Search results for " immobilization"
showing 10 items of 30 documents
Enhanced Heterogeneously Catalyzed Suzuki-Miyaura Reaction over SiliaCat Pd(0)
2013
Abstract The SiliaCat Pd(0) solid catalyst can be efficiently employed in the Suzuki–Miyaura cross-coupling of an ample variety of haloarenes, including economically viable chloroarenes. The catalyst can be extensively recycled without loss of activity and with low leaching of valued palladium, opening the route to widespread utilization of the method to afford high yields of biaryls devoid of contaminating by-products.
Revalorization of cellulosic wastes from Posidonia oceanica and Arundo donax as catalytic materials based on affinity immobilization of an engineered…
2020
Catalytic materials obtained by enzyme immobilization have multiple potential applications in the food industry. The choice of the immobilization method and support may be critical to define the properties of the immobilized enzyme compared to the soluble form. Although the use of immobilized enzymes shows multiple advantages, their catalytic efficiency is compromised in many instances. Molecular engineering techniques have been used to generate hybrid proteins where the enzyme of interest is fused to a module with affinity to a specific biopolymer. Binding of the hybrid TmLac-CBM2 protein, in which the β-galactosidase from Thermotoga maritima is fused to a carbohydrate-binding module from …
A Protein-Interaction Array Inside a Living Cell
2013
Cell phenotype is determined by protein network states that are maintained by the dynamics of multiple protein interactions.1 Fluorescence microscopy approaches that measure protein interactions in individual cells, such as by Forster resonant energy transfer (FRET), are limited by the spectral separation of fluorophores and thus are most suitable to analyze a single protein interaction in a given cell. However, analysis of correlations between multiple protein interactions is required to uncover the interdependence of protein reactions in dynamic signal networks. Available protein-array technologies enable the parallel analysis of interacting proteins from cell extracts, however, they can …
The effects of a number of short-term exogenous stimuli on pineal serotonin-N-acetyltransferase activity in rats
1984
The present study deals with the question as to what extent the sympathetically innervated rat pineal gland is affected by a number of short-term exogenous stimuli given during day-time, as assessed by measuring pineal serotonin-N-acetyltransferase activity (NAT) which is directly proportional to melatonin formation. In male Sprague-Dawley rats kept under LD 12:12 pineal NAT was statistically significantly depressed by physical immobilization for 2 hours, swimming for 15 min in water of 10 and 30 degrees C, exposure for 2 hours to cold (5 degrees C) or heat (40 degrees C), noise (90 db) for 2 hours and hunger for 17 hours. An increase in NAT was noted after swimming for 15 min in water of 2…
Effect of PCL/PEG-Based Membranes on Actinorhodin Production in Streptomyces coelicolor Cultivations
2015
The actinomycetes, Gram-positive filamentous bacteria, are the most prolific source of natural occurring antibiotics. At an industrial level, antibiotics from actinomycete strains are produced by means of submerged fermentations, where one of the major factors negatively affecting bioproductivity is the pellet-shaped biomass growth. The immobilization of microorganisms on properly chosen supports prevents cell-cell aggregation resulting in improving the biosynthetic capability. Thus, novel porous biopolymer-based devices are developed by combining melt mixing and particulate leaching. In particular, polycaprolactone (PCL), polyethylene glycol (PEG), and sodium chloride (NaCl) with different…
Splitting of Surface-Immobilized Multicompartment Micelles into Clusters upon Charge Inversion
2016
International audience; We investigate a morphological transition of surface-immobilized triblock terpolymer micelles: the splitting into well-defined clusters of satellite micelles upon pH changes. The multicompartment micelles are formed in aqueous solution of ABC triblock terpolymers consisting of a hydrophobic polybutadiene block, a weak polyanionic poly(methacrylic acid) block, and a weak polycationic poly(2-(dimethylamino)ethyl methacrylate) block. They are subsequently immobilized on silicon wafer surfaces by dip-coating. The splitting process is triggered by a pH change to strongly basic pH, which goes along with a charge reversal of the micelles. We find that the aggregation number…
Magnetic hybrid TiO 2 /Alg/FeNPs triads for the efficient removal of methylene blue from water
2018
A new adsorbent material with combined adsorption, photocatalytic, and magnetic properties has been suc- cessfully synthesized and tested for the efficient dye removal from methylene blue (MB) contaminated water. A facile non-thermal method was applied to synthesize a hybrid nanocomposite consisting of TiO2/calcium algi- nate (TiO/Alg) and magnetite (Fe3O4) nanoparticles (FeNPs). The potential of the adsorbent Alg as a barrier to prevent direct contact between the magnetic core and TiO2 was experimented by varying the synthesis condi- tions. The performance of four differently synthesized TiO2/Alg/FeNPs samples (TiO2/Alg/FeNPs-1, TiO2/Alg/ FeNPs-2, TiO2/Alg/FeNPs-3, and TiO2/Alg/FeNPs-4) wa…
Optical biosensors based on ZnO nanostructures: advantages and perspectives. A review
2016
This review article highlights the application of beneficial physico-chemical properties of ZnO nanostructures for the detection of wide range of biological compounds. As the medical diagnostics require accurate, fast and inexpensive biosensors, the advantages inherent optical methods of detection are considered. The crucial points of the immobilization process, responsible for biosensor performance (biomolecule adsorption, surface properties, surface defects role, surface functionalization etc.) along with the interaction mechanism between biomolecules and ZnO are disclosed. The latest achievements in surface plasmon resonance (SPR), surface enhanced Raman spectroscopy (SERS) and photolumi…
Effect of Streptomyces coelicolor M145 cell immobilization on actinorhodin production
2016
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Fracturas de radio distal. ¿Progresa el desplazamiento entre la segunda y la sexta semana?.
2020
espanolLas fracturas de radio distal son las mas frecuentes en nuestra especialidad. El tratamiento conservador con inmovilizacion, basado en una serie de parametros radiologicos, sigue siendo el mas utilizado hoy en dia. El objetivo de nuestro estudio ha sido evaluar el desplazamiento secundario de estas fracturas entre la segunda y sexta semana de evolucion. Para ello hemos evaluado las fracturas de radio distal tratadas de forma conservadora en nuestro Servicio, durante el ano 2016. De un total de 53 fracturas de muneca tratadas de forma conservadora, un 49% de los casos presento algun tipo de desplazamiento, si bien la mayor parte de estos tuvieron lugar antes de la segunda semana. Nues…