Search results for " kit"

Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens

2021

The performance of 2 antigenic commercial assays for enteric adenovirus (AdV) infection, bioNexia Rota-Adeno ImmunoChromatographic Tests (ICT) and LIAISON® Adenovirus ChemiLuminescence Immuno Assays (CLIA), was evaluated on 321 stools from children hospitalized for acute gastroenteritis in Palermo, Italy, using a Real time-PCR (Rt-PCR) as reference method. The CLIA showed higher sensitivity (77% vs 60%), accuracy (94.4 vs 90.9) and concordance (k: 0.81 vs 0.67) with respect to ICT, despite equivalent specificity (98.8%). Using the Ct values of the Rt-PCR as a proxy of the fecal viral load, similar Ct values (mean 9.32 vs 9.89) were observed among the true positive samples, whilst a signific…

Biogas yield from Sicilian kitchen waste and cheese whey

2013

The aim of this study is to determine the chemical composition of kitchen waste and cheese whey, as well as the biogas yield obtained from the Anaerobic Digestion (AD) tests of these two raw materials. Since the separated waste collection is performed in the town of Marineo (Palermo), a sample of kitchen waste, different from food industry one and included in the Organic Fraction of Municipal Solid Waste (OFMSW), was collected from the mass stored at the households of this town. Moreover, a sample of cheese whey was collected in a Sicilian mini dairy plant, where sheep milk is processed. This investigation was carried out inside laboratory digesters of Aleksandras Stulginskis University (Li…

Valutazione di alcune performance diagnostiche di kit ELISA per la diagnosi sierologica di Anemia Infettiva Equina (AIE)

2012

Data on evaluation of some diagnostic parameters of all ELISA kits available in Italy for the serodiagnosis of AIE are presented and discussed. Ten laboratories were involved using a panel of 30 sera ran with 4 commercial kits and 2 in-house kits. Kits were also evaluated using a panel of sera from vaccinated animals at different days post vaccination (p.v.). All sera were also tested in agar gel immunodiffusion (AGID). The parameters evaluated were: diagnostic sensitivity (DSe) and specificity (DSp), Cohen K, weighted Cohen K, coefficient of variation (CV), accordance, concordance. Analysis of these parameters indicates that all kits have a higher sensitivity than AGID, even if a complete …

Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range

2017

Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Here, we performed an independent comparison between single-pot solid-phase-enhanced sample preparation (SP3), filter-aided sample preparation (FASP), and a commercial kit based on the in-StageTip (iST) method. We assessed their performance for the processing of proteomic samples in the low μg range using varying amounts of HeLa cell lysate (1-20 μg of total protein). All three workflows showed similar performances for 20 μg of starting material. When handling sample sizes below 10 μg, the number of identified proteins and peptides as well as the quantitat…

Home-based HIV testing: Using different strategies among transgender women in Argentina.

2019

Background In Argentina, HIV prevalence among transgender women (TGW) has been reported at 34%. The stigma is one of the most important factors limiting their access to healthcare services. The aims of this study were to compare different HIV testing methodologies, to determine the factors associated with HIV diagnosis and to determine the feasibility of a home-based HIV testing service for TGW. Methods A multidisciplinary team performed home-based HIV testing interventions in four cities of Argentina. Participants self-identified as TGW, older than 14 years and with a negative or unknown HIV status. Blood samples were screened by two rapid tests (RT), one based on antibodies (Determine™ HI…

Status of Red kite in Italy

2009

Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review

2021

Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of AAT in peripheral blood were searched in PubMed in July 2021. The selection criteria included (1) any type of study design that included a quantification of AAT in peripheral blood; (2) studies written in English or Spanish; (3) studies evaluating human beings; and (4) studies involving adults. Results: Out of 207 studies, the most frequently used techniques were nephelometry (43.9%), followed by…

Cookbooks. Narrative spaces and user manuals

2019

Nel sistema che definisce le diverse tipologie di volumi di cui si occupa la grafica editoriale nell’ambito del design della comunicazione visiva, una sezione a sé, con una sua storia, una sua struttura, un suo preciso linguaggio, è quello occupato dai libri di cucina, veri e propri manuali d’uso per la preparazione dei cibi, e così dovrebbero essere sempre chiamati, non semplicemente ricettari, molto più genericamente e riduttivamente, i quali rappresentano degli artefatti atti a contenere non solo pratiche e tecniche ma anche indicazioni relative ad alimenti, dosaggi, tempi, temperature, lavorazioni, procedure, finiture, tutti aspetti questi che li fanno più assomigliare a manuali tecnici…

" KIT TETTOIA COPERTA"

2011

Oceano Mediterraneo : cardboard and plaster architectures

2016

La riflessione si muove dal confronto fra due architetture diverse, eppure simili: la Casa Salem di Pasquale Culotta e Giuseppe Leone e la Kitchen House di Marcello Panzarella. La prima realizzata, tra il 1972 e il 1973, a Cefalù, in contrada Ogliastrillo; la seconda immaginata da Panzarella nel 2011, sul 38° parallelo, forse lungo la costa settentrionale della Sicilia, adagiata su un pendio rivolto verso il mare. Le due descrizioni sono i poli attraverso cui si dipana un ragionamento che trova una parziale e contraddittoria spiegazione nel titolo. Perché, pur nelle differenze, le architetture prese in considerazione costituiscono due modi per tenere insieme una appartenenza mediterranea …