Search results for " nuclei"

2012

Abstract. We present a numerical modelling study investigating the impact of mineral dust on cloud formation over the Eastern Mediterranean for two case studies: (i) 25 September 2008 and (ii) 28/29 January 2003. In both cases dust plumes crossed the Mediterranean and interacted with clouds forming along frontal systems. For our investigation we used the fully online coupled model WRF-chem. The results show that increased aerosol concentrations due to the presence of mineral dust can enhance the formation of ice crystals. This leads to slight shifts of the spatial and temporal precipitation patterns compared to scenarios where dust was not considered to act as ice nuclei. However, the total…

Frequent ultrafine particle formation and growth in Canadian Arctic marine and coastal environments

2017

The source strength and capability of aerosol particles in the Arctic to act as cloud condensation nuclei have important implications for understanding the indirect aerosol–cloud effect within the polar climate system. It has been shown in several Arctic regions that ultrafine particle (UFP) formation and growth is a key contributor to aerosol number concentrations during the summer. This study uses aerosol number size distribution measurements from shipboard expeditions aboard the research icebreaker CCGS Amundsen in the summers of 2014 and 2016 throughout the Canadian Arctic to gain a deeper understanding of the drivers of UFP formation and growth within this marine boundary layer. UFP nu…

Ship emissions measurement in the Arctic by plume intercepts of the Canadian Coast Guard icebreaker <i>Amundsen</i> from …

2016

Abstract. Decreasing sea ice and increasing marine navigability in northern latitudes have changed Arctic ship traffic patterns in recent years and are predicted to increase annual ship traffic in the Arctic in the future. Development of effective regulations to manage environmental impacts of shipping requires an understanding of ship emissions and atmospheric processing in the Arctic environment. As part of the summer 2014 NETCARE (Network on Climate and Aerosols) campaign, the plume dispersion and gas and particle emission factors of effluents originating from the Canadian Coast Guard icebreaker Amundsen operating near Resolute Bay, NU, Canada, were investigated. The Amundsen burned dist…

Phylogenetic analysis of Lymnaeid snails based on 18S rDNA sequences.

1997

The 18S rDNA sequences of the six most common European Lymnaeidae species (Mollusca:Gastropoda:Basommatophora) have been obtained by direct PCR cycle sequencing and silver staining methods. The sequence alignment and secondary structures of the 18S rRNA gene of Lymnaea stagnalis, L. auricularia, L. peregra, L. palustris, L. glabra, and L. truncatula are analyzed. This gene proves to be a good marker for both specific determination and supraspecific lymnaeid phylogeny. The malacological importance is evident, considering the specific determination problems of individual snails and the present systematic chaos in Lymnaeidae due to their pronounced morphoanatomic uniformity, which makes a clas…

Automatic cell nuclei counting: a protocol to acquire images and to compare results between color and multispectral images

2013

International audience; High-throughput screening in histology and analysis need a necessary automatic cell or nucleus counting. Current methods and systems based on grayscale or color images give results with counting errors. We suggest to use multispectral imaging (with more than three bands) rather than color one for nucleus counting. A traditional acquisition chains uses a source of white light and a CCD camera in addition to the optical microscope. To pass to a multispectral acquisition, we use a Programmable Light Source (PLS) in the place of the white light source. This PLS is capable of generating different wavelengths in the visible spectrum. So, one color image and four multispect…

Azaciclofanos modificados para el estudio de moléculas de interés biológico

2018

Como miembro investigador en grupo de Química Supramolecular de la Universitat de València el autor de la presente tesis ha orientado su trabajo a desarrollar y cumplir los objetivos marcados en el mismo, que consisten en el diseño, síntesis y estudio de compuestos bioactivos con acción antioxidante, antitumoral y/o antiparasitaria. Así pues, se diseñaron y sintetizaron siete nuevos ligandos poliamínicos para la obtención de nuevos complejos antioxidantes así como para evaluar su interacción con secuencias de ácidos nucleicos. Este proceso requirió, en algunos casos, del desarrollo y la puesta a punto de nuevas rutas sintéticas. Dado que las poliaminas poseen por definición varios grupos su…

Direct detection of repetitive, whole chromosome paint and telomere DNA probes by immunogold electron microscopy

1993

Biotinylated repetitive, whole chromosome paint and telomere DNA probes were investigated at the electron microscope level after non-isotopic in situ hybridization and direct immunogold detection. The protocol described allowed the visualization of a biotinylated chromosome 1 specific satellite DNA probe in the light microscope without silver intensification. This sensitive method was exploited to analyse factors contributing to signal strength in immunogold chromosome painting. Furthermore, it allowed us to investigate the distribution of (TTAGGG)n telomere repeats in human metaphase chromosomes and interphase nuclei. Telomeric and internal (TTAGGG)n repeats were detected at high spatial r…

A low repeat length in oligodendrocyte chromatin

1985

Abstract: The behavior of oligodendrocyte chromatin after micrococcal nuclease digestion of nuclei was assayed in brains of rats of four different ages. During oligodendrocyte differentiation, a decreasing sensitivity of the chromatin to enzymatic attack was observed. On the other hand, the nucleosomal repeat length showed a slight tendency to increase during development. It is worth noting that even the highest values reported here for “oligodendrocyte’ chromatin repeat lengths are significantly lower than 200 base pairs, the value previously reported by others for “non‐astrocytic glia.” Copyright © 1985, Wiley Blackwell. All rights reserved

Afferent and efferent projections of the dorsal anterior thalamic nuclei in the lizard Podarcis hispanica (Sauria, Lacertidae).

2002

The aim of this study was to investigate the afferent and efferent connections of the anterior thalamic nuclei in the lizard Podarcis hispanica. To identify potential sources of sensory inputs and to determine the fine organization of the projections of these thalamic nuclei to the telencephalon, we injected the sensitive tracer biotinylated dextran amine (BDA) into different nuclei of the anterior dorsal thalamus. We also injected BDA into several telencephalic areas in order to corroborate the results of thalamic injections. Our results show that the anterior thalamic nuclei receive projections from multiple areas and nuclei distributed throughout most of the brain, from rhombencephalon t…

Black hole lightning due to particle acceleration at subhorizon scales

2015

Supermassive black holes with masses of millions to billions of solar masses are commonly found in the centers of galaxies. Astronomers seek to image jet formation using radio interferometry, but still suffer from insufficient angular resolution. An alternative method to resolve small structures is to measure the time variability of their emission. Here, we report on gamma-ray observations of the radio galaxy IC 310 obtained with the MAGIC telescopes revealing variability with doubling time scales faster than 4.8 min. Causality constrains the size of the emission region to be smaller than 20\% of the gravitational radius of its central black hole. We suggest that the emission is associated …