Search results for " roots"

showing 10 items of 286 documents

Triterpene saponins from Eryngium kotschyi

2015

Four new oleanane-type saponins 3-O-alpha-L-rhamnopyranosyl-(1 -> 4)-beta-D-glucuronopyranosyl-22-O-beta, beta-dimethylacryloylA1-barrigenol (1), 3-O-alpha-L-rhamnopyranosyl-(1 -> 4)-beta-D-glucuronopyranosyl-22-O-angeloylA1-barrigenol (2), 3-O-beta-D-glucopyranosyl-(1 -> 2)-[beta-D-glucopyranosyl-(1 -> 6)]-beta-D-glucopyranosyl-21,22,28-O-triacetyl-(3 beta,21 beta,22 alpha)-olean-12-en-16-one (3), and 3-O-beta-D-glucopyranosyl-(1 -> 2)-glucopyranosyl-22-O-beta-D-glucopyranosylsteganogenin (4), along with the known 3-O-beta-D-galactopyranosyl-(1 -> 2)-[alpha-L-arabinopyranosyl-(1 -> 3)]-beta-D-glucuronopyranosyl-22-O-angeloylA1-barrigenol and 3-O-alpha-L-rhamnopyranosyl-(1 -> 4)-beta-D-gluc…

chemistry.chemical_classificationApiaceaeKetoneMolecular StructureTurkeybiologyEryngiumStereochemistryStereoisomerismPlant ScienceGeneral MedicineSaponinsHorticulturebiology.organism_classificationPlant RootsBiochemistryEryngium kotschyichemistryTriterpeneOleanolic AcidNuclear Magnetic Resonance BiomolecularMolecular BiologyTwo-dimensional nuclear magnetic resonance spectroscopy
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Two new triterpene saponins from Eryngium campestre.

2005

Two new triterpene saponins, 3-O-beta-D-glucopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucuronopyranosyl-22-O-angeloyl-R1-barrigenol (1) and 3-O-beta-D-glucopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucuronopyranosyl-22-O-beta,beta-dimethylacryloyl-A1-barrigenol (2), were isolated from the roots of Eryngium campestre (Apiaceae). Their structures were established mainly by 2D NMR techniques and mass spectrometry.

chemistry.chemical_classificationApiaceaeMagnetic Resonance SpectroscopybiologyStereochemistryMolecular ConformationGeneral ChemistryGeneral MedicineReference StandardsSaponinsbiology.organism_classificationMass spectrometryPlant RootsMolecular conformationMass SpectrometryTriterpenesEryngium campestreTriterpenechemistryDrug DiscoveryTwo-dimensional nuclear magnetic resonance spectroscopyReference standardsApiaceaeChemicalpharmaceutical bulletin
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Factors influencing axillary shoot proliferation and adventitious budding in cedar.

2005

We developed procedures for in vitro cloning of Cedrus atlantica Manetti and C. libani A. Rich explants from juvenile and mature plants. Explant size was one determinant of the frequency of axillary bud break in both species. Shoot tips and nodal explants mainly developed calli, whereas bud sprouting occurred in defoliated microcuttings cultured on a modified Murashige and Skoog medium without growth regulators. Isolation and continuous subculture of sprouted buds on the same medium allowed cloning of microcuttings from C. atlantica and C. libani seedlings and bicentennial C. libani trees, thus providing a desirable alternative for multiplying mature trees that have demonstrated superior ch…

chemistry.chemical_classificationBuddingbiologyPhysiologyCedrus atlanticaTemperaturePlant ScienceCedrus libanibiology.organism_classificationPlant RootsTissue Culture TechniquesMurashige and Skoog mediumchemistryPlant Growth RegulatorsAuxinAxillary budShootBotanySeedsCedrusPlant ShootsExplant cultureTree physiology
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A new oleanane glycoside from the roots ofAstragalus caprinus

2006

A novel oleanane-type triterpene saponin (1) together with two known molecules, soyasapogenol B and astragaloside VIII were isolated from the roots of Astragalus caprinus. Their structural elucidation was performed mainly by 2D NMR techniques (COSY, TOCSY, NOESY, HSQC, HMBC) and mass spectrometry. Compound 1 was determined as 3-O-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucuronopyranosyl]-22-O-beta-D-apiofuranosyl-soyasapogenol B.

chemistry.chemical_classificationMagnetic Resonance SpectroscopyMolecular StructureChemistryStereochemistrySaponinGlycosideAstragalus PlantGeneral ChemistryNuclear magnetic resonance spectroscopyPlant Rootschemistry.chemical_compoundAstragalosideTriterpeneOrganic chemistryGeneral Materials ScienceGlycosidesOleanolic AcidOleananeTwo-dimensional nuclear magnetic resonance spectroscopyHeteronuclear single quantum coherence spectroscopyMagnetic Resonance in Chemistry
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Acylated triterpene saponins from the roots of Securidaca longepedunculata.

