Search results for " envelope"

showing 10 items of 253 documents

Small RNA‐binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli

2019

Abstract The RNA‐binding protein RapZ cooperates with small RNAs (sRNAs) GlmY and GlmZ to regulate the glmS mRNA in Escherichia coli. Enzyme GlmS synthesizes glucosamine‐6‐phosphate (GlcN6P), initiating cell envelope biosynthesis. GlmZ activates glmS expression by base‐pairing. When GlcN6P is ample, GlmZ is bound by RapZ and degraded through ribonuclease recruitment. Upon GlcN6P depletion, the decoy sRNA GlmY accumulates through a previously unknown mechanism and sequesters RapZ, suppressing GlmZ decay. This circuit ensures GlcN6P homeostasis and thereby envelope integrity. In this work, we identify RapZ as GlcN6P receptor. GlcN6P‐free RapZ stimulates phosphorylation of the two‐component sy…

Small RNAsmall regulatory RNAcell envelope precursor glucosamine‐6‐phosphatemedicine.disease_causenegative feedback loopmetabolite sensing0302 clinical medicinetwo-component system QseE-QseFRNA-binding protein RapZRNA‐binding protein RapZGlucosamine0303 health sciencesbiologyEscherichia coli ProteinsGeneral NeuroscienceRNA-Binding ProteinsArticlesRNA BiologyMicrobiology Virology & Host Pathogen InteractionReceptors AdrenergicCell biologyDNA-Binding ProteinsRNA BacterialTransfer RNAPhosphorylationCell envelopeSignal TransductionGlucose-6-PhosphateGeneral Biochemistry Genetics and Molecular BiologyArticletwo‐component system QseE‐QseF03 medical and health sciencesBacterial Proteinscell envelope precursorEscherichia colimedicineRNA MessengerRibonucleaseMolecular BiologyEscherichia coli030304 developmental biologyMessenger RNAGeneral Immunology and MicrobiologyBinding proteinsmall RNAs GlmY and GlmZGene Expression Regulation BacterialMicroreviewRNA binding proteincell envelope precursor glucosamine-6-phosphatetwo-component systembiology.proteinRNA Small Untranslated030217 neurology & neurosurgeryThe EMBO Journal
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Multi-epoch VLTI-PIONIER imaging of the supergiant V766 Cen

2017

Context. The star V766 Cen (=HR 5171A) was originally classified as a yellow hypergiant but lately found to more likely be a 27-36 M red supergiant (RSG). Recent observations indicated a close eclipsing companion in the contact or common-envelope phase. Aims. Here, we aim at imaging observations of V766 Cen to confirm the presence of the close companion. Methods. We used near-infrared H-band aperture synthesis imaging at three epochs in 2014, 2016, and 2017, employing the PIONIER instrument at the Very Large Telescope Interferometer (VLTI). Results. The visibility data indicate a mean Rosseland angular diameter of 4.1 ± 0.8 mas, corresponding to a radius of 1575 ± 400 R. The data show an ex…

Stars: imagingAperture synthesisBinaries: eclipsingFOS: Physical sciencesAstrophysics01 natural sciences010309 opticsCommon envelopeBinaries: closeAngular diameter0103 physical sciencesmassive [Stars]Red supergiantStars: massiveYellow hypergiant010303 astronomy & astrophysicsSolar and Stellar Astrophysics (astro-ph.SR)PhysicsVery Large Telescopeeclipsing [Binaries]Astronomy and AstrophysicsRadiusAstrophysics - Solar and Stellar AstrophysicsSupergiantsSpace and Planetary Scienceimaging [Stars]Techniques: interferometricinterferometric [Techniques]Supergiantclose [Binaries]Astronomy & Astrophysics
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Molecular study of porcine transmissible gastroenteritis virus after serial animal passages revealed point mutations in S protein

