Search results for " microbiologia"

Bioremediation of chronically oil-polluted marine sediment using beats of hydrocarbons-degrading bacteria

2015

Bioremediation is a good kind of "green biotechnology" based on the microorganisms ability to degrade hydrocarbons fractions. In this work sediment samples were collected from the industrial harbor of Priolo Gargallo (Augusta, Syracuse, Sicily - Italy), a chronically polluted area. Enrichment cultures and microbial isolation were performed. Amoung 258 bacteria and 5 consortia isolated, strain S1 (Alcanivorax borkumensis, 98%) and two consortia (PSO and PSM) showed degradation rates of ~98% for linear and polycyclic aromatic hydrocarbons (PHAs) after 10 days of incubation (25±1°C, shaking 100 g). Taxonomic analysis (16S clone libraries) of consortia showed as dominant genera hydrocarbonoclas…

Biotechnological innovations in fermentation process of brewing and honey-based beverages industry

2022

La ricerca di microrganismi fermentanti in grado di migliorare le proprietà microbiologiche, fisiche, chimiche, sensoriali e organolettiche delle bevande alcoliche fermentate come vino, birra, idromele, sidro, è considerata ad oggi un punto chiave per lo sviluppo del settore. Negli ultimi anni, l'aumento della domanda al consumo di nuove bevande fermentate ha causato un enorme bisogno di tecnologie di produzione innovative al fine di ottenere diverse tipologie di prodotti caratterizzati da proprietà sensoriali peculiari. Per raggiungere tale obiettivo, l’impiego di nuovi microrganismi, sia fermentanti che non fermentanti, in grado di conferire al prodotto caratteristiche organolettiche pecu…

Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa…

2014

The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiof…

Essential Oil Composition of Alluaudia procera and in Vitro Biological Activity on Two Drug-Resistant Models

2019

Drug resistance is a major obstacle in antibiotic and antitumor chemotherapy. In response to the necessity to find new therapeutic strategies, plant secondary metabolites including essential oils (EOs) may represent one of the best sources. EOs in plants act as constitutive defenses against biotic and abiotic stress, and they play an important role in the pharmacology for their low toxicity, good pharmacokinetic and multitarget activity. In this context, natural products such as EOs are one of the most important sources of drugs used in pharmaceutical therapeutics. The aim of this paper was to identify the chemical composition of the essential oil of Alluaudia procera leaves, obtained by hy…

Microbial technologies for the discovery of novel bioactive metabolites

2002

Soil microbes represent an important source of biologically active compounds. These molecules present original and unexpected structure and are selective inhibitors of their molecular targets. At Biosearch Italia, discovery of new bioactive molecules is mostly carried out through the exploitation of a proprietary strain collection of over 50000 strains, mostly unusual genera of actinomycetes and uncommon filamentous fungi. A critical element in a drug discovery based on microbial extracts is the isolation of unexploited groups of microorganisms that are at the same time good producers of secondary metabolites. Molecular genetics can assist in these efforts. We will review the development an…

Pathogen safety of long-term treatments for bleeding disorders: (un)predictable risks and evolving threats.

2013

Substantial improvements in the safety of blood and plasma products for the management of bleeding disorders have been achieved in recent decades. This has led some clinicians to believe that the infectious threat is over and that inhibitor formation is the foremost complication of hemophilia therapy. On the contrary, elimination of all microbes from blood is difficult, potentially impossible, and there are always threats from emerging pathogens. The risk of infection transmission is also increasing due to greater exposure to products, increasing prophylaxis and high-dose regimens for immune tolerance, and longevity of hemophilia patients. Current products can be considered "reasonably safe…

ENTERAL NUTRITION SUPPORT TO TREAT MALNUTRITION IN INFLAMMATORY BOWEL DISEASE

2015

Malnutrition is a common consequence of inflammatory bowel disease (IBD). Diet has an important role in the management of IBD, as it prevents and corrects malnutrition. It is well known that diet may be implicated in the aethiology of IBD and that it plays a central role in the pathogenesis of gastrointestinal tract disease. Often oral nutrition alone is not sufficient in the pathogenesis of gastrointestinal tract disease. Often oral nutrition alone is not sufficient in the management of IBD patients, especially in children or elederly, and must be combined with oral supplementation or replaced with tube enteral nutrition. In this review, we describe several different approaches to enteral …

HCV CLEARANCE AFTER PEG IFN PLUS RBV IMPROVES THE COURSE OF HCV CIRRHOSIS REGARDLESS OF PORTAL HYPERTENSION

2009

Longitudinal metagenomics

2018

Culture-independent approaches are revolutionizing biology. Whether in a clinical or in an environmental sample, metagenomics can reveal which microorganisms exist and what they actually do. Metagenomic studies have unveiled more microbial diversity in a few years than traditional microbiology in centuries. From the top branches down to the roots, its discoveries are reshaping the tree of life dramatically. Metagenomics is a powerful tool for the study of microbial communities, but it requires equally powerful methods of analysis. Current challenges in the analysis of metagenomic data include the accurate comparison of samples, the estimation of the uncertainty in the results, and the effec…

Profiling microbial communities in hyperalkaline waters of the Kizildag ophiolite complex (Turkey).

2016

It has recently been hypothesized that life on Earth could have been originated in hyperalkaline waters related to serpentinization of ophiolitic rocks, despite their extreme conditions (high pH and very low levels of nutrients). Five hyperalkaline springs of the Kizildag ophiolite complex (Turkey) were characterized. The dominant gases are either H2, CH4 or N2 . Bacterial diversity, analysed by RISA (Ribosomal Intergenic Spacer Analysis) revealed different profles for each spring. Sequencing of excised DNA bands allowed to identify the presence of Bacillus, Ralstonia, Pseudoalteromonas., Ureibacillus, Alicycliphilus, Anaerococcus. 16S ribosomal DNA sequencing by Illumina is in progress. Th…