Search results for " mouse"
showing 10 items of 343 documents
C1 Inhibitor-C1¯sComplexes Are Internalized and Degraded by the Low Density Lipoprotein Receptor-related Protein
1997
Like other serpin-enzyme complexes (SECs), proteinase-complexed C1 inhibitor (C1-INH) is rapidly cleared from the circulation and thought to be a neutrophil chemoattractant, suggesting that complex formation causes structural rearrangements exposing a domain which is recognized by specific cell surface receptors. However, the cellular receptor(s) responsible for the catabolism and potential mediation of chemotaxis by C1-INH-protease complexes remained obscure. To determine whether the SEC receptor mediates the binding and potential chemotaxis of C1-INH·C1s, we performed binding assays with HepG2 cells, neutrophils, and monocytes, and the results show that C1-INH·C1s neither bind to these ce…
Novel evidences for a role of dopamine as modulator of intestinal motility: a study on mouse distal colon
2014
Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s disease. It has been classically considered that the pathological hallmarks of PD affect primarily the substantia nigra. Nevertheless, it has become increasingly evident that PD is a multicentric neurodegenerative process that affects several neuronal structures outside the substantia nigra, among which is the enteric nervous system (ENS). Pathological alterations within the ENS could be involved in the gastrointestinal (GI) dysfunction frequently encountered by PD patients. Dopamine (DA) seems to be a major candidate for the impairment of GI function in PD since its levels were found to be decrea…
Postnatal developmental changes in the dopaminergic signaling in mouse gut
2011
The presence of dopamine-containing neurons in the enteric nervous system has been described, but it is far to be clear their role in the modulation of gut motility. Moreover, ontogenetic studies shows that dopaminergic neurons are late-developing neurons, arising perinatally, but there are no informations if dopaminergic signaling may undergo to developmental changes after birth, as demonstrated for other signaling. Thus, using a pharmacological approach, we examined, in vitro, the role of dopaminergic neurons in the regulation of duodenal contractility in neonatal mice (≤48 h postnatal) compared to the adults. Transcripts for all dopaminergic receptors were detected in mouse duodenum at e…
Ecological study of the wood mouse helminth community in a burned Mediterranean ecosystem in regeneration five years after a wildfire
2007
AbstractParasites are used as biological tags in environmental impact studies. However, terrestrial systems in general and small mammals in particular are rarely considered in these ecological studies. Based on the effects of a wildfire which occurred in the Spanish Serra Calderona Natural Park — a typical Mediterranean ecosystem — the regeneration process of the wood mouse population and its helminth community is analysed. A total of 217 individuals of Apodemus sylvaticus were studied in a five year period, from the second to the fifth post-fire year: 152 mice originating from the burned area and 65 from the control — non-burned — area. The helminth community for both burned and non-burned…
A Reassessment of the Therapeutic Potential of a Dopamine Receptor 2 Agonist (D2-AG) in Endometriosis by Comparison against a Standardized Antiangiog…
2021
Dopamine receptor 2 agonists (D2-ags) have been shown to reduce the size of tumors by targeting aberrant angiogenesis in pathological tissue. Because of this, the use of a D2-ag was inferred for endometriosis treatment. When assayed in mouse models however, D2-ags have been shown to cause a shift of the immature vessels towards a more mature phenotype but not a significant reduction in the amount of vascularization and size of lesions. These has raised concerns on whether the antiangiogenic effects of these compounds confer a therapeutic value for endometriosis. In the belief that antiangiogenic effects of D2-ags in endometriosis were masked due to non-optimal timing of pharmacological inte…
Paracrine roles of extracellular vesicles released by mouse mesoangioblasts
2017
Extracellular vesicles (EV) represent an important mediator of cell-to-cell communication and are involved in both autocrine and paracrine signaling, with a critical role in a number of physiological and pathological conditions.1 The bioactive molecules contained within EV simultaneously activate several different pathways resulting in the synergistic stimulation of target cells. The discovery and characterization of EV have added a novel understanding to regenerative medicine, namely the finding that stem cells are an abundant source of EV.1-2 A6 mouse mesoangioblasts, vessel-associated multipotent progenitor stem cells that are capable of differentiating into different mesodermal cell typ…
Guanine-based purines affects the enteric cholinergic neurotransmission via a mechanism not involving membrane receptors
2011
Increasing evidence indicate that guanine-based purines, known as modulators of intracellular processes, can exert extracellular effects, raising the possibility of the existence of specific receptors for these compounds. We investigated if guaninebased purine receptors may be present in the rodent gastrointestinal tract modulating intestinal contractility, as the well known adenine-based purine receptors. Experiments were performed in vitro recording spontaneous and neurally-evoked contractile activity, as changes in isometric tension, in mouse distal colon circular muscle. Guanosine up to 3 mM or guanine up to 1 mM, did not affect the spontaneous mechanical activity, but they significantl…
Baseline Gut Microbiota Composition Is Associated With Schistosoma mansoni Infection Burden in Rodent Models
2020
In spite of growing evidence supporting the occurrence of complex interactions between Schistosoma and gut bacteria in mice and humans, no data is yet available on whether worm-mediated changes in microbiota composition are dependent on the baseline gut microbial profile of the vertebrate host. In addition, the impact of such changes on the susceptibility to, and pathophysiology of, schistosomiasis remains largely unexplored. In this study, mice colonized with gut microbial populations from a human donor (HMA mice), as well as microbiota-wild type (WT) animals, were infected with Schistosoma mansoni, and alterations of their gut microbial profiles at 50 days post-infection were compared to …
Interleukin 10 restores lipopolysaccharide-induced alterations in synaptic plasticity probed by repetitive magnetic stimulation
2020
Systemic inflammation is associated with alterations in complex brain functions such as learning and memory. However, diagnostic approaches to functionally assess and quantify inflammation-associated alterations in synaptic plasticity are not well-established. In previous work, we demonstrated that bacterial lipopolysaccharide (LPS)-induced systemic inflammation alters the ability of hippocampal neurons to express synaptic plasticity, i.e., the long-term potentiation (LTP) of excitatory neurotransmission. Here, we tested whether synaptic plasticity induced by repetitive magnetic stimulation (rMS), a non-invasive brain stimulation technique used in clinical practice, is affected by LPS-induc…
The NG2 Proteoglycan Protects Oligodendrocyte Precursor Cells against Oxidative Stress via Interaction with OMI/HtrA2.
2015
The NG2 proteoglycan is characteristically expressed by oligodendrocyte progenitor cells (OPC) and also by aggressive brain tumours highly resistant to chemo- and radiation therapy. Oligodendrocyte-lineage cells are particularly sensitive to stress resulting in cell death in white matter after hypoxic or ischemic insults of premature infants and destruction of OPC in some types of Multiple Sclerosis lesions. Here we show that the NG2 proteoglycan binds OMI/HtrA2, a mitochondrial serine protease which is released from damaged mitochondria into the cytosol in response to stress. In the cytosol, OMI/HtrA2 initiates apoptosis by proteolytic degradation of anti-apoptotic factors. OPC in which NG…