Search results for " enzyme"

Changes in physico-chemical traits and enzymes oxidative system during cold storage of 'Formosa' papaya fresh cut fruits grown in the mediterranean a…

2020

In this study, the effects of cold storage (5 +/- 0.5 degrees C and relative humidity of 90 +/- 1%) on the quality of fresh papaya slices packed in a passive atmosphere with a semi-permeable film were evaluated. Physico-chemical traits such as total soluble solids, reducing sugar, pH increased during storage as well as the polyphenols, carotenoid content and antioxidant activity that reaching the highest values at end of trials. Changes in colorimetric parameters resulted in a significant decrease after 4 days of hue angle values, which then remained constant. The cutting process enhanced the antioxidant enzymes activity such as superoxide dismutase, catalase and ascorbate peroxidase. The a…

Integrated Strategies to Improve the Quality of Wheat End-Products: Optimization of Processes and Development of Functional Food

Strategie integrate per il miglioramento qualitativo di prodotti da forno a base di grano: ottimizzazione dei processi produttivi e sviluppo di alimenti funzionali. Sono state sviluppate tecnologie applicate ai prodotti da forno per il miglioramento dei processi produttivi e lo studio di alimenti funzionali. In particolare è stato condotto uno studio su trattamenti sanitizzanti al fine di ridurre le contaminazioni microbiche (soprattutto lieviti) sul prodotto finito. E’ stata proposta una soluzione a base di perossido e argento colloidale in confronto con soluzioni fenoliche OPP. Il monitoraggio, biennale, è stato svolto nelle celle di lievitazione e raffreddamento del prodotto finito (pane…

A study of the pattern of digestive enzimes in Diplodus puntazzo (Cetti, 1777) (Osteychthyes, Sparidae): evidence for the definition of nutritional p…

2005

The digestive enzymes (proteases, carbohydrases and lipases) present along the alimentary tract of juveniles and adults of Diplodus puntazzo were studied. The data obtained showed a different distribution of the enzymatic activities in the different parts of the alimentary tract. Levels of enzymatic activity in the adults were higher than those measured in juveniles, suggesting that adults have a greater ability to digest larger pieces of food. The enzymatic pattern of D. puntazzo justifies its omnivorous habit and suggests an high potential for digesting vegetable polysaccharides. The results of this study suggest the need to adapt the diet to the digestive potential of this new farming sp…

Mixed Function Oxidase Activity and Organochlorine Levels in Farmed Sharpsnout Seabream (Diplodus puntazzo) from Two Intensive Aquaculture Facilities

2004

Mixed function oxidase (MFO) activities and organochlorine levels were investigated in liver of farmed sharpsnout seabream bred in two intensive facilities, a sea farm and a land-based farm. The aim of this study is to investigate how different facility locations and breeding conditions might affect fish metabolic capabilities and accumulation of toxic chemicals in farmed sharpsnout seabream. The differences between breeding two or more fish species in the same cage or tank known as polyculture, and monoculture (1 species) were also investigated. The results showed that both facility location (sea and land) as well as breeding systems (polyculture and monoculture) might be responsible for t…

Digestive Enzymes in Larvae and Juveniles of Farmed Sharpsnout Seabream (Diplodus puntazzo) (Cetti, 1777)

2011

The ontogenetic development and pattern of main digestive enzyme activities (proteases, carbohydrases and lipases) were investigated in farmed sharpsnout seabream Diplodus puntazzo larvae and juveniles, during the passage from the larval stage to adult (from 21 to 277 days after hatching - DAH). Larvae showed exponential growth, thus confirming that Rotifers and Artemia nauplii are suitable preys for early feeding of D. puntazzo larvae. Pepsin activity was low in the larvae, but it increased afterwards in relation with stomach development. In juveniles, levels of specific activity of trypsin, chymotrypsin and elastase were lower compared to that recorded in larvae. A decrease in the activit…

A new method to value efficiency of enzyme blends for pancreas tissue digestion

2010

In pancreatic islets isolation for cell therapy the major enzymes used are obtained from Clostridium hystoliticum: class I and class II collagenases. They are used in a defined tissue dissociation enzyme mixture together with neutral protease (Dispase) or thermolysin (Thermostable Neutral Protease). However, just to now, people working in islets production found variable outcomes in isolation procedures mainly due to large variability in enzymatic blend composition and efficacy. Using electrophoresis and gelatin zymography approaches together with densitometry evaluation assays we compared the composition, stability and auto-digestion processes of C. hystoliticum collagenases, Neutral prote…

Ring opening polymerization of cyclic esters with metal free catalysts

2012

The role of polymorphisms of thiopurine methyltransferase in therapy with Azathioprine: preliminary study Journal of Biological Research

2018

Azathioprine is an immunosuppressant drug belonging to the class of thiopurines widely used in clinical therapy. Its immunosuppressive action is linked to the substantial action mechanism in the inhibition of the synthesis of nitrogenous bases purine carried out in T-lymphocyte. The level of such medication limit resides in side effects such as myelosuppression and the development of tumours. The occurrence of side effects is linked to the presence of genetic polymorphisms of Thiopurine methyltransferase (TPMT). To date, 40 allelic variants for TPMT have been detected. However, those responsible for the reduction of enzyme activity are three: *2, *3A, *3C. The presence of one of the three p…

Genotyping of GII.4 and GIIb norovirus RT-PCR amplicons by RFLP analysis

2007

GII.4 and GIIb/Hilversum norovirus (NoV) strains appear to have a prominent epidemiological role in outbreaks or sporadic cases of human gastroenteritis. Sequence analysis, although laborious, is the reference method used for characterization of noroviruses. In this study a screening test is proposed to characterize GIIb and GII.4 NoVs based on restriction fragment length polymorphism (RFLP) analysis of amplicons obtained from the RNA-dependent RNA polymerase (RdRp) region. Virtual analysis of 793 RdRp sequences of GGI and GGII NoVs, retrieved from GenBank, and representative of global geographical origins on a long-time period, permitted the selection of four restriction enzymes, XmnI, Ahd…

Therapeutic management of elderly hypertensives with concomitant ischaemic heart disease.

1992

CHARACTERISTICS OF ELDERLY HYPERTENSIVES: Hypertension is most prevalent in older patients and is associated with increasing morbidity and mortality with age. Elderly hypertensives often suffer from concomitant diseases, such as ischaemic heart disease, caused by age-induced modifications to the cardiovascular system, haemodynamic function and neurohormone activity. THERAPEUTIC MANAGEMENT: Therapeutic management of elderly hypertensives with concomitant ischaemic heart disease must take account of the pharmacodynamic and pharmacokinetic changes that occur in older subjects and drugs should be selected for their efficacy in both diseases. Since elderly subjects often metabolize drugs more sl…