Search results for " processing"
showing 10 items of 7549 documents
Protein tyrosine nitration and thiol oxidation by peroxynitrite-strategies to prevent these oxidative modifications.
2013
The reaction product of nitric oxide and superoxide, peroxynitrite, is a potent biological oxidant. The most important oxidative protein modifications described for peroxynitrite are cysteine-thiol oxidation and tyrosine nitration. We have previously demonstrated that intrinsic heme-thiolate (P450)-dependent enzymatic catalysis increases the nitration of tyrosine 430 in prostacyclin synthase and results in loss of activity which contributes to endothelial dysfunction. We here report the sensitive peroxynitrite-dependent nitration of an over-expressed and partially purified human prostacyclin synthase (3.3 μM) with an EC50 value of 5 μM. Microsomal thiols in these preparations effectively co…
Single-cell analysis of population context advances RNAi screening at multiple levels
2012
Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…
Method and Practical Guidelines for Overcoming Enterprise Architecture Adoption Challenges
2017
During the last few years, interest towards Enterprise Architecture (EA) has increased, not least due to anticipated benefits resulting from adopting it. For instance, EA has been argued to provide cost reduction, technology standardisation, process improvement, and strategic differentiation. Despite these benefits, the EA adoption rate and maturity are still low. Consequently, EA benefits are not realised. A major reason hindering the adoption is that EA is not understood correctly. This paper aims for minimising the effect of the lack of understanding EA to adopting EA. Based on the research conducted in Finnish public sector, we propose an improved Enterprise Architecture Adoption Method…
Measuring syntactic complexity in learner Finnish
2020
In the study of complexity, accuracy and fluency (CAF), syntactic complexity can be measured by a multitude of measures. Traditionally, the measures are quantitative and they use production units such as words, clauses, T -units, and sentences. Despite the vast number o f measures available, many studies have used only one or two of them, or parallel ones tapping the same component of complexity. The present study explores syntactic complexity using seven frequently used quantitative complexity measures to gauge different facets of complexity in written learner Finnish. The data of the study consist of texts written by adult and adolescent language learners, and they cover proficiency level…
Words, clauses, sentences, and T-units in learner language: Precise and objective units of measure?
2020
In research on learner language complexity, accuracy and fluency (CAF), syntactic complexity is often studied with quantitative measures based on words, clauses, sentences, and T-units. The findings have been mixed, but segmenting learner language into these units of measure has seldom been problematised, even if the need for accurate coding is well known. The present study explores words, clauses, sentences, and T-units as production units in written learner language using a corpus of 352 L2 Finnish texts (28,813 words). The results illustrate how written learner language can be hard to fit into the production unit categories, which are essential for the most frequently used quantitative m…
New is not always costly: evidence from online processing of topic and contrast in Japanese.
2013
Two visual ERP experiments were conducted to investigate topic and contrast assigned by various cues such as discourse context, sentential position and marker during referential processing in Japanese. Experiment 1 showed that there was no N400-difference for new vs. given noun phrases (NPs) when the new NP was expected (contrastively focused) based on its preceding context and sentential position. Experiment 2 further revealed that the N400 for new NPs can be modulated by the NP’s contrastive meaning (exhausitivity) induced from the marker. Both experiments also showed that new NPs engendered an increased Late Positivity. The reduced N400 for new vs. given supports an expectation-based lin…
Some Remarks about Product Spaces
2018
Summary This article covers some technical aspects about the product topology which are usually not given much of a thought in mathematics and standard literature like [7] and [6], not even by Bourbaki in [4]. Let {Ti}i∈I be a family of topological spaces. The prebasis of the product space T = ∏ i∈I Ti is defined in [5] as the set of all π −1 i (V) with i ∈ I and V open in Ti . Here it is shown that the basis generated by this prebasis consists exactly of the sets ∏ i∈I Vi with Vi open in Ti and for all but finitely many i ∈ I holds Vi = Ti . Given I = {a} we have T ≅ Ta , given I = {a, b} with a≠ b we have T ≅ Ta ×Tb . Given another family of topological spaces {Si}i∈I such that Si ≅ Ti fo…
Anonymization as homeomorphic data space transformation for privacy-preserving deep learning
2021
Industry 4.0 is largely data-driven nowadays. Owners of the data, on the one hand, want to get added value from the data by using remote artificial intelligence tools as services, on the other hand, they concern on privacy of their data within external premises. Ideal solution for this challenge would be such anonymization of the data, which makes the data safe in remote servers and, at the same time, leaves the opportunity for the machine learning algorithms to capture useful patterns from the data. In this paper, we take the problem of supervised machine learning with deep feedforward neural nets and provide an anonymization algorithm (based on the homeomorphic data space transformation),…
Skeleton-Based Multiview Reconstruction
2016
International audience; The advantage of skeleton-based 3D reconstruction is to completely generate a single 3D object from well chosen views. Having numerous views is necessary for a reliable reconstruction but projections of skeletons lead to different topologies. We reconstruct 3D objects with curved medial axis (whose topology is a tree) from the perspective skeletons on an arbitrary number of calibrated acquisitions. The main contribution is to estimate the 3D skeleton, from multiple images: its topology is chosen as the closest to those of the perspective skeletons on the set of images, which means that the number of topology changes to map the 3D skeleton topology to topologies on im…
Ergonomie des documents techniques informatisés : expériences et recommandations sur l'utilisation des organisateurs para-linguistiques
1998
Despite an increasing development, the design of computerized documents is still based on intuitive or esthetics concerns, rather than on experimental evidence. However a large body of literature has investigated the effects of various display factors, both on a perceptual and on a cognitive level. We propose here a review of research and its implication on the design of computerized documents.