Search results for " molecular evolution"

showing 2 items of 22 documents

Microbial technologies for the discovery of novel bioactive metabolites

2002

Soil microbes represent an important source of biologically active compounds. These molecules present original and unexpected structure and are selective inhibitors of their molecular targets. At Biosearch Italia, discovery of new bioactive molecules is mostly carried out through the exploitation of a proprietary strain collection of over 50000 strains, mostly unusual genera of actinomycetes and uncommon filamentous fungi. A critical element in a drug discovery based on microbial extracts is the isolation of unexploited groups of microorganisms that are at the same time good producers of secondary metabolites. Molecular genetics can assist in these efforts. We will review the development an…

medicine.medical_specialtyGenetic VectorsBioengineeringComputational biologyBiologySettore BIO/19 - Microbiologia Generalemedicine.disease_causeApplied Microbiology and BiotechnologyStreptomycesGenomePolymerase Chain ReactionMicrobiologySpecies SpecificityMolecular geneticsmedicineGeneEscherichia coliSoil MicrobiologyDrug discoveryGeneral MedicineGene Expression Regulation Bacterialbiology.organism_classificationIsolation (microbiology)ActinobacteriaGenetic VectorDirected Molecular EvolutionSoil microbiologyActinobacteria; Directed Molecular Evolution; Genetic Vectors; Polymerase Chain Reaction; Soil Microbiology; Species Specificity; Gene Expression Regulation BacterialBiotechnology
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Prediction of harmful variants on mitochondrial genes : Test of habitat-dependent and demographic effects in a euryhaline fish

2017

Both effective population size and life history may influence the efficacy of purifying selection, but it remains unclear if the environment affects the accumulation of weakly deleterious nonsynonymous polymorphisms. We hypothesize that the reduced energetic cost of osmoregulation in brackish water habitat may cause relaxation of selective constraints at mitochondrial oxidative phosphorylation (OXPHOS) genes. To test this hypothesis, we analyzed 57 complete mitochondrial genomes of Pungitius pungitius collected from brackish and freshwater habitats. Based on inter- and intraspecific comparisons, we estimated that 84% and 68% of the nonsynonymous polymorphisms in the freshwater and brackish …

mtDNAnearly neutral theory of molecular evolutionHypothesesgenetic loadselective constraintadaptation
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