Search results for " microbiologia"
showing 10 items of 907 documents
From microbial proteomics to synthetic biology: Amycolatopsis balhimycina case
2012
Actinomycetes, filamentous Gram-positive bacteria, are usually exploited as bio-farms naturally producing a wide range of small biologically active metabolites, such as antibiotics, extensively used in medicine, food-industry, chemistry and bio-remediation strategies. The development of high throughput technologies, like proteomics, allows functional genomic studies aimed at shedding light on molecular mechanisms controlling the production of useful compounds and macromolecules. Differential proteomic analyses, performed by using Two Dimensional PolyAcrylamide Gel Electrophoresis (2D-PAGE) coupled to mass spectrometry (MS) procedures, revealed novel links between balhimycin production (a va…
Characterisation of the microflora contaminating the wooden vats used for traditional Sicilian cheese production
2015
Traditional Sicilian cheese productions are carried out employing traditional wooden vats, called <em>tina.</em> Many studies have highlighted the beneficial role of wooden dairy equipment by contributing to enriching the milk microflora and improving the acidification processes. The present work was undertaken to evaluate the safety of the wooden vats used to coagulate milk. To this purpose, the different microbial populations hosted onto the internal surfaces of the vats used to produce two different stretched cheeses, namely <em>Caciocavallo Palermitano</em> and <em>Vastedda della valle del Belìce DOP</em>, were investigated for the presence of spoilag…
Microbiological Characteristics of Wild Edible Mushrooms and Effect of Temperature during Storage of Morchella conica
2019
Background: The continuous worldwide increase of consumption of fresh mushrooms has registered in the recent years. The major goal of this study was to determine the microbiological characteristics of wild edible mushrooms and effect of temperature during storage of Morchella conica.
 Methods: Wild mushrooms of the species Boletus edulis, Cantharellus cibarius, and Leccinum aurantiacum were collected in a mixed forest of Picea abies, Betula pendula, and Pinus sylvestris located in Tartu territory, Estonia. Faecal indicators, potential pathogens, spoilage bacteria, and microfungi (yeasts and moulds) were evaluated. M. conica was microbiologically investigated for 24 days under different…
I lipidi ed il loro metabolismo
2016
Gordonia sp. SoCg alkB gene confers the ability to degrade and use n-alkanes as carbon source in Gram positive bacteria
2010
Gordonia sp. SoCg, a Gram positive strain able to grow on long chain n-alkanes1, possess a single copy of alkB2 gene, whose product is required for n-alkane hydroxylation3. An analysis of alkB flanking regions revealed five ORFs which were designed as orf1, rubA3, rubA4, rubB and alkU, according to the sequence 14 homology with that of known alk clusters3. In G. sp. SoCg the transcription of these genes was induced by long-chain and solid n-alkanes as revealed by quantitative RT-PCR, and the essential role of alkB in nalkane degradation was demonstrated by the construction of an alkB disruption mutant strain3. The SoCg alkB gene was successfully expressed in Streptomyces coelicolor M145 (M1…
Procarioti dalle mille risorse: nuove scoperte sui metabolismi batterici che utilizzano la luce
2013
I batteri fototrofi costituiscono un ampio ed eterogeneo gruppo di procarioti ampiamente distribuiti nel plancton marino ed i loro processi basati sulla luce sostengono e controllano il flusso di energia negli oceani. Recenti scoperte di nuovi batteri includono almeno tre modalità di fotosintesi: la fotosintesi aerobica ossigenica (tipica dei cianobatteri e simile a quella condotta dalle piante), la fotosintesi anaerobica anossigenica e la fotosintesi aerobica anossigenica (entrambe esclusive dei procarioti). Altri importanti processi basati sulla luce utilizzano le rodopsine, contribuendo così al metabolismo energetico cellulare. La fotosintesi ossigenica “pura” sembra più l’eccezione che …
La macerazione post-fermentativa prolungata come strategia innovativa per il miglioramento dei vini rossi
2018
Lo scopo del presente studio è stato quello di valutare l’influenza della macerazione post-fermentativa, estesa a 90 giorni, sulla composizione microbica e chimica del vino rosso “Aglianico di Taurasi”. Le analisi microbiologiche hanno messo in evidenza la presenza di popolazioni di lieviti e batteri lattici, vitali e metabolicamente attivi, durante l’intero periodo di macerazione post-fermentativa. Durante il processo di macerazione, la specie principalmente riscontrata era rappresentata da Saccharomyces cerevisiae, mentre al 60° giorno, è stata rilevata una concentrazione simile di Debaromyces carsonii e Zygosaccharomyces bisporus. Tuttavia, è stata osservata una bassa diversità di specie…
CHEMICAL CHARACTERIZATION AND BIOLOGICAL PROPERTIES OF MACROALGAE EXTRACTS
2023
Marine species are a rich source of bioactive molecules and among them, it is known that marine algae produce different secondary metabolites for which different biological activities such as: immunomodulatory (Raposo et al., 2016), antioxidant (Fisch et al., 2003) and antimicrobial (Pinteus et al., 2015) were demostrated. The aim of this study was chemical charac- terize the extracts of three macroalgae species: Carpodesmia crinite (Duby, Orellana & Sansón, 2019), Carpodesmia brachy- carpa (J. Agardh, Orellana & Sansón 2019, WoRMS, 2023), Ericaria brachycarpa (J. Agardh, Molinari & Guiry, 2020), Asparagopsis taxiformis (Delile) Trevisan 1845) to evaluate also their biological a…
Antifouling Systems Based on a Polyhedral Oligomeric Silsesquioxane-Based Hexyl Imidazolium Salt Adsorbed on Copper Nanoparticles Supported on Titania
2023
The reaction of octakis(3-chloropropyl)octasilsesquioxane with four equivalents of 1-hexylimidazole or 1-decylimidazole gave two products labelled as HQ-POSS (hexyl-imidazolium quaternized POSS) and DQ-POSS (decyl-imidazolium quaternized POSS) as regioisomer mixtures. An investigation of the biological activity of these two compounds revealed the higher antimicrobial performances of HQ-POSS against Gram-positive and Gram-negative microorganisms, proving its broad-spectrum activity. Due to its very viscous nature, HQ-POSS was adsorbed in variable amounts on the surface of biologically active oxides to gain advantages regarding the expendability of such formulations from an applicative perspe…
The early plastisphere of biodegradable and non-biodegradable plastic films in marine environment
2019
Introduction Plastic items are the most common form of marine debris and plastic accumulation in marine ecosystems is a serious environmental issue. One possible solution to reduce the amount of synthetic polymers released in the environment is to substitute them with bio-based polymers. When released in marine environments, plastics are readily colonized by biofilm-forming microorganisms, the so called “plastisphere community”, which may include pathogenic, invasive and plastic-degrading species; the composition and influence of the plastisphere on the fate of plastic is largely unknown. The aim of this study is to investigate the early biofilm formation and the structure and diversity of …