Search results for " frames"

Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy

2020

AbstractThe current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and t…

Nucleotide sequence of a ssRNA phage from Acinetobacter: kinship to coliphages.

2002

The complete nucleotide sequence of ssRNA phage AP205 propagating in Acinetobacter species is reported. The RNA has three large ORFs, which code for the following homologues of the RNA coliphage proteins: the maturation, coat and replicase proteins. Their gene order is the same as that in coliphages. RNA coliphages or Leviviridae fall into two genera: the alloleviviruses, like Qβ, which have a coat read-through protein, and the leviviruses, like MS2, which do not have this coat protein extension. AP205 has no read-through protein and may therefore be classified as a levivirus. A major digression from the known leviviruses is the apparent absence of a lysis gene in AP205 at the usual positio…

A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation.

2002

Histone mRNAs accumulate in the S phase and are rapidly degraded as cells progress into the G(2) phase of the cell cycle. In Saccharomyces cerevisiae, fusion of the 3' untranslated region and downstream sequences of the yeast histone gene HTB1 to a neomycin phosphotransferase open reading frame is sufficient to confer cell cycle regulation on the resulting chimera gene (neo-HTB1). We have identified a sequence element, designated the distal downstream element (DDE), that influences both the 3'-end cleavage site selection and the cell cycle regulation of the neo-HTB1 mRNA. Mutations in the DDE, which is located approximately 110 nucleotides downstream of the HTB1 gene, lead to a delay in the…

Identification ofCandida albicansclinical isolates by PCR amplification of anEFB1gene fragment containing an intron-interrupted open reading frame

2000

The use of a single pair of primers, deduced from the intron and exon nucleotide sequences of the Candida albicans EFB1 gene, in polymerase chain reaction (PCR) assays performed with whole cells of both laboratory strains and clinical isolates of Candida species, resulted in the species-specific amplification of a 785 bp DNA fragment in C. albicans strains. Clinical C. albicans isolates were tested, and 85 out of 86 generated the expected PCR-amplified product; other Candida species, both laboratory strains and clinical isolates, as well as laboratory strains belonging to other fungal genera, including medically relevant taxa, failed to amplify any DNA fragment. In addition, unusual C. albi…

Granice romantyzmu w dziejach polskiej komedii według dziewiętnastowiecznej krytyki krajowej

2021

W polskiej krajowej krytyce literackiej i teatralnej XIX w., dokonującej już pewnych bilansów dorobku romantyzmu, pojęcia „komedii romantycznej” i „romantyzmu w komedii” służyły za środek do porównawczych analiz i historycznoliterackich periodyzacji, choć jednocześnie takie kwestie, jak semantyczna zawartość tych terminów czy granice czasowe określanego nimi zjawiska, nie były precyzowane ani nawet problematyzowane. Podjęta w niniejszym artykule próba rekonstrukcji ówczesnych niewypowiedzianych wyobrażeń o tych znaczeniach i granicach wiedzie w kierunku formuły chronologicznie rozpiętej między „kosmopolityzmem”, przypisywanym wtedy klasycystycznej komedii XVIII w. i zwalczonym rzekomo dopie…

Chloroplastic glutamine synthetase from Brassica napus.

1993

Reproducing pairs of measurable functions

2017

We analyze the notion of reproducing pair of weakly measurable functions, which generalizes that of continuous frame. We show, in particular, that each reproducing pair generates two Hilbert spaces, conjugate dual to each other. Several examples, both discrete and continuous, are presented.

Extracting (good) discourse examples from an oral specialised corpus of wine tasting interactions

2015

International audience; This article outlines the semi-automated extraction of dictionary examples used in the compilation of a professional online dictionary of wine tasting. Named OenoLex Bourgogne, this dictionary was started to respond to the demand for a lexicographic information tool from the French wine industry of Burgundy, the Bureau Interprofessionnel des Vins de Bourgogne.

Non linear analysis of 3D elastoplastic framed structures

2014

Toward a cognitive Discourses Semantics. Theoreticals inputs and empirical corpus-based case study in German.

2021

At the intersection between usage-based construction grammar and frame semantics, a cognitive semantic analysis is to be theorized. The present work proposes to develop for this purpose the Cognitive Discourse Analysis model. Discourse is then understood following Michel Foucault as a conceptual system based on linguistic, social and epistemic knowledge. Its linguistic operationalization is achieved through the contribution of textual linguistics and corpus following Dietrich Busse. The aim of such a methodological construction is to finely examine a corpus of recurrent texts in a cognitive perspective, and thus as close as possible to the linguistic reality, and to be able represent the en…