Search results for " frames"

Identification of transcribed protein coding sequence remnants within lincRNAs

2018

Abstract Long intergenic non-coding RNAs (lincRNAs) are non-coding transcripts >200 nucleotides long that do not overlap protein-coding sequences. Importantly, such elements are known to be tissue-specifically expressed and to play a widespread role in gene regulation across thousands of genomic loci. However, very little is known of the mechanisms for the evolutionary biogenesis of these RNA elements, especially given their poor conservation across species. It has been proposed that lincRNAs might arise from pseudogenes. To test this systematically, we developed a novel method that searches for remnants of protein-coding sequences within lincRNA transcripts; the hypothesis is that we can t…

Genomic structure and functional characterization of the human ADAM10 promoter

2005

The ADAM10 gene encodes a membrane-bound disintegrin-metalloproteinase, which, after overexpression in an Alzheimer disease (AD) mouse model, prevents amyloid pathology and improves long-term potentiation and memory. Because enhancing ADAM10 expression appears to be a reasonable approach for treatment of AD, we functionally analyzed the ADAM10 gene. Both human and mouse ADAM10 genes comprise approximately 160 kbp, are composed of 16 exons, and are evolutionarily highly conserved within 500 bp upstream of either translation initiation site. By using luciferase reporter assays, we demonstrate that nucleotides -2179 to -1 upstream of the human ADAM10 translation initiation site represent a fun…

An improvement of ComiR algorithm for microRNA target prediction by exploiting coding region sequences of mRNAs

2020

AbstractMicroRNA are small non-coding RNAs that post-transcriptionally regulate the expression levels of messenger RNAs. MicroRNA regulation activity depends on the recognition of binding sites located on mRNA molecules. ComiR is a web tool realized to predict the targets of a set of microRNAs, starting from their expression profile. ComiR was trained with the information regarding binding sites in the 3’utr region, by using a reliable dataset containing the targets of endogenously expressed microRNA in D. melanogaster S2 cells. This dataset was obtained by comparing the results from two different experimental approaches, i.e., inhibition, and immunoprecipitation of the AGO1 protein--a comp…

Genetic organization of the citCDEF locus and identification of mae and clyR genes from Leuconostoc mesenteroides.

1999

ABSTRACT In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme ( mae ) and a putative regulatory protein ( clyR ) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the citrate lyase locus revealed one polycistronic mRNA covering the mae and citCDEF genes. This transcript was detected only on RNA prepared from cells grown in the presence of citrate. Primer extension experiments suggest that clyR and the citrate lyase operon are expressed from a bidirectional A-T-rich promoter region located between mae and clyR.

Dynamic stability of plane elastic frames

1982

Abstract The stability of plane elastic frames subjected to a vertical foundation motion of the stationary, ergodic type is investigated. The equations of motion are obtained in modal co-ordinates, with account taken of many modes of vibration. The problem is subsequently reduced to the study of only the first mode of vibration. By considering a particular case, the stability domains are sketched as functions of the variation of the rigidities of the beam-column connecting joints and as functions of the number of stories.

Prevalence of antibodies to human papillomaviruses in the general population of the Czech Republic.

1998

Sera from 450 individuals between the age of 1 and 80 years, representing the general population of the Czech Republic, were tested for the presence of antibodies to human-papillomavirus(HPV)-derived antigens. The following antigens were used: (i) HPV1 virions; (ii) HPV16, -18 and -33-virus-like particles (VLP); (iii) peptides derived from L2 open reading frames (ORFs) of HPV16 and HPV6/11; (iv) peptides derived from HPV16 E2, E4 and E7 ORFs of HPV16. The prevalence of antibodies reactive with the capsid-derived antigens was age-dependent, while no clear age dependence was observed in the distribution of antibodies to peptides derived from HPV16 early proteins. In individual sera, high corr…

Gabor frames for classification of paroxysmal and persistent atrial fibrillation episodes

2016

[EN] In this study, we propose a new classification method for early differentiation of paroxysmal and persistent atrial fibrillation episodes, i.e. those which spontaneously or with external intervention will return to sinus rhythm within 7 days of onset from the ones where the arrhythmia is sustained for more than 7 days. Today, clinicians provide patients classification once the course of the arrhythmia has been disclosed. This classification problem is dealt with in this study. We study a sparse representation of surface electrocardiogram signals by means of Gabor frames and afterwards we apply a linear discriminant analysis. Thus, we provide an early discrimination, obtaining promising…

Association study of a SNP coding for a M129V substitution in the prion protein in schizophrenia.

2003

Gabor systems and almost periodic functions

2017

Abstract Inspired by results of Kim and Ron, given a Gabor frame in L 2 ( R ) , we determine a non-countable generalized frame for the non-separable space AP 2 ( R ) of the Besicovic almost periodic functions. Gabor type frames for suitable separable subspaces of AP 2 ( R ) are constructed. We show furthermore that Bessel-type estimates hold for the AP norm with respect to a countable Gabor system using suitable almost periodic norms of sequences.

Shear models of Rc-encased steel joist beams in MRFs

2019

This study presents the application of different analytical and finite element (FE) models aimed at predicting the shear resistance of reinforced concrete (RC) and reinforced concrete-encased steel joist (HRCESJ) beams with inclined transversal reinforcement in moment resisting frames (MRFs). In particular, four analytical models are taken into account, two of them specifically conceived for HRCESJ beams in seismic area. The analytical models considered are Eurocode-2 model for the shear strength of RC beams; a variable-inclination stress-field approach; a strut-and-tie additive model and, finally, an analytical formulation in which the shear capacity depends on the number of pairs of incli…