Search results for " pigment"
showing 10 items of 309 documents
A Transition Zone Complex Regulates Mammalian Ciliogenesis and Ciliary Membrane Composition
2011
Mutations in genes encoding ciliary components cause ciliopathies, but how many of these mutations disrupt ciliary function is unclear. We investigated Tectonic1 (Tctn1), a regulator of mouse Hedgehog signaling, and found that it is essential for ciliogenesis in some, but not all, tissues. Cell types that do not require Tctn1 for ciliogenesis require it to localize select membrane-associated proteins to the cilium, including Arl13b, AC3, Smoothened and Pkd2. Tctn1 forms a complex with multiple ciliopathy proteins associated with Meckel (MKS) and Joubert (JBTS) syndromes, including Mks1, Tmem216, Tmem67, Cep290, B9d1, Tctn2, and Cc2d2a. Components of the Tectonic ciliopathy complex colocaliz…
Taurine in the interphotoreceptor matrix
2014
TAURINE IN THE INTERPHOTORECEPTOR MATRIX Gueli Maria Concetta Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche (BioNEC), Università degli Studi di Palermo Taurine (Tau) is the most abundant amino compound free in the retina. It is concentrated in the photoreceptor inner segment, in the outer nuclear layer and in the synapses. The retina synthesizes and receives Tau from choroidal blood via the pigment epithelium (PE). The high content in the retina suggest the possibily of verifying whether it was present in the interphotoreceptor matrix (IPM), which occupies the subretinal space. In this study we have determined the Tau level in the IPM, separating it from other soluble ami…
Multi-Technique Diagnostic Investigation in View of the Restoration of “The Glory of St. Barbara” Painting by Mattia Preti
2022
The present paper illustrates the results of a diagnostic investigation performed on the oil on canvas painting “The Glory of St. Barbara” (1680–1688) by Mattia Preti. The painting is located inside the St. Barbara Church in Taverna (Catanzaro, Italy), the city that gave birth to the artist. In situ, non-invasive studies, by applying X-ray fluorescence (XRF) spectroscopy, along with laboratory micro-destructive analytical investigations, by employing electron probe microanalyses (EPMA) coupled with energy-dispersive spectrometry (EDS) and micro-Raman (µ-Raman) spectroscopy, were combined to retrieve the color palette and identify the painting technique and style of t…
Differences in the activity and distribution of peroxidase from three different portions of germinative Brassica oleracea seeds.
2002
Peroxidase (POD, EC 1.11.1.7) activity, cellular localization and isozyme patterns were investigated in the seed integument, cotyledon and embryo axis of Brassica oleracea cv. Cappuccio during pregermination and seedling growth. Seeds started to germinate after 24 h of imbibition. POD activity was localized in the pigmented layer of the integument and in procambial strands of the cotyledon and embryo axis in the first 24 h of imbibition. It was localized in the integumental cells of palisade, pigmented and aleurone layers and in epidermal, meristematic, procambial cells and xylem elements of the root and hypocotyl after 48 h of imbibition. POD activity increased during germination and early…
Aproximacion funcional a CERKL, un gen causante de la retinosis pigmentaria, mediante el estudio de la localización intracelular de la proteína y la …
2015
La proteína CERKL se encuentra localizada tanto en el núcleo como en el citoplasma. En el citoplasma, CERKL se distribuye de manera difusa y también formando unos agregados que en su mayor parte se distribuyen en la periferia nuclear. Esos agregados colocalizan con marcadores de gránulos de estrés y su cantidad aumenta sometiendo las células a estrés (arsenito de sodio o choque térmico) y disminuye tras un tratamiento con cicloheximida (un inhibidor de la síntesis de proteínas que desensambla los gránulos de estrés). La entrada y salida de CERKL del núcleo es necesaria para su localización en los gránulos de estrés y, por eso, el mutante patológico C125W que está ausente de los núcleos no c…
Study of USH1 Splicing Variants through Minigenes and Transcript Analysis from Nasal Epithelial Cells
2012
Usher syndrome type I (USH1) is an autosomal recessive disorder characterized by congenital profound deafness, vestibular areflexia and prepubertal retinitis pigmentosa. The first purpose of this study was to determine the pathologic nature of eighteen USH1 putative splicing variants found in our series and their effect in the splicing process by minigene assays. These variants were selected according to bioinformatic analysis. The second aim was to analyze the USH1 transcripts, obtained from nasal epithelial cells samples of our patients, in order to corroborate the observed effect of mutations by minigenes in patient’s tissues. The last objective was to evaluate the nasal ciliary beat fre…
Indagine composizionale sulle pitture di due vasi di Centuripe
2019
Due vasi Centuripe a Palermo sono stati analizzati utilizzando XRF portatile complementare e FTIR a riflessione totale spettroscopie. Le analisi consentono di chiarire la tavolozza e la tecnica della decorazione (pittura a tempera con un legante organico), e mostrano la presenza di pigmenti sia antichi che moderni (a causa di qualche recente riverniciatura). Uno dei risultati più interessanti è stata l'identificazione di due fasi del solfato di calcio nello strato sottostante i dipinti: a causa delle condizioni che trasformano il gesso in bassanite, la presenza di il gesso o, in alternativa, la bassanite in ciascuna parte analizzata possono rappresentare un criterio per distinguere l'origin…
Tekstīliju krāsošanas procesā izmantoto pigmentu pētījumi, pielietojot hromatogrāfiskās analīzes metodes
2018
Tekstīliju krāsošanas procesā izmantoto pigmentu pētījumi, pielietojot hromatogrāfiskās analīzes metodes. Bobrovs A., zinātniskā vadītāja docente, Dr.Ķīm. Ilva Nakurte. Bakalaura darbs, 74 lappuses, 79 attēli, 32 literatūras avoti, 6 pielikumi. Latviešu valodā. Literatūras apskatā apkopota informācija par krāsvielām un pigmentiem, to izcelsmi, ķīmisko satāvu un pētīšanas metodēm. Aprakstīti dabīgie audumu krāsošanas veidi Latvijā. Darbā praktiski pētīti un raksturoti divi dabiski ar Oreganum Vulgare un Malus Domestica krāsoti dzijas paraugi, kā arī trīs dziju paraugi no vēsturiskām tekstīlijām, izmantojot šķidrumu hromatogrāfiju tandēmā ar diožu matricas detektoru un augstas izšķirtspējas m…
Mosaic activating mutations in GNA11 and GNAQ are associated with phakomatosis pigmentovascularis and extensive dermal melanocytosis
2016
Common birthmarks can be an indicator of underlying genetic disease but are often overlooked. Mongolian blue spots (dermal melanocytosis) are usually localized and transient, but they can be extensive, permanent, and associated with extracutaneous abnormalities. Co-occurrence with vascular birthmarks defines a subtype of phakomatosis pigmentovascularis, a group of syndromes associated with neurovascular, ophthalmological, overgrowth, and malignant complications. Here, we discover that extensive dermal melanocytosis and phakomatosis pigmentovascularis are associated with activating mutations in GNA11 and GNAQ, genes that encode Gα subunits of heterotrimeric G proteins. The mutations were det…