Search results for " regulatory"
showing 10 items of 556 documents
Smad7 controls resistance of colitogenic T cells to regulatory T cell-mediated suppression.
2008
Background & Aims Foxp3-expressing regulatory T cells (Tregs) play a key role in the maintenance of the gut immune homeostasis, and an intact transforming growth factor (TGF)-β signaling is required for their function. In inflammatory bowel disease (IBD), the TGF-β signaling is impaired because of high expression of the inhibitory molecule Smad7. Although no intrinsic defects in Tregs function have been shown in IBD, it is still unknown whether colitogenic T cells are susceptible to Treg-mediated suppression. In this study, we have investigated whether IBD mucosal CD4+ T cells are resistant to Tregs and whether Smad7 is involved in this process. Methods IBD lamina propria mononuclear cells …
L'attività normativa del governo nel periodo 2017-2019
2022
L’articolo descrive l’attività normativa del governo nel periodo 2017-2019. Per quanto riguarda le procedure di programmazione e revisione della spesa pubblica, le riforme del 2016 finalizzate alla riduzione della spesa dei Ministeri hanno trovato applicazione solo nel 2017, mentre nel 2018 e nel 2019 ulteriori risparmi sono stati conseguiti mediante apposite previsioni delle leggi di bilancio. Le principali innovazioni in materia di liberalizzazione sono state portate a termine nel 2017, mentre non vi sono stati interventi rilevanti nel biennio successivo. Per quanto concerne il decentramento di funzioni amministrative, non si sono riscontrati progressi. Le politiche di privatizzazione son…
Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C
2020
Macroautophagy is a conserved degradative process for maintaining cellular homeostasis and plays a key role in aging and various human disorders. The microtubule-associated protein 1A/1B light chain 3B (MAP1LC3B or LC3B) is commonly analyzed as a key marker for autophagosomes and as a proxy for autophagic flux. Three paralogues of the LC3 gene exist in humans: LC3A, LC3B and LC3C. The molecular function, regulation and cellular localization of LC3A and LC3C have not been investigated frequently, even if a similar function to that described for LC3B appears likely. Here, we have selectively decapacitated LC3B by three separate strategies in primary human fibroblasts and analyzed the evoked e…
New techs, new threats: sfide e opportunità della rivoluzione blockchain
2021
Considering the current diffusion of blockchain technology in the most different fields, ranging from public governance to economics, from contractual applications to health, the contribution addresses the issue of the legal regulation of this new technology, precisely because of its vast applications. From the comparison between the European model and the American one, the approach of the European legislator ‒ despite the delay with which the institutions have addressed the issue, seems to show, as already happened in other areas, a more 'social oriented' perspective and based on a sort of 'democratization' of data compared to the American approach, more ‘business oriented’, where the scho…
Genetic and Molecular Characterization of The Human Osteosarcoma 3AB-OS Cancer Stem Cell Line: A Possible Model For Studying Osteosarcoma Origin and …
2013
Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical to halt cancer and improve patient survival. Osteosarcoma is an aggressive tumor affecting adolescents, for which there is no second-line chemotherapy. Uncovering new molecular mechanisms underlying the development of osteosarcoma and origin of CSCs is crucial to identify new possible therapeutic strategies. Here, we aimed to characterize genetically and molecularly the human osteosarcoma 3AB-OS CSC line, previously selected from MG63 cells and which proved to have both in vitro and in vivo features of CSCs. Classic cytogenetic studies demonstrated that 3AB-OS cells have hypertriploid karyotype wit…
Regulation of Farnesyl Diphosphate Synthase Gene Expression by Fatty Acids
2003
Cholesterol biosynthesis depends on the activity of regulatory enzymes, including the peroxisomal Farnesyl Diphosphate Synthase (FPPS ). Cholesterol regulates its own synthesis rate. Hence, as a response to cholesterol depletion, a feed back mechanism is activated, whereby sterol regulatory binding proteins (SREBPla, 1c and 2 ) are subjected to sequential proteolytic activation, which permits their interaction with specific DNA response elements from responsive genes. In turn, the transcriptional activity of cholesterol biosynthesis genes is induced. Conversely, cholesterol accumulation decreases SREBP maturation and transcription of controlled genes. In addition, polyunsaturated fatty acid…
Regulation of T-Cell Immune Responses by Pro-Resolving Lipid Mediators
2021
Both the initiation and the resolution of inflammatory responses are governed by the sequential activation, migration, and control/suppression of immune cells at the site of injury. Bioactive lipids play a major role in the fine-tuning of this dynamic process in a timely manner. During inflammation and its resolution, polymorphonuclear cells (PMNs) and macrophages switch from producing pro-inflammatory prostaglandins and leukotrienes to specialized pro-resolving lipid mediators (SPMs), namely, lipoxins, resolvins, protectins, and maresins, which are operative at the local level to limit further inflammation and tissue injury and restore homeostasis. Accumulating evidences expand now the rol…
Revindication of religious organizations’ properties in Poland : Thirty years’ experience of building a democratic state ruled by law
2022
Celem tego artykułu jest analiza przepisów, na mocy których odbywa się w Polsce po 1989 r. rewindykacja nieruchomości osób prawnych związków wyznaniowych upaństwowionych w okresie Polski Ludowej. Omówiono w nim zarówno tryby, jak i sposoby regulacji spraw majątkowych wyznaniowych osób prawnych, uwzględniając liczne zmiany stanu prawnego wprowadzane kolejnymi nowelizacjami przepisów oraz ewolucję ich wykładni dokonywanej przez judykaturę i doktrynę. Zwrócono szczególną uwagę na dyskusyjne kwestie prawne, w tym na nieuprawnione zróżnicowanie sytuacji prawnej poszczególnych związków wyznaniowych. Przeprowadzone w artykule analizy prowadzą do wniosku, że ustawodawca konsekwentnie uwłaszczył wsz…
L‐Aspartate as a high‐quality nitrogen source in Escherichia coli : Regulation of L‐aspartase by the nitrogen regulatory system and interaction of L‐…
2020
Escherichia coli uses the C4-dicarboxylate transporter DcuA for L-aspartate/fumarate antiport, which results in the exploitation of L-aspartate for fumarate respiration under anaerobic conditions and for nitrogen assimilation under aerobic and anaerobic conditions. L-Aspartate represents a high-quality nitrogen source for assimilation. Nitrogen assimilation from L-aspartate required DcuA, and aspartase AspA to release ammonia. Ammonia is able to provide by established pathways the complete set of intracellular precursors (ammonia, L-aspartate, L-glutamate, and L-glutamine) for synthesizing amino acids, nucleotides, and amino sugars. AspA was regulated by a central regulator of nitrogen meta…
Inferring slowly changing dynamic gene-regulatory networks
2014
Dynamic gene-regulatory networks are complex since the interaction patterns between its components mean that it is impossible to study parts of the network in separation. This holistic character of gene-regulatory networks poses a real challenge to any type of modelling. Graphical models are a class of models that connect the network with a conditional independence relationships between the random variables. By interpreting the random variables as gene activities and the conditional independence relationships as functional non-relatedness, graphical models have been used to describe gene-regulatory networks. Whereas the literature has been focused on static networks, most time-course experi…