Search results for " precipitation"

Microradiography and confocal laser scanning microscopy applied to enamel lesions formed in vivo with and without fluoride varnish treatment

1996

The aim of the present investigation was to combine 2 techniques suitable for lesion characterization: quantitative microradiography (TMR) and confocal laser scanning microscopy (CLSM) on in vivo induced lesions with and without a fluoride varnish (Duraphat(R)) treatment. Orthodontic bands were attached to premolars to be extracted for orthodontic reasons to induce enamel caries on the buccal surfaces. In the caries development part of the study, 1 tooth of each pair received at the start 1 topical application with the fluoride varnish Duraphat(R), the contralateral tooth serving as untreated control. All teeth were extracted after 4 weeks. In the caries progression part, the premolars were…

Magnetic Properties of Ni II Cr III Layered Double Hydroxide Materials

2008

This paper describes the isolation of four layered double hydroxide (LDH) compounds having the general formula[NiII3–xCrIIIx(OH)6](CO3)x/2·yH2O [x = 0.57 (1), 0.69 (2), 0.81 (3) and 0.93 (4)] by using homogeneous precipitation methods and varying the metal ratio in the synthetic solutions. All the reported compounds have carbonate anions in the interlamellar space. This fact forces the interlayer distances to remain unchanged in all the cases, thus providing an ideal system in which the changes observed in magnetic properties can be correlated with metal composition along the hydroxide layers.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)

Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae

2021

The quality and consistency in every sample preparation procedure is crucial for any scientific output. Therefore, it is of utmost importance to have easy, economic, and robust sample preparation protocols. Here, we describe a simple and robust bottom-up proteomic sample preparation strategy for identification and label-free quantification (LFQ) of proteins and phosphoproteins. The presented workflow is designed for large-scale application and involves easy scalable and well-known robust sample preparation techniques, such as cell lysis with SDS buffer under heat, protein precipitation using methanol/chloroform, tryptic digest, and commercially available TiO2 phosphopeptide enrichment kits.…

Data Resolution Effects on Landslides Hazard and Susceptibility Assessment of Puerto Rico

2008

The removal of sulphate from mine water by precipitation as ettringite and the utilisation of the precipitate as a sorbent for arsenate removal.

2016

Abstract The aim of this research was to investigate sulphate removal from mine water by precipitation as ettringite (Ca6Al2(SO4)3(OH)12·26H2O) and the utilisation of the precipitate as a sorbent for arsenate removal. The mine water sulphate concentration was reduced by 85–90% from the initial 1400 mg/L during ettringite precipitation depending on the treatment method. The precipitation conditions were also simulated with MINEQL + software, and the computational results were compared with the experimental results. The precipitated solids were characterised with X-ray diffraction and a scanning electron microscope. The precipitated solids were tested as sorbents for arsenate removal from the…

Heavy metal sorption in the lichen cationactive layer.

2007

Results of copper ion sorption in lichens owing to the ion exchange between the surroundings (aqueous solution) and the lichen cationactive layer have been presented. It indicates that the course of sorption of these ions, similarly as in the case of cations of other heavy metals, depends on the concentration and type of cations naturally found in lichen surroundings: H+, Na+, K+, Mg2+ and Ca2+. A determination method of heavy metal concentration in lichen surroundings has been proposed. It consists in exposure of transplanted lichens in the presence of salts that provide precisely determined, artificial salinity of precipitation with which the lichens are in contact. The studies were condu…

Determination of globotriaosylceramide in plasma and urine by mass spectrometry

2009

Abstract Background: Fabry disease is an X-chromosomally inherited lysosomal storage disorder leading to accumulation of glycosphingolipids, mainly globotriaosylceramide (ceramide-trihexoside, Gb3). Concentrations of Gb3 in plasma and urine have been used to diagnose Fabry disease and to monitor enzyme replacement therapy with recombinant α-galactosidase. Methods: Gb3 was purified from plasma or urine by combined liquid extraction/protein precipitation and solid-phase extraction, and was detected by flow-injection analysis electrospray mass spectrometry (MS) using multi-reaction-monitoring. Calibration was performed via standard addition using C17-Gb3 as internal standard. The most abundant…

The invariant chains of mouse class II antigens: biochemical properties and molecular relationship

1984

The proteins p40 (Mr = 40 000), p32 (Mr = 32 000), p28 (Mr = 28 000), p20 (Mr = 20 000) and p10 (Mr = 10 000) are described which occur in noncovalent association with the polymorphic alpha, beta heterodimer of class II antigens. They were investigated with respect to their molecular characteristics and their mutual structural relationship. p32, the predominant species of this group corresponds to the invariant chain gamma (Ii). In contrast to the polymorphic subunits alpha and beta, proteins p40, p28, p20 and p10 migrated like gamma in electrophoretically constant positions, when class II molecules of different subregions and different alleles were assessed by two-dimensional gel electroph…

Determination of benzophenone-3 and its main metabolites in human serum by dispersive liquid–liquid microextraction followed by liquid chromatography…

2013

A new analytical method for the determination of benzophenone-3 (2-hydroxy-4-methoxybenzophenone), and its main metabolites (2,4-dihydroxybenzophenone and 2,2'-dihydroxy-4-methoxybenzophenone) in human serum is presented. The method is based on dispersive liquid-liquid microextraction (DLLME) as preconcentration and clean-up technique, followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Acidic hydrolysis and protein precipitation with HCl 6 M (1:1) (100 °C, 1 h) were carried out before extraction. The variables involved in the DLLME process were studied. Under the optimized conditions, 70 µL of acetone (disperser solvent) and 30 µL of chloroform (extraction solvent) were …

IL-6 receptor independent stimulation of human gp130 by viral IL-6.

2000

Abstract The genome of human herpes virus 8, which is associated with Kaposi’s sarcoma, encodes proteins with similarities to cytokines and chemokines including a homologue of IL-6. Although the function of these viral proteins is unclear, they might have the potential to modulate the immune system. For viral IL-6 (vIL-6), it has been demonstrated that it stimulates IL-6-dependent cells, indicating that the IL-6R system is used. IL-6 binds to IL-6R, and the IL-6/IL-6R complex associates with gp130 which dimerizes and initiates intracellular signaling. Cells that only express gp130 but no IL-6R cannot be stimulated by IL-6 unless a soluble form of the IL-6R is present. This type of signaling…