Search results for " mito."
showing 10 items of 892 documents
Manipulating mtDNA in vivo reprograms metabolism via novel response mechanisms.
2019
Mitochondria have been increasingly recognized as a central regulatory nexus for multiple metabolic pathways, in addition to ATP production via oxidative phosphorylation (OXPHOS). Here we show that inducing mitochondrial DNA (mtDNA) stress in Drosophila using a mitochondrially-targeted Type I restriction endonuclease (mtEcoBI) results in unexpected metabolic reprogramming in adult flies, distinct from effects on OXPHOS. Carbohydrate utilization was repressed, with catabolism shifted towards lipid oxidation, accompanied by elevated serine synthesis. Cleavage and translocation, the two modes of mtEcoBI action, repressed carbohydrate rmetabolism via two different mechanisms. DNA cleavage activ…
A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice
2020
We previously observed an unexpected fivefold (35 vs. 200 days) difference in the survival of respiratory chain complex III (CIII) deficient Bcs1lp.S78G mice between two congenic backgrounds. Here, we identify a spontaneous homoplasmic mtDNA variant (m.G14904A, mt-Cybp.D254N), affecting the CIII subunit cytochrome b (MT-CYB), in the background with short survival. We utilize maternal inheritance of mtDNA to confirm this as the causative variant and show that it further decreases the low CIII activity in Bcs1lp.S78G tissues to below survival threshold by 35 days of age. Molecular dynamics simulations predict D254N to restrict the flexibility of MT-CYB ef loop, potentially affecting RISP dyna…
The Many Souls of Dallapiccola's "Ulysses"
2009
L'"Ulisse" di Dallapiccola somma esperienze letterarie molto diverse tra loro. Somma inoltre alla rivisitazione di un mito complesso e stratificato, l'ambizione di ridare vita a un genere, quello operistico, che sembra aver concluso il suo percorso. Somma infine nel protagonista le caratteristiche dell'astuto personaggio di Omero e di quello curioso e inappagato di Dante. Ma non solo: l'Ulisse di Dallapiccola aggiunge a queste il dubbio, l'incertezza, l'interrogare e l'interrogarsi che ne fanno un cittadino della modernità. E in questa veste, passando per l'immagine di Cristoforo Colombo, impersona colui che fu capace di mostrare al compositore "un altro Nord", il suo grande maestro, Arnold…
Cytotoxic Activity of Organotin(IV) Derivatives with Triazolopyrimidine Containing Exocyclic Oxygen Atoms
2020
In this study cytotoxicity of organotin(IV) compounds with 1,2,4-triazolo[1,5-a]pyrimidines, Me3Sn(5tpO) (1), n-Bu3Sn(5tpO) (2), Me3Sn(mtpO) (3), n-Bu3Sn(mtpO) (4), n-Bu3Sn(HtpO2) (5), Ph3Sn(HtpO2) (6) where 5HtpO = 4,5-dihydro-5-oxo-[1,2,4]triazolo-[1,5-a]pyrimidine, HmtpO = 4,7-dihydro-5-methyl-7-oxo-[1,2,4]triazolo-[1,5-a]pyrimidine, and H2tpO2 = 4,5,6,7-tetrahydro-5,7- dioxo-[1,2,4]triazolo-[1,5-a]-pyrimidine, was assessed on three different human tumor cell lines: HCT-116 (colorectal carcinoma), HepG2 (hepatocarcinoma) and MCF-7 (breast cancer). While 1 and 3 were inactive, compounds 2, 4, 5 and 6 inhibited the growth of the three tumor cell lines with IC50 values in the submicromolar …
Введение въ исторію бородатыхъ карликообразныхъ божествъ въ Египтѣ
1913
Kopsavilkums krievu valodā.
An extension of the Burrows-Wheeler Transform and applications to sequence comparison and data compression
2005
We introduce a generalization of the Burrows-Wheeler Transform (BWT) that can be applied to a multiset of words. The extended transformation, denoted by E, is reversible, but, differently from BWT, it is also surjective. The E transformation allows to give a definition of distance between two sequences, that we apply here to the problem of the whole mitochondrial genome phylogeny. Moreover we give some consideration about compressing a set of words by using the E transformation as preprocessing.
La Antígona de Eurípides y el P. Oxy. 3317
2019
Eurípides trató el Ciclo Tebano en Fenicias y Suplicantes, obras que hemos conservado, y en Antígona, de la que sólo conservamos unos escasos fragmentos, al igual que de su Edipo; en estas dos últimas, por lo que podemos saber por los fragmentos y las referencias, se observa un distan- ciamiento en el tratamiento dramático de los mitos que observamos en Sófocles. Precisamente ésta es una de las pocas unanimidades que existen respecto a Antígona de Eurípides, que la joven heroína no compartía el destino de la sofoclea y, lejos de morir, se unía a Hemón y tenía con él un hijo. Determinar si esto era el punto final del drama o el arranque del mismo sigue siendo objeto de debate. El problema de…
Lungo il filo di Arianna. Voci del mito al femminile
2012
Le varianti del mito che hanno Arianna come protagonista sono molte e tutte cariche di significato. Il lavoro ha come protagoniste donne del mito, attraverso le voci di Gea, Pandora, Andromaca, Elena...La forma del monologo consente di "dare vita" al travaglio dell'animo femminile che, attraverso un processo di consapevolezza, rende reali situazioni vissute nella dimensione fantastica del mito. abstractita
Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway
2012
Dryofragin is a phloroglucinol derivative extracted from Dryopteris fragrans (L.) Schott. In this study, the anticancer activity of dryofragin on human breast cancer MCF-7 cells was investigated. Dryofragin inhibited the growth of MCF-7 cells in a time and concentration-dependent manner. The cell viability was measured using MTT assay. After treatment with dryofragin for 72, 48 and 24 h, the IC₅₀ values were 27.26, 37.51 and 76.10 μM, respectively. Further analyses of DNA fragmentation and Annexin V-PI double-labeling indicated an induction of apoptosis. Dryofragin-treatment MCF-7 cells had a significantly accumulation of reactive oxygen species (ROS), as well as an increased percentage of …
PD‐1‐induced T cell exhaustion is controlled by a Drp1‐dependent mechanism
2022
Programmed cell death‐1 (PD‐1) signaling downregulates the T‐cell response, promoting an exhausted state in tumor‐infiltrating T cells, through mostly unveiled molecular mechanisms. Dynamin‐related protein‐1 (Drp1)‐dependent mitochondrial fission plays a crucial role in sustaining T‐cell motility, proliferation, survival, and glycolytic engagement. Interestingly, such processes are exactly those inhibited by PD‐1 in tumor‐infiltrating T cells. Here, we show that PD‐1pos CD8+ T cells infiltrating an MC38 (murine adenocarcinoma)‐derived murine tumor mass have a downregulated Drp1 activity and more elongated mitochondria compared with PD‐1neg counterparts. Also, PD‐1pos lymphocytic elements in…