Search results for " purification"

showing 10 items of 244 documents

Regeneration of total RNA purification silica-based columns

2010

Silica-based columns are largely used in RNA purification, allowing fast extractions and good yields of high quality nucleic acid, but their major limitation is the high cost. The reuse of such columns, although desirable, is not recommended because of residual amounts of material from the previous sample trapped in the column matrix, which might be released during further purification. Thus, recycling does need previous complete removal of any detectable RNA trace, but to date no protocol which allows decontamination and reuse is available.We report a very rapid decontamination procedure, based on treatment with warm alkaline solution containing Triton X-100, which ensures no RNA carry-ove…

OctoxynolClinical BiochemistryTotal rnatotal RNA purificationSettore BIO/11 - Biologia MolecolareReuseAlkaliesBiochemistryAnalytical ChemistrySettore BIO/10 - BiochimicaDrug DiscoveryRecyclingalkaline treatmentMolecular BiologyDecontaminationPharmacologyColumn vectorChromatographyChromatographyChemistryRegeneration (biology)RNAGeneral MedicineHuman decontaminationsilica-based spin-columSilicon DioxideregenerationNucleic acidRNARNA extractionTriton-X 100 treatment
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Biomass-based composite catalysts for catalytic wet peroxide oxidation of bisphenol A:preparation and characterization studies

2019

The wet granulation process was used to prepare new, efficient, and cost-effective granular biomass-based composite catalysts for catalytic wet peroxide oxidation (CWPO) of bisphenol A (BPA). The most stable composite granules was prepared by mixing biomass-based carbon residue (CR) with metakaolin (MK) combined with calcium oxide (CaO) or cement and a solvent (NaOH or KOH). For all the prepared composite granules, the optimized binding agents to carbon ratio was 0.3, the solvent to carbon ratio 1.2, and the agitation rate 1200 rpm. The specific surface area of the prepared catalysts was 152–205 m2/g. The composite granular catalyst (CR + MK + CaO + NaOH) had the most durable and stable str…

Oxidation of bisphenol AGranular activated carbonaktiivihiiliComposite catalystWastewater purificationbiomassa (teollisuus)jätevesiBiomasskomposiititjäteveden käsittely
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A multivariate approach of changes in filamentous, nitrifying and protist communities and nitrogen removal efficiencies during ozone dosage in a full…

2019

[EN] The application of low ozone dosage to minimize the problems caused by filamentous foaming was evaluated in two bioreactors of an urban wastewater treatment plant. Filamentous and nitrifying bacteria, as well as protist and metazoa, were monitored throughout a one-year period by FISH and conventional microscopy to examine the effects of ozone application on these specific groups of microorganisms. Multivariate data analysis was used to determine if the ozone dosage was a key factor determining the low carbon and nitrogen removal efficiencies observed throughout the study period, as well as to evaluate its impact on the biological communities monitored. The results of this study suggest…

Ozone010504 meteorology & atmospheric sciencesNitrogenHealth Toxicology and MutagenesisMicroorganismSegmented filamentous bacteriaWastewater010501 environmental sciencesToxicology01 natural sciencesWater Purificationchemistry.chemical_compoundOzoneAmmoniaOzonationBioreactorTECNOLOGIA DEL MEDIO AMBIENTE0105 earth and related environmental sciencesSewagebiologyMicrobiotaGeneral Medicinebiology.organism_classificationNitrificationPollutionFilamentous bacteriaActivated sludgeMultivariate analysischemistryActivated sludgeNitrifying bacteriaEnvironmental chemistryDenitrificationSewage treatmentNitrificationEnvironmental Pollution
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Recent Exploits of the ISOLTRAP Mass Spectrometer

2013

Abstract The Penning-trap mass spectrometer ISOLTRAP, located at the isotope-separator facility ISOLDE (CERN), is presented in its current form taking into account technical developments since 2007. Three areas of developments are presented. The reference ion sources have been modified to guarantee a sufficient supply of reference ions for mass measurements and systematic studies. Different excitation schemes have been investigated for manipulation of the ion motion in the Penning trap, to enhance either the purification or measurement process. A multi-reflection time-of-flight mass separator has been implemented and can now be routinely used for purification and as a versatile tool for bea…

Penning-trap mass spectrometryNuclear and High Energy PhysicsLarge Hadron ColliderIon beam analysisChemistry010401 analytical chemistryMeasurement of pure ion ensembles[PHYS.NEXP]Physics [physics]/Nuclear Experiment [nucl-ex]Mass spectrometryPenning trap01 natural sciencesISOLTRAPMulti-reflection time-of-flight mass separator0104 chemical sciencesSecondary ion mass spectrometryNuclear physicsIon-beam analysis0103 physical sciencesBeam purificationIon trapAtomic physics010306 general physicsNuclear ExperimentInstrumentationHybrid mass spectrometer
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Optimization of coupled advanced oxidation processes and activated carbons for purification of salt water

2015

Photocatalysis, ozonation and activated carbons were investigated separately and in combination as tools for the purification of polluted salt water. Coupling different processes enables to overcome many drawbacks related to the use of the single technologies and at the same time to exploit possible synergistic effects. In this work a kinetic analysis was performed for modelling the degradation of 4-nitrophenol (4NP) as a probe pollutant molecule in synthetic seawater. Thus, the optimum synergistic conditions of the three processes acting together were determined and discussed.

