Search results for " miglioramento"
showing 10 items of 219 documents
ANALYSIS OF MICROSATELLITE MARKERS IN SICILIAN GOATS BREED FOR TRACEABILITY OF GIRGENTANA TYPICAL DAIRY PRODUCTS
2013
The establishment of useful analytical methods able to ensure the origin of the products, including the breed used, are important in maintaining the reliability of these products in order to develop a market segment. Traceability, obtained by molecular analysis, could be a reliable proposal for the authentication and valorization of animal products. In Sicily, the three most important dairy goat breeds are Girgentana (GR), Maltese (ML) and Derivata di Siria (DS). The GR is an endangered autochthonous goat breed. Preservation of endangered breeds could be achieved by establishing economic reasons for their survival. The aim of this work was to verify the use of microsatellite markers to asse…
Microbiological and physicochemical profile of sugar added ewe’s Ricotta during storage
2022
The microbiological and physicochemical traits of fresh ewe’s Ricotta made with (40% w/w) and without sucrose were investigated. Sugar added Ricotta (SAR) andcontrol Ricotta (CTR) were packaged and stored at 4°C for 90 d. Salmonella spp., Listeria monocytogenes,Escherichia coli, coagulase positive staphylococci, Enterobacteriaceae, total coliforms, Pseudomonas, yeasts, moulds and clostridia were undetectable during the entire monitoring. Total mesophilic and psychrotrophic microorganisms and mesophilic cocci lactic acid bacteria increased over time,but their development was limited in SAR production. The acidificationwas observed only for CTR. Water activity was consistently low(0.897 on av…
Genetic structure and assignment test in five sheep breeds reared in Sicily using microsatellites
2011
A total of 331 individuals from five sheep breeds reared in Sicily (Barbaresca (BAR, n=57), Comisana (COM, n=65), Pinzirita (PIN, n=75), Sarda (SAR, n=64), and Valle del Belice (VdB, n=70)) were analysed to investigate genetic structure and perform an assignment test. A panel of 20 microsatellite markers was used, and a total of 259 alleles were observed. The polymorphic information content (PIC) all over loci was equal to 0.757, showing that the microsatellites panel used was highly informative. Estimates of observed heterozygosity ranged from 0.655 in the BAR breed to 0.751 in the COM breed. The low value of genetic differentiation among breeds (FST=0.048) may indicate that these breeds a…
Use of microsatellite markers for genetic traceability of Girgentana dairy products
2015
Genetic traceability is based on the identification of both animals and their products through the study of DNA. With the goal of developing a genetic traceability system for dairy products, the aim of this study was to identify specific microsatellite markers able to discriminate among the most important Sicilian dairy goat breeds, in order to detect possible adulteration in Girgentana dairy products. A total of 20 microsatellite markers were analyzed on a total of 338 individual samples from Girgentana (GIR), Maltese (MAL) and Derivate di Siria (DdS) goat breeds. The first step was to identify breed specific microsatellite markers that can be used for the traceability of dairy products. A…
Survey of milk protein polymorphism in the "Bovina Rossa Siciliana"
2005
The “Rossa Siciliana” is an autochthonous small Sicilian dairy cattle population, accounting for 5-7,000 individuals.This breed is characterized by high ability to live on poor pasture lands, moderate milk production, and traditionally linked to cheese production. Aim of this work was to investigate milk protein polymorphisms in this population. A total of 62 individual milk samples were collected from 19 extensive farms spread in the “Parco dei Nebrodi” area (Messina). All samples were analyzed by isoelectrofocusing (IEF) with pH range 2.5-6. High variability was found at the CSN1S1, CSN2, CSN3, LGB and LALBA loci, while the CSN1S2 locus was monomorphic for the A allele. The allele frequen…
Genome-wide scan for Runs of Homozygosity in Valle del Belice sheep
2017
The current availability of very large numbers of single nucleotide polymorphisms (SNPs) throughout the genome makes these markers particularly suitable for the detection of genomic regions where a reduction in heterozygosity occurred and offers new opportunities to improve the accuracy of inbreeding (F) estimates. Runs of homozygosity (ROH) are contiguous lengths of homozygous segments of the genome where the two haplotypes inherited from the parents are identical. Here, we investigated the occurrence and the distribution of ROH in medium-density SNP genotypes (~ 50 000) in order to characterize autozygosity in 512 individuals of Valle del Belice sheep and identify the regions of the genom…
Demographic, Genealogical and morphological characterization of Pantelleria donkey
2004
A demographic, genetic and morphological evaluation on Pantelleria donkey's population was carried out to give a contribution for the exploitation of this old donkey breed. Genealogical data were analysed by the software Minbreed v 1.0. Pedigree is composed by 4 generations. The inbreeding of the population (Fwhole population = 0.4 %) resulted very low than the expectations; therefore, the inbreeding coefficients resulted low in the males F male, = 0.9 % and in the females Ffemale = 0.0 %. On the other hand, the mean relationship among the 378 couplings resulted very high a1j = 15,1 %. The morphological study was carried out on 20 subjects. For each animal the most important morphologic…
Genetic and protein polymorphism at CSN1S1 locus in two goat breeds.
2006
Genetic polymorphisms of casein have received a considerable research effort for many years because of its potential effect on milk composition. Milk composition, specifically protein content, differs quantitatively and qualitatively among species and also among breeds and individuals. Sicilian goat breeds seem to be an interesting model since they have never been under a selection program, and therefore may carry unique casein polymorphisms. Moreover, the importance of goat milk in infant diets is growing probably because goat milk in some cases is less allergenic than cow milk. It is important to evaluate the genetic polymorphisms at the CSN1S1 locus in the populations of interest, like t…
Cellule somatiche in latte ovino: analisi e applicazione delle curve ROC
2013
Negli ovini da latte le mastiti costituiscono una delle principali cause di perdite economiche per la ridotta produzione e soprattutto per la scarsa qualità tecnologica del latte. Diversi studi hanno dimostrato che il contenuto in cellule somatiche (SCC) del latte può essere impiegato sia come metodo diagnostico sia come criterio di selezione nei programmi di miglioramento genetico per la resistenza alle mastiti. Obiettivo di questo lavoro è stato applicare la metodologia delle Receiver Operating Characteristics curves (ROC) per valutare la capacità diagnostica del SCC nel discriminare le mammelle infette da quelle sane e per individuare un valore soglia discriminante in ovini da latte. In …
Distribution of functional variants within Runs of Homozygosity in four Italian cattle breeds
2017
Several methods have been used to identify genomic regions subjected to selective sweeps. An alternative method can make use of runs of homozygosity (ROH), defined as stretches of homozygous regions in a genome. The aim of the present study was to detect selective sweeps using ROH in four cattle breeds. Individuals of Cinisara (71), Modicana (72),Reggiana (168) and Italian Holstein (96) were genotyped with the Illumina Bovine50SNP v2 BeadChip. To identify genomic regions that were most commonly associated with ROH within each breed, the percentage of occurrences of a single nucleotide polymorphism (SNP) in ROH was calculated across animals. The genomic regions most commonly associated with …