functionalized tetrahydrofluorenones via an NHC-catalyzed homoenolate Michael addition
2018
The first example of an N-heterocyclic carbene-catalyzed asymmetric desymmetrization of enal-tethered cyclohexadienones via an intramolecular homoenolate Michael addition/esterification reaction is described. This new protocol offers a direct entry to various functionalized tetrahydrofluorenones with three contiguous stereocenters in high yields, good diastereoselectivities and excellent enantioselectivities.
Towards therapeutic gene delivery to human cancer cells : targeting and entry of baculovirus
2008
Geeniterapia on yksi tulevaisuuden hoitomuodoista taistelussa syöpää vastaan. Virukset ovat evoluution myötä kehittyneet tehokkaiksi geeninsiirtäjiksi ja ovat nykyään käytetyimpiä geeninsiirtovektoreita. Yksi geenihoitojen yleistymisen esteistä on kuitenkin ollut virusvektoreiden puutteellinen kohdennus haluttuun kudokseen. Kohdentaminen on erityisen tärkeää syövän hoidossa, jotta hoito vaikuttaisi pääasiassa pahanlaatuisiin soluihin.Mäkelä kehitti tutkimuksessaan syöpäsoluihin kohdentuvia bakulovirusperäisiä geeninsiirtovektoreita. Hän tutki myös viruksen sisäänmenomekanismeja ja kulkeutumista kohdesoluissa.- Hyönteisiä infektoiva bakulovirus on luontaisesti vaaraton ihmisille. Se on helpo…
2014
Human papillomaviruses (HPV) are non-enveloped DNA tumor viruses that infect skin and mucosa. The most oncogenic subtype, HPV16, causes various types of cancer, including cervical, anal, and head and neck cancers. During the multistep process of infection, numerous host proteins are required for the delivery of virus genetic information into the nucleus of target cells. Over the last two decades, many host-cell proteins such as heparan sulfate proteoglycans, integrins, growth factor receptors, actin and the tetraspanin CD151 have been described to be involved in the process of infectious entry of HPV16. Tetraspanins have the ability to organize membrane microdomains and to directly influenc…
Entry Barriers of Small and Medium-sized Software Firms in the Japanese Market
2007
The Japanese software market size was 131,773 million U.S. dollars in 2004. Due to limited domestic software production, Japan is highly dependent on imported software products. Despite the market potential for foreign software firms in Japan, almost no research exists on what kind of challenges foreign software firms encounter when they are entering the market. To fill this gap, this paper investigates the entry barriers of small and medium-sized software firms in the Japanese market by using a multi case-study. The findings suggest that most of the barriers are firm-specific and mainly related to firms’ resources and capabilities to operate in the market. The entry barriers encountered al…
Causation and Effectuation in the Foreign Market Entry and Selection of Software SMEs
2012
Current research emphasizes several factors impacting on SME internationalization. However, it remains unclear that how entrepreneurs in SMEs think and implement their internationalization and select target countries for foreign operations. In this qualitative case study, foreign market selection and entries of five Finnish software firms are analyzed by using the theory of causation and effectuation. The findings imply that software SMEs used more causation logic in their foreign market selection and effectuation logic in their foreign market entries. The case firms that followed causation logic in FMS entered more distant countries. peerReviewed
Incidence of potential drug interactions in a transplant centre setting and relevance of electronic alerts for clinical practice support
2013
Background Adverse drug events may occur as a result of drug–drug interactions (DDIs). Information technology (IT) systems can be an important decision-making tool for healthcare workers to identify DDIs.Objective The aim of the study is to analyse drug prescriptions in our main hospital units, in order to measure the incidence and severity of potential DDIs. The utility of clinical decision-support systems (CDSSs) and computerised physician order entry (CPOE) in term of alerts adherence was also assessed. DDIs were assessed using a Micromedex healthcare series database.Methods The system, adopted by the hospital, generates alerts for prescriptions with negative interactions and thanks to a…
Market Entry and Priority of Small and Medium-Sized Enterprises in the Software Industry: An Empirical Analysis of Cultural Distance, Geographical Di…
2007
This paper investigates the influence of cultural distance, geographical distance, and three market size variables to the target country preference of SMEs in the software industry. In addition, we examine the shift of priorities in SMEs’ country selection by analyzing how these factors impact the selection of the first, second, and third target countries. Empirical findings of this study suggest that almost 70% of country choices can be explained by software market size and geographical distance alone. The findings adduced also that entry priority of SMEs shifts very fast from countries within a short geographical distance to countries with high purchasing power and within a greater geogra…
Entry into Geographically and Psychically Distant Foreign Markets by Small and New Ventures
2008
Paper presented at the 16th Conference on Pacific Basin Finance, Economics, Accounting and Management (PBFEAM), Brisbane, Queensland, Australia, July 2-4.2008. (Key-note paper) peerReviewed