Search results for " fusion"
showing 3 items of 1303 documents
Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes.
2002
<i>Objectives:</i> In an attempt to develop virus-like particles (VLPs) as experimental vaccine against human papilloma virus (HPV)-induced tumours, the HPV16 E7 oncoprotein epitopes spanning amino acid (aa) residues 35–98 were expressed on three proteins capable of VLP formation: hepatitis B virus (HBV) surface (HBs) and core (HBc) antigens, and RNA phage fr coats (frCP). <i>Methods:</i> The profile of immunoglobulin isotypes induced in Balb/C mice after immunization with purified chimeric proteins was studied. <i>Results:</i> The HBs*-E7(35–54) protein expressing E7 residues 35–54 between residues 139 and 142 of the HBs carrier formed HBs-like particles…
Extraction d'un graphe de navigabilité à partir d'un nuage de points 3D enrichis
2017
International audience; Ce travail se place dans le cadre général du projet ANR pLaTINUM lié à la navigation autonome et plus parti-culièrement à la génération de cartes pour la navigation basée perception. Il consiste à développer une nouvelle méthode pour résumer une carte 3D (un nuage dense de points 3D) et extraire un graphe de navigabilité facilitant l'utilisation de cette carte par des systèmes de navigation à ressources matérielles limitées (smart-phones, voitures, robots.. .). Cette méthode vise à ex-traire les régions les plus saillantes de l'environnement étudié afin de construire une carte récapitulative. Ce processus de résumé de carte basé sur la vision est appliqué d'une façon…
α-Conotoxins EpI and AuIB switch subtype selectivity and activity in native versus recombinant nicotinic acetylcholine receptors
2003
The Xenopus laevis oocyte expression system was used to determine the activities of alpha-conotoxins EpI and the ribbon isomer of AuIB, on defined nicotinic acetylcholine receptors (nAChRs). In contrast to previous findings on intracardiac ganglion neurones, alpha-EpI showed no significant activity on oocyte-expressed alpha3beta4 and alpha3beta2 nAChRs but blocked the alpha7 nAChR with an IC50 value of 30 nM. A similar IC50 value (103 nM) was obtained on the alpha7/5HT3 chimeric receptor stably expressed in mammalian cells. Ribbon AuIB maintained its selectivity on oocyte-expressed alpha3beta4 receptors but unlike in native cells, where it was 10-fold more potent than native alpha-AuIB, had…