Photocatalysis activated carbons salt water purification nitrophenol
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PIPERADE: A double Penning trap for mass separation and mass spectrometry at DESIR/SPIRAL2

2021

International audience; A double Penning trap is being commissioned at CENBG Bordeaux for the future DESIR/SPIRAL2 facility of GANIL. The setup is designed to perform both high-resolution mass separation of the ion beam for trap-assisted spectroscopy, and high-accuracy mass spectrometry of short-lived nuclides. In this paper, the technical details of the new device are described. First offline tests with the purification trap are also presented, showing a mass resolving power of about 105.

PhysicsNuclear and High Energy PhysicsSpeichertechnik - Abteilung BlaumMass spectrometryIon beamPenning trap010401 analytical chemistryMass spectrometryPenning trap01 natural sciencesMass separation0104 chemical sciencesNuclear physicsTrap (computing)0103 physical sciencesBeam purificationNew device[PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det]Nuclide010306 general physicsSpectroscopyInstrumentationDESIR/SPIRAL2
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Semiconductor mixed oxides as innovative materials for the photocatalytic removal of organic pollutants

2020

Abstract This work aims to provide a critical survey of the extensive literature concerning the potential applications of nanostructured mixed oxides for environmental remediation. In particular, our attention was focused on the feasibility to employ these materials as photocatalysts for the elimination of toxic organic pollutants contained in water and wastewater. The advantages and perspectives of the various nanocomposites are briefly discussed.

PollutantWaste managementWater purificationbusiness.industryEnvironmental remediationEnvironmental remediationOrganic pollutant removalMixed oxidesNanostructuresHeterogeneous photocatalysisSemiconductorWork (electrical)WastewaterPhotocatalysisEnvironmental scienceCritical surveySettore CHIM/07 - Fondamenti Chimici Delle Tecnologiebusiness
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Environmental and economic profile of six typologies of wastewater treatment plants

2011

The objective of wastewater treatment plants (WWTPs) is to prevent pollution. However, it is necessary to assess their sustainability in order to ensure that pollution is being removed, not displaced. In this research, the performance of 24 WWTPs has been evaluated using a streamlined Life Cycle Assessment (LCA) with Eutrophication Potential (EP) and Global Warming Potential (GWP) as environmental indicators, and operational costs as economic indicators. WWTPs were further classified in six typologies by their quality requirements according to their final discharge point or water reuse. Moreover, two different functional units (FU), one based on volume (m(3)) and the other on eutrophication…

PollutionEnvironmental EngineeringEcological Modelingmedia_common.quotation_subjectEnvironmental engineeringEnvironmentEutrophicationReuseGlobal WarmingWaste Disposal FluidPollutionWater PurificationEconomic indicatorSpainSustainabilityEnvironmental scienceSewage treatmentEutrophicationWaste Management and DisposalLife-cycle assessmentWater Science and TechnologyCivil and Structural Engineeringmedia_commonWaste disposalWater Research
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Some Features of Polymeric Membranes for Water Purification via Membrane Distillation

2011

Polymeric membranes are currently adopted in water purification processes, such as reverse osmosis (RO) and membrane distillation (MD). This latter technique is very promising for separation effectiveness and energy savings. A valuable and effective MD unit must be equipped with polymeric membranes that exhibit specific properties, for example, hydrophobicity, a narrow pore size range, a high water penetration pressure, and a large vapor permeability. In this work, we present and examine the main features of membranes for MD processes, with the aim of experimentally evaluating the related performances. Scanning electron microscopy analysis was carried out for a first estimate of the pore si…

PolytetrafluoroethylenePolymers and PlasticsChemistryScanning electron microscopeAnalytical chemistrySynthetic membranePortable water purificationGeneral ChemistryPenetration (firestop)Membrane distillationSurfaces Coatings and Filmschemistry.chemical_compoundMembranemorphologyMaterials ChemistryReverse osmosisgas permeationmembrane
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Fate of aerobic granular sludge in the long-term: The role of EPSs on the clogging of granular sludge porosity

2016

This work aims to investigate the stability of aerobic granular sludge in the long term, focusing on the clogging of the granular sludge porosity exerted by the extracellular polymeric substances (EPSs). The effects of different cycle lengths (short and long-term cycle) on the granular sludge stability were investigated. Results obtained outlined that during the short duration cycle, the formation and breakage of the aerobic granules were continuously observed. During this period, the excess of EPS production contributed to the clogging of the granules porosity, causing their breakage in the long run. During the long-duration cycle, the extended famine period entailed a greater EPSs consump…

Porosity cloggingEnvironmental EngineeringPolymers0208 environmental biotechnology02 engineering and technology010501 environmental sciencesManagement Monitoring Policy and Law01 natural sciencesWater PurificationCloggingBioreactorsExtracellular polymeric substanceBreakageExtracellular polymeric substances (EPSs)BiomassLong-term stabilityPorosityWaste Management and DisposalShort durationAerobic granular sludge; Extracellular polymeric substances (EPSs); Feast/famine; Long-term stability; Porosity clogging; Environmental Engineering; Waste Management and Disposal; Management Monitoring Policy and Law0105 earth and related environmental sciencesBacteriaSewageSettore ICAR/03 - Ingegneria Sanitaria-AmbientaleChemistryEnvironmental engineeringGeneral MedicineLimitingPulp and paper industryAerobiosis020801 environmental engineeringFeast/famineAerobic granular sludgePorosity
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