Search results for " life science"

Viral and cellular determinants of hepatitis C virus RNA replication in cell culture.

2003

Studies on the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high level. Analysis of the replicon population in selected cells revealed the occurrence of cell culture-adaptive mutations that enhance RNA replication substantially. To gain a better understanding of HCV cell culture adaptation, we characterized conserved mutations identified by sequence analysis of 26 independent replicon cell clones for their effect on RNA replication. Mutations enhancing replication were found in nearly every nonstructural (NS) protein, and they could be subdivided into at …

Rapid and sensitive detection of metapneumovirus in clinical specimens by indirect fluorescence assay using a monoclonal antibody.

2008

Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of hMPV and to determine its epidemiology. Respiratory samples from 1,386 patients collected during 2 consecutive years were screened for hMPV using indirect immunofluorescence (IFA) assay with a monoclonal antibody. Forty-three patients tested positive for hMPV by the IFA method. In parallel, the samples were examined with RT-PCR on the F gene. Of these, 41 specimens were RT-PCR p…

Immune responses during COVID-19 infection

2020

International audience; Over the past 16 years, three coronaviruses (CoVs), severe acute respiratory syndrome CoV (SARS-CoV) in 2002, Middle East respiratory syndrome CoV (MERS-CoV) in 2012 and 2015, and SARS-CoV-2 in 2020, have been causing severe and fatal human epidemics. The unpredictability of coronavirus disease-19 (COVID-19) poses a major burden on health care and economic systems across the world. This is caused by the paucity of in-depth knowledge of the risk factors for severe COVID-19, insufficient diagnostic tools for the detection of SARS-CoV-2, as well as the absence of specific and effective drug treatments. While protective humoral and cellular immune responses are usually m…

Glie et neurones : vers un partenariat actif influençant le comportement sensoriel chez la drosophile

2014

Anatomic and functional follow-up of patients with an idiopathic epiretinal membrane and a preserved visual acuity

2013

Purpose: To determine the anatomic and functional retinal modifications in patients with an idiopathic epiretinal membrane (ERM) and a preserved visual acuity (VA).Methods: Monocentric, prospective study in 24 eyes of 24 patients with an ERM and a VA better than 20/25. Recorded data were best corrected VA on ETDRS scale, retinal anatomic lesions on spectral-domain OCT, retinal sensitivities on microperimetry and macular and foveal thicknesses at 0 and 6 months.Results: At inclusion, mean VA was 84 ± 5 letters, mean macular sensitivity was 14.0 ± 1.4 decibels (dB) and mean foveal sensitivity was 14,4 ± 1,5 dB. Mean macular thickness was 351 ± 34 µm and mean foveal thickness was 348 ± 66 µm. …

Motion perception in early glaucoma

2013

Purpose: The aim of our study was to underline the changes in the movement perception for early glaucoma. Our working hypothesis consisted in inquiring if the impairment of the magnocellular pathway may modify the movement perception capabilities in the visual field, more particularly in its peripheral area.Methods: We included 14 healthy subjects and 14 patients with early primary open angle glaucoma. A moving target was presented on a semicircular screen (1.8 m diameter); participants were asked to localize the Ending Point (EP) of each movement. Each stimulus consisted in a white dot (0,72° of diameter) moving horizontally with the imposed velocity profile. Two different laws of motion w…

Anticipatory Postural Adjustments and kinematic arm features when postural stability is manipulated

2018

International audience; Beyond the classical paradigm that presents the Anticipatory Postural Adjustments (APAs) as a manner to create forces that counteract disturbances arising from the moving segment during a pointing task, there is a controversial discussion about the role APAs to facilitate the movement and perform a task accurately. In addition, arm kinematics features are classically used to infer the content of motor planning for the execution and the control of arm movements, The present study aimed to disentangle the conflicting role of APAs during an arm-pointing task in which the subjects reach a central diode that suddenly turns on, while their postural stability was manipulate…

Effect of soil management and weed controlm on the diversity and establishment of arbuscular mycorrhizal symbiosis colonisation in Mediterranean clim…

2006

International audience

Pleasure or health: Do children have to choose?

2017

International audience; Pleasure from eating is known to be a strong predictor of children’s food choices. Yet, children’s food preferences generally do not align with dietary recommendations, children rate energy-dense foods as the most liked and vegetables the least liked. In order to incite children to adopt a healthy diet, parents, caregivers, and national campaigns put forward nutritional arguments. However, there is limited research regarding the impact of the level of nutritional concern on children’s food choices, especially compared to pleasure-oriented goals. This talk is an overview of the research work I have conducted during my PhD, and explores the relationships between childr…

A web-based and mobile intervention with brochure support providing complementary feeding guidelines to first-time parents in France: Randomized Cont…

2023

Purpose: In light of the latest epidemiological and nutritional knowledge and scientific evidence supporting responsive feeding, the French feeding guidelines for 0-3 years were recently updated and nationally disseminated through a brochure and media campaign by Santé publique France. Moreover, smartphone apps become increasingly popular and is likely relevant to provide timely information across development to inform parents about child feeding practices but their effect is seldom evaluated. This randomized controlled trial (RCT) aims to investigate whether the provision of guidelines through an app and a brochure (vs. brochure alone, usual service) results in healthier parental feeding p…