Search results for " polyaspartamide"

showing 10 items of 14 documents

FLUORINATED DERIVATIVES OF A POLYASPARTAMIDE BEARING POLYETHYLENE GLYCOL CHAINS AS OXYGEN CARRIERS

2008

Abstract In this paper the synthesis and characterization of new fluorinated polymers based on a polyaspartamide bearing polyethylene glycol (PEG) chains, are reported. The starting material was the α,β-poly(N-2-hydroxyethyl)- dl -aspartamide (PHEA), a water soluble and biocompatible polymer, that has been derivatized with both polyethylene glycol (with a molecular weight of 2000 Da) and 5-pentafluorophenyl-3-perfluoroheptyl-1,2,4-oxadiazole. By varying the amount of the fluorinated oxadiazole, three samples have been prepared and characterized by FT-IR, 1H NMR, 19F NMR and UV–VIS spectroscopy. Size exclusion chromatography analysis of these copolymers revealed the occurrence of a self-asso…

Aqueous solutionBiocompatibilityChemistryOrganic ChemistrySize-exclusion chromatographyOxadiazoleFluorine-19 NMRPolyethylene glycolHaemolysisBiochemistryInorganic Chemistrychemistry.chemical_compoundDynamic light scatteringARTIFICIAL OXYGEN CARRIER POLYASPARTAMIDE FLUORINATED POLYMERIC MICELLESSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoPolymer chemistryEnvironmental ChemistryPhysical and Theoretical Chemistry
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Polyhydroxyethylaspartamide-spermine copolymers: Efficient vectors for gene delivery

2008

Abstract Aim of this paper was that to prepare biocompatible, polyaspartamide based copolymers containing spermine or spermine/hydrophobic side chains able to condense nucleic acids and to transfect mammalian cells. Copolymers were prepared starting from α,β-poly-(N-2-hydroxyethyl)- d , l -aspartamide (PHEA) and exploiting the reactive hydroxyl groups in the polymeric side chains by subsequent activation reactions to obtain PHEA-Spermine (PHEA-Spm) and PHEA-Spermine-Butyramide (PHEA-Spm-C4). Molecular, physico-chemical and biological characterization of copolymers and interpolyelectrolyte complexes with plasmid DNA was performed. Experimental results evidenced that these copolymers are able…

Biocompatibilitygene delivery polyaspartamideCell SurvivalStereochemistryPharmaceutical ScienceSpermineGene deliveryBiologyTransfectionpolycationDNA Adductschemistry.chemical_compoundCell Line TumorCopolymerHumansLuciferasesCells CulturedErythrocyte MembraneGenetic transferinterpolyelectrolyte complexesGene Transfer TechniquesDNATransfectionCombinatorial chemistrychemistryNucleic acidSperminePeptidesDNAJournal of Controlled Release
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Cationic polyaspartamide-based nanocomplexes mediate siRNA entry and down-regulation of the pro-inflammatory mediator high mobility group box 1 in ai…

2015

Abstract High-mobility group box 1 (HMGB1) is a nonhistone protein secreted by airway epithelial cells in hyperinflammatory diseases such as asthma. In order to down-regulate HMGB1 expression in airway epithelial cells, siRNA directed against HMGB1 was delivered through nanocomplexes based on a cationic copolymer of poly(N-2-hydroxyethyl)- d,l -aspartamide (PHEA) by using H441 cells. Two copolymers were used in these experiments bearing respectively spermine side chains (PHEA-Spm) and both spermine and PEG2000 chains (PHEA-PEG-Spm). PHEA-Spm and PHEA-PEG-Spm derivatives complexed dsDNA oligonucleotides with a w/w ratio of 1 and higher as shown by a gel retardation assay. PHEA-Spm and PHEA-P…

Polyaspartamide copolymerNucleic acid-based drugDown-RegulationPharmaceutical ScienceSpermineRespiratory MucosaBiologyTransfectionAirway epithelial cellsNucleic acid-based drugsFlow cytometrychemistry.chemical_compoundCell Line TumorMaterials TestingAirway epithelial cellmedicineHumansElectrophoretic mobility shift assayMTT assayDAPIRNA Small InterferingCytotoxicityPolyhydroxyethyl MethacrylateHMGB1Airway epithelial cells; HMGB1; Nucleic acid-based drugs; PHEA; Polyaspartamide copolymers; Sirnamedicine.diagnostic_testOligonucleotideMammaglobin AfungiGene Transfer TechniquesEpithelial CellsDNAPHEAMolecular biologyNanostructuresPolyaspartamide copolymerschemistrySirnaTrypan bluePeptides
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TOWARD POTENT ANTIBIOFILM DEGRADABLE MEDICAL DEVICES: GENERIC METHODOLOGIES FOR THE SURFACE MODIfiCATION OF POLYLACTIDE

