Search results for " reading"

Partial vinylphenol reductase purification and characterization from Brettanomyces bruxellensis

2008

International audience; Brettanomyces is the major microbial cause for wine spoilage worldwide and causes significant economic losses. The reasons are the production of ethylphenols that lead to an unpleasant taint described as 'phenolic odour'. Despite its economic importance, Brettanomyces has remained poorly studied at the metabolic level. The origin of the ethylphenol results from the conversion of vinylphenols in ethylphenol by Brettanomyces hydroxycinnamate decarboxylase. However, no information is available on the vinylphenol reductase responsible for the conversion of vinylphenols in ethylphenols. In this study, a vinylphenol reductase was partially purified from Brettanomyces bruxe…

Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracel…

2010

Abstract Background A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics. Results One clone with a 40 kb insert showed antimicrobial activity against Gram positive bacteria. Insert sequence analysis and subcloning experiments revealed that the bioactivity was due to a 3.5 kb DNA fragment containing two open reading frames. These orfs encode two proteins with high similarity to a putative membrane protein of Streptomyces coelicolor and to the nogalamycin resis…

Promoting Social and Political Change Through Pedagogy: Lorenzo Milani and the Barbiana School (Italy 1954–1967)

2015

This chapter describes the curriculum and pedagogy implemented at Barbiana, a small school in a remote and mountainous place in Italy. Still revered in Europe as one of the most interesting innovative teaching experiences, the school started in 1954 when Lorenzo Milani, a Catholic priest committed to work with the poor who had run an afterschool program in a working-class parish in an industrial city close to Florence, was sent to this location because of his leftist views. Milani believed that schools were class-based institutions that benefited bourgeois children and that excluded working-class and farmworker students. To reverse this situation, Milani contended that education for these s…

Producing social mobility. Class and Travel in the Romanian Novel 1901-1932

2020

The present article addresses the Romanian novelistic production between 1901 and 1932 in the attempt of identifying a series of patterns regarding the protagonists’ social mobility. Starting with the most mentioned destinations throughout the novels, I analyse how and why the different social classes travel and try to determine the landmarks between which they dispute their physical presence, on the one hand, and their aspirations, on the other. On this basis, the second part of the article conducts a quantitative analysis of the major means of transport in the period – the train, the tramway, the coach/carriage, the automobile, the aeroplane, the ship, and the waggon – and attempts to pin…

Sequencing and analysis of the gene encoding the α-toxin of Clostridium novyi proves its homology to toxins A and B of Clostridium difficile

1995

A library of total Clostridium novyi DNA was established and screened for the alpha-toxin gene (tcn alpha) by hybridization with oligonucleotides derived from a partial N-terminal sequence and by using specific antisera. Overlapping subgenic tcn alpha fragments were isolated and subsequently the total sequence of tcn alpha was determined. The 6534 nucleotide open reading frame encodes a polypeptide of M(r) 250,166 and pI 5.9. The N-terminal alpha-toxin (Tcn alpha) sequence MLITREQLMKIASIP determined by Edman degradation confirmed the identity of the reading frame and the assignment of the translation start point. The toxin is not modified posttranslationally at its N-terminus nor does it co…

GenClust: A genetic algorithm for clustering gene expression data

2005

Abstract Background Clustering is a key step in the analysis of gene expression data, and in fact, many classical clustering algorithms are used, or more innovative ones have been designed and validated for the task. Despite the widespread use of artificial intelligence techniques in bioinformatics and, more generally, data analysis, there are very few clustering algorithms based on the genetic paradigm, yet that paradigm has great potential in finding good heuristic solutions to a difficult optimization problem such as clustering. Results GenClust is a new genetic algorithm for clustering gene expression data. It has two key features: (a) a novel coding of the search space that is simple, …

Prediction of the difficulty level in a standardized reading comprehension test: contributions from cognitive psychology and psychometrics

2013

This research seeks to identify possible predictors of the difficulty level of reading comprehension items used in a standardized psychometric test for university admission. Several potential predictors of difficulty were proposed, namely, propositional density, negations, grammatical structure, vocabulary difficulty, presence of enhancement elements (words highlighted typographically), item abstraction level and degree of similarity between correct option and relevant text to resolve the item. By Linear Logistic Test Model (Fisher, 1973) it was found that the number of propositions, the syntactic structure, and fundamentally, the presence of difficult words contributed to the prediction of…

The soundslide report : innovative journalism or misplaced works of art?

2014

Published version of an article in the journal: Nordicom Review. Also available from the publisher at: http://dx.doi.org/10.2478/nor-2014-0007 Open Access The audio slideshow-or soundslide report-represents a new format for journalistic reporting on online news sites. It is not very widely used, but it has certain discursive and aesthetic potentials indicating that it could contribute substantially to the ecology of journalistic genres. The article offers an illustration and discussion of these potentials, asking how the format communicates and how it affects journalism in general. Starting out with a close reading of a sample text and a discussion of the format's position in a network of g…

Recensione di Carolina Montagni. Come promuovere le raccolte in biblioteca. Milano: Editrice Bibliografica, 2018. 80 p. (Library toolbox; 27) e Maria…

2019

Blending Literature and Foreign Language Learning: Current Approaches

2013

The aim of this chapter is to discuss the place of literature in foreign language learning and teaching contexts, and to show reasons of using literary texts for the development of communicative competence, intercultural communicative competence, and for individual as well as social human growth. Although literature and language teaching had been following separate paths, currently a strong tendency emerges of integrating language and literature teaching across proficiency levels. This tendency results from the recognition of the roles that literacy, multiliteracies and multimodality play in the life of humans in the 21st century. Respected bodies such as the Council of Europe or Modern Lan…