2010

Porcine respiratory coronavirus is related genetically to porcine transmissible gastroenteritis virus with a large deletion in S protein. The respiratory virus is a mutated form that may be a consequence of the gastroen- teritis virus's evolution. Intensive passages of the virus in its natural host may enhance the appearance of mutations and therefore may contribute to any attenuated form of the virus. The objective of this study was to characterize the porcine transmissible gastroenteritis virus TMK22 strain after passages in piglets from 1992 until 2007. A typical experimental infection, molecular characterization, and serological analysis were also carried out to further char- acterize a…

SwineSequence analysisvirusesMolecular Sequence DataRT-PCRBiologymedicine.disease_causeArticleVirusViral Envelope ProteinsImmunityVirologyGeneticsmedicineAnimalsPoint MutationDNA sequencingAmino Acid SequenceExperimental infectionPorcine diseaseMolecular BiologyPeptide sequenceCells CulturedCoronavirusMembrane GlycoproteinsGastroenteritis Transmissible of SwineSequence Analysis RNAPoint mutationTransmissible gastroenteritis virusGeneral MedicineVirologyGastroenteritisSpike Glycoprotein CoronavirusRNA ViralRespiratory virusPorcine Respiratory CoronavirusVirus Genes
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Integrazione di celle fotovoltaiche ibride nel vetromattone

2012

L’invenzione riguarda l’integrazione del vetromattone con celle solari ibride (organiche/inorganiche), conosciute come Dye-sensitized Solar Cell (DSC). La combinazione del vetromattone con le DSC consente di migliorare le prestazioni del prodotto originario rendendolo in grado di produrre energia pulita. Le caratteristiche di trasparenza e isolamento termo-acustico del prodotto possono essere regolate agendo sulla configurazione delle DSC. Il prodotto può essere assemblato a formare pannelli fotovoltaici per la realizzazione di facciate traslucide energeticamente “attive” in varie condizioni luminose (condizioni di luce diffusa o artificiale) e indipendentemente dall’angolo di radiazione so…

The integration of Dye-sensitized Solar Cell (DSC) with the glassblock is disclosed. The combination of the two technological elements the glassblock and the DSC module enhance their own peculiarities. The final complete product is ready-made for installation owns the qualities of the glassblock and produces sustainable energy at the same time. The transparency and the thermo-acoustic insulation may be modulated according to the specific requirements of each case of installation. The product may be used for example to create PV panels for glazed and translucent façades that are able to perform efficiently in all light conditions (even in diffuse light also converting internal artificial light) that are not dependant on the angle of the solar radiation and do not suffer from the high operative temperatures of functioning due to the peculiarities of the DSCs. DSCs are made of economic and biocompatible materials and are produced with low-cost and highly productive printing methods on rigid or flexible substrates. With the integration of DSC modules with the glassblock PV panels coincide with the envelope itself so that they do not need any further structures to be installed on and allow the possibility of subtract from the price of the panel the price of the substituted building element allowing further economies. DSCs can be printed in different colours and with various patterns offering to designers and customers the possibility of intervening on the glassblock aesthetical aspect also taking advantage of the peculiar modularity of the glassblock for larger compositions of drawings on the building’s façade. Seven Hypotheses of integration are discussed in order to obtain DSC-integrated glassblock ready for the construction of a precast and dry-assembled PV panel which can use the horizontal and vertical cavities between the glassblocks to house plastic profiles where the electric interconnections and the steel bars -useful for improve the mechanical resistance of the panel- are integrated.Settore ICAR/10 - Architettura Tecnica
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Pannello precompresso di vetromattoni assemblati a secco per la realizzazione di involucri traslucidi

2012

L’invenzione riguarda la realizzazione di un pannello di vetromattoni montati “a secco” -da impiegare per la realizzazione di involucri edilizi traslucidi anche nel caso di edifici alti- che è in grado di rispondere adeguatamente alle sollecitazioni orizzontali (quali quelle dovute a vento e sisma) grazie all’applicazione della tecnica della precompressione mondirezionale. Per offrire una risposta ai limiti imposti dalla tecnica di assemblaggio tradizionale dei vetromattoni (“ad umido”), l’applicazione della precompressione consente, infatti, di dare ai pannelli preassemblati “a secco” una rigidezza adeguata anche in direzione normale al loro piano di posa, sfruttando le buone caratteristic…

