Search results for " myc"

Deciphering the molecular dialogue between arbuscular mycorrhizal partners during early root interactions

2009

International audience

La microdissection laser: une technique d’analyse du profil d’expression génique et protéique des types cellulaires spécifiques de la symbiose mycorh…

2012

Tracing of the inoculated arbuscular mycorrhizal fungal strain Glomus intraradices BEG140 in a field experiment over 3 years

2010

Is this fungus everywhere? Elucidating the genetic structure of populations of the arbuscular mycorrhizal fungus Glomus intraradices

2010

Arbuscular mycorrhizal fungi (Glomeromycota) are ubiquitous mutualistic associates of plants. Only relatively recently it has become possible to study the population biology of some species of these fungi. The model organism Glomus intraradices has been found across an extremely wide range of habitats and succession stages on different continents using molecular identification approaches. We used regions of the mitochondrially-encoded rDNA as novel molecular markers to resolve the intraspecies structure of G. intraradices and found a high diversity of mitochondrial haplotypes among isolates from all over the world. These markers for the first time also offer to genotype G. intraradices dire…

Arbuscular mycorrhiza : the biotrophic transportome from the cell to the field

2016

Understanding mechanisms underlying high nutrients use efficiency and carbon allocation in a context of mycorrhizal interactions is critical for sound management of croplands taking care of ecosystem services rendered by mycorrhizal fungi. Transport processes across the polarised membrane interfaces are of major importance in the functioning of the established mycorrhizal association as the symbiotic relation is based on a 'fair-trade' between fungus and host plant. Uptake and exchanges of nutrient and/or metabolites, at biotrophic interfaces are controlled by membrane transporters and their regulation patterns are essential in determining the outcome of plant fungal interactions and in ada…

BioVine project - Increase plant health through mycorrhizal fungi

2019

National audience; Organic vineyards still rely on large external inputs to control harmful organisms. BIOVINE will develop natural solutions based on plant diversity to control pests and reduce pesticide dependence. The capacity of plants of increasing the ecosystem resistance to pests and invasive species is a well-known ecosystem service. However, monocultures like vineyards do not exploit the potential of plant diversity. BIOVINE aims to develop new viticultural systems based on increased plant diversity within (e.g. cover crops) and/or around vineyards by planting selected plant species for the control of arthropods, soil-borne pests (oomycetes, fungi, nematodes), and foliar pathogens.…

Exploring the Genome of glomeromycotan fungi

2012

Chapitre 1; International audience; All fungi forming a mutualistic symbiosis with plant roots called arbuscular mycorrhiza were formerly grouped together in one order, the Glomales, placed in the Zygomycota (Morton 1993). Based on molecular analyses suggestingthat arbuscular mycorrhizal fungi should be separated from other fungal taxa, they were transferred a decade ago to the Glomeromy-cota , a new phylum created specifically for them (Schu¨ ssler et al. 2001). Whilst members of this monophyletic group originated from the same common ancestor as the Ascomycota and Basidiomycota, they have no obvious affinity to other major extant phylogenetic groups in the kingdom Fungi (James et al. 2006) …

Identification of sugar transporters in arbuscular mycorrhiza, from basic to applied science

2012

Our study focuses on sugar transporters from both plant and fungal partners at the symbiotic interface to better understand biotrophic exchange systems. Thereby, a collection of putative contigs and ESTs of hexose transporters from Medicago truncatula and Fragaria x ananassa will be processed. The full length sequences will be cloned for functional complementation and uptake experiments in transport deficient yeast mutants. This work also investigates the influence of different mycorrhizal fungi on (1) the expression level of sugar transporters and (2) the economically relevant part of F. x ananassa by analyzing its impact on the plant and the fruit. To this aim, combination of phenological…

Identification of sugar transporters in arbuscular mycorrhiza

2012

Our study focuses on sugar transporters from both plant and fungal partners at the symbiotic interface to better understand biotrophic exchange systems. Thereby, a collection of putative contigs and ESTs of hexose transporters from Medicago truncatula and Fragaria x ananassa will be processed. The full length sequences will be cloned for functional complementation and uptake experiments in transport deficient yeast mutants. This work also investigates the influence of different mycorrhizal fungi on (1) the expression level of sugar transporters and (2) the economically relevant part of F. x ananassa by analyzing its impact on the plant and the fruit. To this aim, combination of phenological…

Diversity of arbuscular mycorrhizal fungi (Glomeromycota) in european soils analysed by pyrosequencing

2012

Arbuscular mycorrhiza provides essential ecosystem functions in natural and humandominated ecosystems. Generally human activities like agriculture seem to have a negative effect on diversity of arbuscular mycorrhizal fungi (AMF), and thus on ecosystem functioning. New sequencing technologies now allow to assess AMF diversity on a much larger scale than previously. In the context of the European project EcoFINDERS, five Long-Term Observatories across Europe with different soil management have been studied. The diversity of AMF in these soils is analyzed by pyrosequencing, using the ITS (rDNA Internal Transcribed Spacers) as marker, which were recently determined as the standard barcoding gen…