Search results for " miglioramento"

Tracking Zebu Introgression in Mediterranean Cattle Breeds

2017

A recent work investigating genetic origin, admixture and population history of primitive European cattle highlighted, by using genome-wide single nucleotide polymorphisms, zebu gene flow in the Balkan and Italian Podolic cattle populations. Haemoglobin protein polymorphism analysis in Italian breeds highlighted the presence of zebuine markers in both Italian Podolic and Alpine Grey cattle. Based on the above evidences, we here specifically look for genomic regions of zebuine ancestry in a different dataset of 50K genotypes from Mediterranean breeds including 29 Marismena (Spain); 30 Bazadaise and 30 Gasconne (France); 24 Alpine Grey, 97 Piemontese, 51 Chianina, 5 Marchigiana, 121 Romagnola…

Genetic diversity and phylogenetic relationships among four breeds reared in Sicily using β-lactoglobulin promoter region polymorphisms

2011

The aim of this work was to analyze the genetic diversity and the phylogenetic relationships among the Valle del Belice (VDB) breed and the other sheep breeds considered as ancestors, using -lactoglobulin (BLG) promoter region polymorphisms. The BLG promoter region of 50 randomly chosen animals from the four breeds (VDB n=20; Comisana (COM) n=10; Pinzirita (PIN) n=10; Sarda (SAR) n=10) were sequenced and aligned, and results showed the presence of 36 single nucleotide polymorphisms (SNPs) and one deletion. The number of polymorphic sites identified showed high variability within breeds. Nucleotide (π) and haplotype (Hd) diversity were estimated with DnaSP software, whereas PHASE software w…

Analysis of Illumina BovineSNP50 BeadChip data in different Italian cattle breeds to identify useful markers for breed authentication of dairy and be…

2013

The identification of the breed of origin of cattle derived products has recently assumed particular relevance since the increasing interest in marketing mono-breed labelled cheese and beef. These products are usually sold at a higher price creating financial incentives to substituting them with lower value products coming from undifferentiated or common breeds or crossbred animals. Frauds can damage these valuable niche markets that, indirectly, may help to preserve genetic diversity through the economic incentive on raising local breeds, derived by the high value products, that are usually less productive. DNA analysis can help to identify fraudulent behaviour as we already demonstrated f…

Weighted Single-Step Genome-Wide Association Study Uncovers Known and Novel Candidate Genomic Regions for Milk Production Traits and Somatic Cell Sco…

2022

The objective of this study was to uncover genomic regions explaining a substantial proportion of the genetic variance in milk production traits and somatic cell score in a Valle del Belice dairy sheep. Weighted single-step genome-wide association studies (WssGWAS) were conducted for milk yield (MY), fat yield (FY), fat percentage (FAT%), protein yield (PY), protein percentage (PROT%), and somatic cell score (SCS). In addition, our aim was also to identify candidate genes within genomic regions that explained the highest proportions of genetic variance. Overall, the full pedigree consists of 5534 animals, of which 1813 ewes had milk data (15,008 records), and 481 ewes were genotyped with a …

Indagine preliminare sul polimorfismo lattoproteico in diverse popolazioni caprine allevate in Sicilia.

2004

La consistenza del patrimonio caprino in Italia ha subito negliiltimi anni un aumento considerevole. Significativo è stato l’incremento del 6,6% della produzione di latte tra il 2000 e il 2002. Il recente sviluppo dell’allevamento caprino è dovuto in gran parte al crescente interesse dei consumatoru sia verso i prodotti caseari tipici sia verso il consumo diretto del latte. Le caseina caprine sono caratterizzate da un elevato polimorfismo genetico, sia a livello qualitativo che quantitativo, diventato oggetto di un generale interesse scientifico per le importanti relazioni con la qualità e le caratteristiche nutrizionali e tecnologiche del latte. Il patrimonio caprino siciliano è rappresent…

Caratterizzazione genetica mediante microsatelliti di una popolazione caprina siciliana

2013

I microsatelliti sono ad oggi i marcatori molecolari maggiormente utilizzati per la caratterizzazione genetica nei caprini. Lo scopo del presente lavoro è stato quello di caratterizzare la struttura genetica della capra Mascaruna per verificare se può essere definita come una popolazione. L’analisi è stata condotta utilizzando un pannello di 18 microsatelliti. Il DNA è stato estratto da 60 individui di cui 20 Mascaruna (MAS), 20 Girgentana (GIR) e 20 animali derivanti da diversi incroci (MIX). Un totale di 148 alleli sono stati osservati di cui 106 in GIR, 107 in MAS e 129 in MIX; il valore del PIC è di 0,69 e tutti i marcatori hanno mostrato un numero di alleli superiori a 4. Valori più al…

Cellule somatiche ed isolamento di agenti mastidogeni in ovini di razza Valle del Belice

2013

Il conteggio delle cellule somatiche nel latte ovino si è dimostrato un valido strumento per la determinazione dello stato sanitario della mammella. Oggetto del presente studio è stato valutare eventuali relazioni fra il contenuto in cellule somatiche (SCC) e le infezioni mammarie (IMI) negli ovini. L’indagine è stata condotta nel periodo 2006-2012 su n.14072 campioni di latte individuale. In base al tenore i campioni sono state inseriti in 4 classi: 300.000; da 301.000 a 500.000; da 501.000 a 1.000.000 e ≥1.001.000 cellule/ml. Per l’isolamento colturale sono stati seminati 10 μl di latte su piastre di Agar Sangue incubate per 24-48 h a 37 °C in aerofilia e, se necessario, in microaerof…

Stima delle componenti della (co)varianza per il contenuto in cellule (CCS) in pecore di razza Valle del Belice

2004

Trend in dairy sheep BLG genotype found with repeatability test-day model.

2007

A total of 19.207 test-days, collected on 4 farms from 1999-2006 and belonging to 1109 Valle del Belice dairy sheep were analyzed with a repeatability model. After strict outlier analysis 17.747 records were retained. Animals were reared in an extensive system resulting in large environmental influences. However, significant genetic variation was detected in production traits (milk production mean=1336 stdg=93 g/d, fat+protein 167 stdg=12 g/d). Heritability was only 3% while the interaction year by month of test-day explained 27% of the variation. A protein and DNA analysis program was initiated in order to facilitate selection to maintain these flocks under these conditions. In total 427 a…

Parentage verification of Valle del Belice dairy sheep using microsatellite markers

2008

The objectives of the present work were to evaluate polymorphism of microsatellite markers, develop a parentage test and estimate misidentification rate on Valle del Belice Sicilian dairy sheep breed. Polymorphism was evaluated from 184 randomly selected animals from 5 different flocks. A total of 21 microsatellite markers including CSRD247, ILST011, McM527, APO010, INRA132, DU194351, DU323541, FCB128, McM16, OarCP49, ILST087, DU223896, ILST005, TCRVB06, SPS113A, INRA063A, SPS115, DU206192, DU216028, BM827 and McM54 were amplified using three PCR reactions and fragment lengths determined in a single multiplex electrophoresis run. Markers were easily genotyped, very polymorphic and in Hardy-…