The invention is related to the construction of a glassblock panel “dry assembled” that can be used to build translucent building envelope even in high-rise buildings. This panel is able to contrast the horizontal actions due to wind and earthquake by means of the application of unidirectional pre-stressing technique. To overcame the limits due to the “wet assembly” system traditionally used to assemble the glassblocks the use of prestressing technique allow the panels to have an adequate mechanical resistance against the actions perpendicular to their installation plane. In this configuration the mechanical resistance of the glassblocks is exploited. The unidirectional prestressing (along the prevalent dimension of the panel) is obtained through the grip of the nuts inserted in the steel bars that are housed inside the glassblocks supporting profiles positioned in the vertical cavities between the glassblocks and passing through the horizontal profiles useful to separate glassblock rows from each other. The proper nuts grip opposing to the steel plates positioned at the top and bottom of the panel allow to prestress the panel. The steel plate are positioned on a plastic plate fixed to the lateral faces of the glassblocks by means of resins.Settore ICAR/10 - Architettura Tecnica
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Experimental Evidence on the Thermal Performance of Opaque Surfaces in Mediterranean Climate

2013

The thermal insulation of buildings, intended as wrap feature which determines the dispersion of heat, the reference parameter is necessary to contain the thermal losses during the winter season. The transmittance of the opaque components, used as an indicator of the energy quality of a casing, together with the overall coefficient of dispersion, represents a proper descriptor of the behavior during the heating season. However, if a strong insulation in winter conditions brings only positive effects, the same cannot be said for the summer conditions. A high value of the insulation in the casing is convenient only when the gains free, either in the form of solar contribution that of endogeno…

Thermal insulation Energy performance of building Opaque surfaces Thermophysical of involucrOpacityMeteorologybusiness.industryGeneral EngineeringEnergy consumptionDynamic insulationThermal insulationThermalTransmittanceEnvironmental sciencebusinessCasingBuilding envelopeAdvanced Materials Research
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Energy requirement and kinetics of transport of poly(A)-free histone mRNA compared to poly(A)-rich mRNA from isolated L-cell nuclei.

1989

ATP-promoted efflux of poly(A)-rich RNA from isolated nuclei of prelabeled mouse lymphoma L5178y cells has an activation energy of 51.5 kJ/mol, similar to that found for the nuclear envelope nucleoside triphosphatase (48.1 kJ/mol) assumed to be involved in mediating nucleocytoplasmic transport of at least some RNA. Here we show that efflux of two specific poly(A)-rich mRNAs (actin and beta-tubulin) from isolated L-cell nuclei is almost totally dependent on the presence of ATP, while efflux of poly(A)-free histone mRNA (H4, H2B, and H1) also occurs to a marked extent in the absence of this nucleotide. Measurements of temperature dependence of transport rate revealed an activation energy of 5…

Transcription GeneticNuclear EnvelopeRNA transportBiochemistryHistonesMiceAnimalsNucleotideRNA MessengerBinding siteLeukemia L5178Actinchemistry.chemical_classificationCell NucleusMessenger RNALeukemia ExperimentalbiologyRNANucleic Acid HybridizationRibonucleotidesBlotting NorthernMolecular biologyKineticsHistoneEnzymechemistrybiology.proteinEnergy MetabolismPoly APlasmidsEuropean journal of biochemistry
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“Building Integrated Photovoltaics and DSSC-Glassblocks"