2014

PolyaspartamidePLA surfaceantibiofilmPLA surfacesantibiofilm PolyaspartamideThiol-Yneantibiofilm; Polyaspartamide; PLA surfaces; Thiol-Yne
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Polyaspartamide based microparticles for Tobramycin delivery to the lung in FC therapy

2015

PolyaspartamidemicroparticlesTobramycin Polyaspartamide microparticles FC therapyFC therapyTobramycin; Polyaspartamide; microparticles; FC therapyTobramycin
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Biodegradable and pH-sensitive hydrogels for potential colon-specific drug delivery: Characterization and in vitro release studies

2008

A novel pH-sensitive and biodegradable composite hydrogel, based on a methacrylated and succinic derivative of dextran, named Dex-MA-SA, and a methacrylated and succinic derivative of alpha,beta-poly( N-2-hydroxyethyl)- DL-aspartamide (PHEA), named PHM-SA, was produced by photocross-linking. The goal was to obtain a colon-specific drug delivery system, exploiting both the pH-sensitive behavior and the colon-specific degradability. The hydrogel prepared with a suitable ratio between the polysaccharide and the polyaminoacid was characterized regarding its swelling behavior in gastrointestinal simulated conditions, chemical and enzymatic degradability, interaction with mucin, and cell compatib…

Polymers and PlasticsBiocompatibilityCell SurvivalColonBioengineeringBiomaterialschemistry.chemical_compoundDrug Delivery SystemsSpectroscopy Fourier Transform InfraredMaterials ChemistrymedicineMucoadhesionHumansDextranaseChromatographydextran polyaspartamide colon releaseChemistryMucinsHydrogelsHydrogen-Ion ConcentrationDextranBiochemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDrug deliverySelf-healing hydrogelsCaco-2 CellsSwellingmedicine.symptomDrug carrier
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MODIFIED PEPTIDE RELEASE FROM NEW IN SITU GEL FORMING COPOLYMERS BASED ON POLYLACTIDE AND POLYASPARTAMIDE

2008

Settore CHIM/09 - Farmaceutico Tecnologico ApplicativoPEPTIDE RELEASE GEL POLYASPARTAMIDE POLYESTER
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PEGYLATED PHOSPHOLIPID-POLYASPARTAMIDE COPOLYMERS AS SELF-ASSEMBLING COLLOIDAL DRUG DELIVERY SYSTEMS

2009

Settore CHIM/09 - Farmaceutico Tecnologico Applicativophospholipids polyaspartamide drug delivery systems
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Synthesis and characterization of polyaspartamide copolymers obtained by ATRP for nucleic acid delivery

2014

Abstract Nucleic acid molecules such as small interfering RNAs (siRNAs) and plasmidic DNAs (pDNAs) have been shown to have the potential to be of therapeutic value in different human diseases. Their practical use is however compromised by the lack of appropriate release systems. Delivered as naked molecules, siRNAs/pDNAs are rapidly degraded by extracellular nucleases thus considerably reducing the amount of molecule which can reach the target cells. Additionally, the anionic charge of the phosphate groups present on the siRNAs/pDNAs backbone, disfavors the interaction with the negatively charged surface of the cell membrane. In this paper we describe the generation of a novel polymer able …

Small interfering RNACell SurvivalPharmaceutical ScienceATRPMethacrylateTransfectionsiRNA; deliveryPolymerizationchemistry.chemical_compoundMiceSiRNA delivery; DNA delivery; Polyaspartamide; ATRPCell Line TumorPolymer chemistryCopolymerAnimalsHumansRNA MessengerRNA Small Interferingchemistry.chemical_classificationAtom-transfer radical-polymerizationPolymerDNACombinatorial chemistryPolyaspartamideMonomerchemistryPolymerizationsiRNANucleic acidSiRNA deliveryMethacrylatesdeliveryPeptidesE2F1 Transcription FactorDNA deliveryPlasmids
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Polymeric micelles based on a polyaspartamide copolymer for pulmonary delivery of beclomethasone dipropionate

2015

beclomethasone dipropionatepolyaspartamidepulmonary deliveryPolymeric micellesPolymeric micelles; polyaspartamide; pulmonary delivery; beclomethasone dipropionatePolymeric micelles polyaspartamide pulmonary delivery beclomethasone dipropionate
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