Search results for " modifica"
showing 10 items of 817 documents
The importance of physical education and health to be a core subject: Arguments and postures concerning the curricular modifications in Chile
2021
This study is focused on the declarations made by different Chilean institutions regarding the modifications affecting the subject Physical Education and Health (PEH), which were approved by the National Education Council. Purpose: The main aim was to analyse the speech generated and gather data around the curricular modifications in the 3rd and 4th course of the middle education in 2020, specifically the choice of leaving the subject PEH as an elective subject. Design: A qualitative, pragma-dialectic approach was used, focusing on the point of view and main arguments of the institutions on the topic. Results: 23 declarations were reviewed, pertaining 6 to health institutions, 12 to educati…
When the functionalisation comes in useful: ionic liquids with a “sweet” appended moiety demonstrate drastically reduced toxicological effects
2020
The growing number of applications of ionic liquids (ILs) in industry have brought attention to the green credentials of synthesis, as well as their cytotoxicities and ecotoxicities both for their use and accidental leakage into the environment. With the abovementioned properties in mind, we designed a class of ILs with either cations bearing a gluconamide motif and aliphatic side chains or the anion incorporating a gluconic acid (derived from food waste) moiety. An IL with an imidazolium cation with an appended gluconic amide (bearing five hydroxyl groups) moiety was also synthesized for a useful comparison. Different structural features were considered, placing emphasis on the nature and …
Editorial: Zebrafish Epigenetics.
2022
A key area of focus in the field of epigenetics pertains the comprehension of the functional relevance of the epigenetic mechanisms occurring during embryogenesis to shape normal developmental trajectories and adult phenotypes (Atlasi and Stunnenberg, 2017; Skvortsova et al., 2018; Cavalieri, 2021; Marchione et al., 2021). Several lines of evidence highlighted that the small freshwater cyprinid Danio rerio, commonly known as zebrafish, is an excellent vertebrate model for research purposes in the field of epigenetics (Huang et al., 2013; Balasubramanian et al., 2019; Horsfield, 2019; Cavalieri, 2020). The general strengths of zebrafish over concurrent models are well known: ease of husbandr…
The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability.
2008
Abstract Lysyl hydroxylase (LH) isoform 3 is a post-translational enzyme possessing LH, collagen galactosyltransferase (GT) and glucosyltransferase (GGT) activities. We have demonstrated that LH3 is found not only intracellularly, but also on the cell surface and in the extracellular space, suggesting additional functions for LH3. Here we show that the targeted disruption of LH3 by siRNA causes a marked reduction of both glycosyltransferase activities, and the overexpression of LH3 in HT-1080 cells increases hydroxylation of lysyl residues and the subsequent galactosylation and glucosylation of hydroxylysyl residues. These data confirm the multi-functionality of LH3 in cells. Furthermore, t…
Manipulating mtDNA in vivo reprograms metabolism via novel response mechanisms.
2019
Mitochondria have been increasingly recognized as a central regulatory nexus for multiple metabolic pathways, in addition to ATP production via oxidative phosphorylation (OXPHOS). Here we show that inducing mitochondrial DNA (mtDNA) stress in Drosophila using a mitochondrially-targeted Type I restriction endonuclease (mtEcoBI) results in unexpected metabolic reprogramming in adult flies, distinct from effects on OXPHOS. Carbohydrate utilization was repressed, with catabolism shifted towards lipid oxidation, accompanied by elevated serine synthesis. Cleavage and translocation, the two modes of mtEcoBI action, repressed carbohydrate rmetabolism via two different mechanisms. DNA cleavage activ…
Development of stir bar sorptive-dispersive microextraction mediated by magnetic nanoparticles and its analytical application to the determination of…
2014
A novel microextraction technique combining the principles of stir bar sorptive extraction (SBSE) and dispersive micro-solid phase extraction (DμSPE) is presented. The main feature of the method is the use of a neodymium-core stirring bar physically coated with a hydrophobic magnetic nanosorbent. Depending on stirring speed, the magnetic sorbent either acts as a coating material to the stir bar, thus affording extraction alike SBSE, or as a dispersed nanosorbent medium for the collection and extraction of the target analytes, in close analogy to DμSPE. Once the stirring process is finished, the strong magnetic field of the stir bar prevails again and rapidly retrieves the dispersed MNPs. Al…
Multifunctional two-photon active silica-coated Au@MnO Janus particles for selective dual functionalization and imaging.
2014
Monodisperse multifunctional and nontoxic Au@MnO Janus particles with different sizes and morphologies were prepared by a seed-mediated nucleation and growth technique with precise control over domain sizes, surface functionalization, and dye labeling. The metal oxide domain could be coated selectively with a thin silica layer, leaving the metal domain untouched. In particular, size and morphology of the individual (metal and metal oxide) domains could be controlled by adjustment of the synthetic parameters. The SiO2 coating of the oxide domain allows biomolecule conjugation (e.g., antibodies, proteins) in a single step for converting the photoluminescent and superparamagnetic Janus nanopar…
Microwave assisted syntheses of solvent-based colloidal sols of tailored ceria nanoparticles
2009
AbstractMicrowave assisted syntheses of stable solvent based colloidal sols of tailored ceria nanoparticles Olivier Poncelet*, Julien Jouhannaud*, Denis Chaumont** *CEA Liten DTNM/L2T , 17 rue des Martyrs F-38054 Grenoble Cedex9, France **Institut Carnot Bourgogne (ICB), UMR5209 CNRS, Facult� des Sciences Mirande, Universit� de Bourgogne, 9 Av. Alain Savary, BP 47870, F-21078 Dijon Cedex The pressure of environmental laws in many advanced countries becoming more restricting year after year, it is asked to automobile companies to strongly control the carburant consumption of cars that they put on the market and also to eliminate toxic chemicals coming from exhaust emission gases. This is par…
Evaluation of 2,3-epoxypropyl groups and functionalization yield in glycidyl methacrylate monoliths using gas chromatography
2014
Abstract Poly(glycidyl methacrylate- co -ethylene dimethacrylate) (poly(GMA- co -EDMA)) is most frequently used as parent monolith to obtain stationary phases with a variety of surface chemistries for liquid chromatography and capillary electrochromatography. Functionalization is performed by opening the accessible 2,3-epoxypropyl groups of the monolith with a suitable reagent. The number of 2,3-epoxypropyl groups which are accessible before and after the functionalization reaction, and the grafting yield, are important parameters, required both to optimize functionalization and to interpret the chromatographic performance of functionalized monoliths. In this work, a method capable of provi…
Heterobimetallic single-source precursors for MOCVD. Synthesis and characterization of volatile mixed ligand complexes of lanthanides, barium and mag…
1999
Several heterobimetallic complexes of general formula [(M 1 Q) m M 2 (dik) n ], where (M 1 Q) is Cu(salen) or Ni(salen) and M 2 (dik) n is a lanthanide tris(β-diketonate) or an alkali-earth element bis(β-diketonate) with more or less fluorinated diketonato(-) ligands, have been prepared and structurally characterized. Some of them were submitted to thermal and mass spectrometry analyses, and to isothermal vacuum sublimation tests, in view of their potential use as MOCVD single-source precursors for the deposition of heterobimetallic materials. The study stresses upon the influence of the nature of the diketone. of the M 2 cation, and of chemical modification of the salen ligand on the therm…