2012

According to many market analysts, Building Integrated Photovoltaics (BIPV) represents the future of photovoltaic (PV) industry as well as one of the strategies for the achievement of zero-energy building standards and UE goals for the next years. Dye-sensitised solar cell (DSSC) is a low-cost and very versatile solar technology, offering a wide range of possibilities in terms of colour, transparency and design: hence DSSC are particularly suited for BIPV and could give a big contribution in this direction. Starting from those considerations, this paper will show the first results of a research aimed at the integration of DSSC with the glassblock for the construction of multifunctional tran…

Translucent Building Envelope Glassblocks BIPV DSSCSettore ICAR/10 - Architettura Tecnica
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Longitudinal analysis of Mycobacterium tuberculosis 19-kDa antigen-specific T cells in patients with pulmonary tuberculosis: association with disease…

2003

CD8(+) T cells play a central role in immune protection against infection with Mycobacterium tuberculosis. One of the target epitopes for anti-M. tuberculosis directed CD8(+) T cells is the HLA-A2-restricted 19-kDa lipoprotein peptide VLTDGNPPEV. T cell clones directed against this epitope recognized not only the nominal peptide ligand, but also a closely related peptide (VPTDPNPPEV) from the HIV envelope gp120 (HIV(env) gp120) protein characterized by IFN-gamma release. This cross-reactivity was confirmed in ex vivo in M. tuberculosis 19-kDa tetramer-sorted T cells from patients with tuberculosis and in HIVgp120 tetramer-reactive T cells sorted from HIV(+) patients. M. tuberculosis 19-kDa …

TuberculosisHIV AntigensT cellImmunologyEpitopes T-LymphocyteHIV InfectionsCD146 AntigenBiologyCD8-Positive T-LymphocytesCross ReactionsHIV Envelope Protein gp120medicine.disease_causeEpitopeMycobacterium tuberculosisInterferon-gammaViral ProteinsAntigenBacterial ProteinsAntigens CDT-Lymphocyte SubsetsHLA-A2 AntigenmedicineImmunology and AllergyHumansTuberculosisLongitudinal StudiesNeural Cell Adhesion MoleculesAntigens BacterialMembrane GlycoproteinsMolecular MimicryGranulocyte-Macrophage Colony-Stimulating FactorT lymphocyteMycobacterium tuberculosisOncogene Proteins Viralmedicine.diseasebiology.organism_classificationVirologyPeptide FragmentsDNA-Binding ProteinsMolecular mimicrymedicine.anatomical_structureImmunologyInterleukin-4CD8BiomarkersEuropean journal of immunology
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Association of AUUUA-binding Protein with A + U-rich mRNA during nucleo-cytoplasmic transport

1992

Resealed nuclear envelope (NE) vesicles from rat liver containing entrapped exogenous RNA were used to study the effect of adenosine+uridine binding factor (AUBF), present in cytosolic cell extracts, on ATP-dependent transport of A+U-rich RNA (AU+RNA) and A+U-free RNA (AU-RNA) across the NE. This factor specifically binds to A+U-rich sequences present in the 3' untranslated regions of lymphokine and cytokine mRNAs, containing overlapping AUUUA boxes (granulocyte-macrophage colony stimulating factor, interleukin-3). Addition of AUBF to the extravesicular compartment markedly increased the efflux of the in vitro transcribed, capped and polyadenylated AU+ RNAs. Export of entrapped AU- control …

Untranslated regionCytoplasmAdenosineTranscription GeneticPolyadenylationNuclear EnvelopeMolecular Sequence DataRNA-binding proteinBiologyCell LineStructural BiologyTranscription (biology)EndoribonucleasesAnimalsHumansNuclear MatrixRNA MessengerBinding siteNuclear export signalUridineMolecular BiologyCell NucleusMessenger RNABinding SitesBase SequenceGranulocyte-Macrophage Colony-Stimulating FactorInterferon-alphaRNA-Binding ProteinsRNAMolecular biologyRatsKineticsLiverRibonucleoproteinsInterleukin-3Carrier ProteinsPlasmidsPolyribonucleotidesProtein BindingJournal of Molecular Biology
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