Search results for " purification"
showing 10 items of 244 documents
Optimization of a Biotechnological Process for Production and Purification of Two Recombinant Proteins: Col G and Col H
2017
Different strategies can be used for increasing production of heterologous recombinant proteins in Escherichia coli. Protein size is often critical for obtaining the best quantity/quality ratio of recombinant protein expression. This study focuses on two recombinant proteins; Class I and class II Collagenases, namely Col G and Col H. Their size is about 150 kDa each. We have developed a method to obtain high levels of cell growth and intracellular expression of each Collagenases in recombinant E. coli BL21(DE3). Batch and Fed-batch fermentation procedures have been performed. Results show that Fed-batch technique was most effective in obtaining the highest cell density for each recombinant …
Management of Acute Kidney Injury and Extracorporeal Blood Purification Therapies During the COVID-19 Pandemic: The Italian SIN–SIAARTI Joint Survey …
2022
Background and AimThe novel coronavirus disease 2019 remains challenging. A large number of hospitalized patients are at a high risk of developing AKI. For this reason, we conducted a nationwide survey to assess the incidence and management of AKI in critically ill patients affected by the SARS-CoV-2 infection.MethodsThis is a multicenter, observational, nationwide online survey, involving the Italian Society of Nephrology and the critical care units in Italy, developed in partnership between the scientific societies such as SIN and SIAARTI. Invitations to participate were distributed through emails and social networks. Data were collected for a period of 1 week during the COVID-19 pandemic…
Biological nutrient removal in membrane bioreactors: denitrification and phosphorus removal kinetics.
2007
The impact of including membranes for solid liquid separation on the kinetics of nitrogen and phosphorus removal was investigated. To achieve this, a membrane bioreactor (MBR) biological nutrient removal (BNR) activated sludge system was operated. From batch tests on mixed liquor drawn from the MBR BNR system, denitrification and phosphorus removal rates were delineated. Additionally the influence of the high total suspended solids concentrations present in the MBR BNR system and of the limitation of substrate concentrations on the kinetics was investigated. Moreover the ability of activated sludge in this kind of system to denitrify under anoxic conditions with simultaneous phosphate uptak…
The importance of Hygienics and Clean Water Treatment in Valencia (1860-1910)
2016
El siglo XIX se caracteriza por numerosos cambios tanto a nivel social como estructural de la población: se produce un acelerado desarrollo urbano sin planificación y carente de servicios que da lugar a una mala calidad de vida y numerosas epidemias. Estos cambios tienen como resultado un nuevo movimiento, el Higienismo, que pretende mejorar las condiciones de vida de la población. En España, todas las reformas higiénicas se desarrollaron mediante los planes de Ensanche. Uno de los puntos clave de este pensamiento es el abastecimiento de agua potable como medio para evitar enfermedades. La ciudad de Valencia es un claro ejemplo de la importancia de este movimiento y cómo el abastecimiento d…
Removal of bis (2-ethylhexyl) phthalate from reject water in a nitrogen-removing sequencing batch reactor.
2003
Reject water from sewage sludge processing may contain high concentrations of nutrients and organic pollutants and cause internal pollution load at a sewage treatment plant (STP) if circulated to the headworks of an STP. In the present study removal of nitrogen and bis (2-ethylhexyl) phthalate (DEHP) from reject water was studied in two sequencing batch reactors (SBRs) with different aerobic/anoxic periods during a 6-h total cycle period. Ammonia-nitrogen (NH(4)-N) was almost totally removed in both reactors, apparently by nitrification throughout the run, while denitrification declined with decreasing SCOD in the influent resulting in an increase in the effluent nitrate-nitrogen (NO(3)-N) …
Removal of bis(2-ethylhexyl) phthalate at a sewage treatment plant
2003
Bis(2-ethylhexyl) phthalate (DEHP) concentrations were measured at different stages in a full-scale sewage treatment plant (STP) and mass balances were calculated. The DEHP load to treatment process coming from the sewer system and the internal load comprising returned supernatants and filtrate from sludge treatment and excess secondary sludge were at the same level. The DEHP removal efficiency from the water phase at the STP was on average 94% of sewage DEHP, the main removal process being sorption to primary and secondary sludges. On average 29% of DEHP was calculated to be removed in the biological nitrifying-denitrifying activated sludge process, which was much less than expected from l…
The influence of backslopping on lactic acid bacteria diversity in tarhana fermentation
2020
Tarhana is produced at batch systems in which the microbiota has changed accordingly to the microbial load from ingredients. In order to stabilize the microbiota, the effects of backslopping carried out under different temperature regimes (25 and 30 °C), pH (3.70 and 4.00) and inoculation rates (5, 10 and 15%) on lactic acid bacteria (LAB) diversity were determined in tarhana dough. LAB and Total Aerobic Mesophilic Bacteria (TAMB) numbers increased in all tarhana dough samples subjected to backslopping. Temperature and pH significantly affected the microbiological diversity of tarhana whereas the different inoculation rates did not. Tarhana dough showed complex tarhana microbiota following …
Efficacy of Hydrogen Purification and Cosmetic Acids in the Treatment of Acne Vulgaris: A Preliminary Report
2022
Acne and skin lesions that appear in its course deteriorate the quality of life of patients, cause depression and the emergence of suicidal thoughts. Cosmetic treatments can have a positive effect on improving skin condition by cleaning up skin eruptions, thus improving the well-being of affected people. Hydrogen purification is a treatment that uses alkaline water generated by a device, which reduces sebum from the surface of the epidermis. This is a novel treatment that has recently been introduced to beauty salons. On the other hand, cosmetic acids have been used for many years for treating people with acne vulgaris and give spectacular results in terms of improving the skin condition. I…
The use of hydrogen purification and mixture of cosmetics acids, and the influence on acne skin parameters: a case report
2022
Background: Acne vulgaris is a common disease which causes physical and mental ailments that are associated with a significantly reduced quality of life. Contemporary cosmetology is looking for innovative and effective methods of treating acne vulgaris. The synergistic action of acids and hydrogen purification has a positive effect on the reduction of sebum secretion and thus the amount of skin eruptions. The aim of the study : To check the effectiveness of hydrogen purification treatment combined with topical acids for the treatment of acne vulgaris. Case report: The participant of this study is a 20-year-old female, struggling with acne vulgaris for 5 years. She has a problem with large s…
Acne vulgaris and the most popular and new cosmetological treatments
2023
Introduction: Acne vulgaris is a disease that has an enormous impact on appearance of the skin of the patients and also on their quality of life. Long-term dermatological treatment, which often lasts years, and disease relapses contribute to the occurrence of low self-esteem and depression. Acne is one of the 10 most common diseases in the world and the most common inflammatory skin disease. Many methods of dermatological treatment that bring very good results are available. However, educating people with acne on how to take care of their skin on a daily basis to minimize the bleak effects of the disease is an extremely important element. Contemporary cosmetology creates an environment for …