Search results for " metabolites"

Chromatin remodeling redulation by small molecules and metabolites

2010

Grapevine and wine metabolomics-based guidelines for fair data and metadata management

2021

In the era of big and omics data, good organization, management, and description of experimental data are crucial for achieving high-quality datasets. This, in turn, is essential for the export of robust results, to publish reliable papers, make data more easily available, and unlock the huge potential of data reuse. Lately, more and more journals now require authors to share data and metadata according to the FAIR (Findable, Accessible, Interoperable, Reusable) principles. This work aims to provide a step-by-step guideline for the FAIR data and metadata management specific to grapevine and wine science. In detail, the guidelines include recommendations for the organization of data and meta…

Application of Cell Culture for the Production of Bioactive Compounds from Sponges:  Synthesis of Avarol by Primmorphs from Dysidea avara

2000

Among all metazoan phyla, sponges are known to produce the largest number of bioactive compounds. However, until now, only one compound, arabinofuranosyladenine, has been approved for application in humans. One major obstacle is the limited availability of larger quantities of defined sponge starting material. Recently, we introduced the in vitro culture of primmorphs from Suberites domuncula, which contain proliferating cells. Now we have established the primmorph culture also from the marine sponge Dysidea avara and demonstrate that this special form of sponge cell aggregates produces avarol, a sesquiterpenoid hydroquinone, known to display strong cytostatic activity especially against ma…

Ocena aktywności przeciwdrobnoustrojowej ekstraktu roślinnego z Dionaea muscipula

2018

Rośliny owadożerne są przedmiotem badań, nie tylko ze względu na charakterystyczny dla nich sposób pobierania składników odżywczych z otoczenia, ale również z powodu ich pożądanego oddziaływania na mikroorganizmy. W piśmiennictwie znajdujemy potwierdzenie, że ekstrakty z roślin owadożernych mają działanie nie tylko bakteriostatyczne, ale również bakteriobójcze i grzybobójcze. Do badań wybrano jednego z przedstawicieli roślin owadożernych, Dionaea muscipula J. Ellis (Muchołówka amerykańska), w celu określenia ich aktywności biologicznej. Materiałem do badań były odpowiednio przygotowanie ekstrakty z dwóch odmian Dionaea muscipula: Regular Form oraz Red Green. Hodowle prowadzono w warunkach ś…

Perspiration versus saliva--basic aspects concerning their use in roadside drug testing.

1999

Various aspects concerning the practical application and forensic interpretation of data obtained by saliva drug testing and drug monitoring from the skin surface are discussed. Basic information on the composition of saliva and skin secretions and their particular transport mechanisms, as far as known, are given. For drugs of abuse secretion into saliva is suggested to be by passive diffusion and to depend on lipid solubility, pKa, plasma protein binding and on the pH of saliva. Drug molecules from blood are considered to reach the skin surface by various routes such as by sweat and sebum as well as by inter- and/or transcellular diffusion. The role of the stratum corneum as a temporary dr…

Chemical Constituents and Biological Properties of Genus Doronicum (Asteraceae)

2021

The genus Doronicum, belonging to tribe Senecioneae (Fam. Asteraceae), is found mainly in the Asia, Europe and North Africa. This genus of plant has always been used in traditional medicinal treatments due to the many biological properties shown such as killing parasitic worms and for relieving constipation, as well as to improve heart health, to alleviate pain and inflammation, to treat insect bites, etc. According to the World Flora the genus Doronicum contains 39 subordinate taxa.[1-3] The purpose of this article, which covers data published from 1970 to 2021 with more than 110 articles, aims to carry out a complete and critical review of the Doronicum genus, examining traditional uses a…

Histological Change in Cucumber Tissue and Cellulase Activity of Plectosphaerella melonis Strain 502

2022

In the last ten years, many countries around the world recorded a new disease of the Cucurbitaceae, the agent of which was P. melonis. The ability of P. melonis 502 to form intracellular mycelium in the epidermal and parenchymal tissues of roots was shown. Leading tissues (xylem and phloem) did not colonize, which indicates the impossibility of plant vessel clogging and shows the fungus’s biochemical effects on plants, which causes the process of pathogenesis. P. melonis 502 is able to develop in a wide range of pH values, while the pH-optimum is 8.5. P. melonis 502 is able to adjust the pH of the medium to the optimal value—8.5. We also showed that cellulase enzyme synthesis de…

Antimicrobial and Insecticidal: Cyclic Lipopeptides and Hydrogen Cyanide Produced by Plant-Beneficial Pseudomonas Strains CHA0, CMR12a, and PCL1391 C…

2017

Particular groups of plant-beneficial fluorescent pseudomonads are not only root colonizers that provide plant disease suppression, but in addition are able to infect and kill insect larvae. The mechanisms by which the bacteria manage to infest this alternative host, to overcome its immune system, and to ultimately kill the insect are still largely unknown. However, the investigation of the few virulence factors discovered so far, points to a highly multifactorial nature of insecticidal activity. Antimicrobial compounds produced by fluorescent pseudomonads are effective weapons against a vast diversity of organisms such as fungi, oomycetes, nematodes, and protozoa. Here, we investigated whe…

Substantial Fat Loss in Physique Competitors Is Characterized by Increased Levels of Bile Acids, Very-Long Chain Fatty Acids, and Oxylipins.

2022

Funder: Finnish Foundation for Cardiovascular Research

Induction of 2-hydroxycatecholestrogens O-methylation: A missing puzzle piece in diagnostics and treatment of lung cancer

2022

Lung cancer is one of the most common cancers worldwide, causing nearly one million deaths each year. Herein, we present the effect of 2-methoxyestradiol (2-ME), the endogenous metabolite of 17β-estradiol (E2), on non-small cell lung cancer (NSCLC) cells. We observed that 2-ME reduced the viability of lung adenocarcinoma in two-dimensional (2D) and three-dimensional (3D) spheroidal A549 cell culture models. Molecular modeling was carried out aiming to visualize amino acid residues within binding pockets of the acyl-protein thioesterases, namely 1 (APT1) and 2 (APT2), and thus to identify which ones were more likely involved in the interaction with 2-ME. Our findings suggest that 2-ME acts a…