2009

Abstract Four triterpene saponins , 3- O -β- d -glucopyranosylpresenegenin 28- O -β- d -apiofuranosyl-(1 → 3)-β- d -xylopyranosyl-(1 → 4)-[β- d -apiofuranosyl-(1 → 3)]-α- l -rhamnopyranosyl-(1 → 2)-{4- O -[( E )-3,4,5-trimethoxycinnamoyl]}-β- d -fucopyranosyl ester, 3- O -β- d -glucopyranosylpresenegenin 28- O -β- d -apiofuranosyl-(1 → 3)-β- d -xylopyranosyl-(1 → 4)-[β- d -apiofuranosyl-(1 → 3)]-α- l -rhamnopyranosyl-(1 → 2)-[(6- O -acetyl)-β- d -glucopyranosyl-(1 → 3)]-{4- O -[( E )-3,4,5-trimethoxycinnamoyl]}-β- d -fucopyranosyl ester, 3- O -β- d -glucopyranosylpresenegenin 28- O -β- d -apiofuranosyl-(1 → 3)-β- d -xylopyranosyl-(1 → 4)-[β- d -apiofuranosyl-(1 → 3)]-α- l -rhamnopyranosyl-(…

chemistry.chemical_classificationMolecular StructureChemistryStereochemistryPlant ExtractsChemical structureAcylationSaponinSecuridacaPlant ScienceGeneral MedicineHorticultureSaponinsBiochemistryPresenegeninPlant RootsTriterpenesTriterpenoidSecuridaca longepedunculataTriterpeneMolecular BiologyTwo-dimensional nuclear magnetic resonance spectroscopyPhytochemistry
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A new dibenzofuran and other constituents from Ligularia caloxantha, a Chinese medicinal plant.

2008

A new dibenzofuran named 1,2,4-trimethyl-7,8-dimethoxy-dibenzofuran (1), together with seven known compounds, euparin (2), 2,5-diacetyl-6-hydroxy-benzofuran (3), 2-acetyl-5,6-dimethoxy-benzofuran (4), gummosogenin (5), lupeol (6), stigmasterol (7) and (E)-2,5-dihydroxy-cinnamic acid (8), were isolated from the roots of Ligularia caloxantha, a Chinese medicinal plant. The structures of the compounds were elucidated by spectroscopic methods.

chemistry.chemical_classificationStigmasterolMolecular StructureChemistryOrganic ChemistryPlant ScienceLigularia caloxanthaAsteraceaeBiochemistryPlant RootsAnalytical ChemistryDibenzofuranchemistry.chemical_compoundTriterpeneOrganic chemistryBenzofuranLupeolBenzofuransDrugs Chinese HerbalNatural product research
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STUDY ON CONVERSION OF ENCAPSULATED VITRO-DERIVED PROPAGULES OF 'C35' CITRANGE [C. SINENSIS (L.) OSB. × P. TRIFOLIATE (L.) RAF.]

2015

Fight against Citrus tristeza virus (CTV) implies the use of CTV-resistant rootstocks, such as citranges [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]. The 'C35' represents a promising tolerant to CTV genotype. The employment of innovative in vitro techniques, such as encapsulation, could open new perspectives for this genotype propagation, particularly in the nursery activity. Preliminary experiments on calcium-alginate encapsulation of microcuttings (unipolar propagules) have already been performed to evaluate the use of tissue culture for the propagation and the preservation of different citrange rootstocks: Carrizo and Troyer. In the present study, the influence of different types a…

chemistry.chemical_classificationmicropropagationEncapsulated microcuttingHorticulturePlant disease resistanceBiologybiology.organism_classificationCitrangeSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeTissue cultureHorticulturePropagulechemistryMicropropagationAuxinPlant virusCitrus rootstock encapsulated microcuttings micropropagationRootstockCitrus rootstockencapsulated microcuttingsActa Horticulturae
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Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

2009

Natural disease-suppressive soils provide an untapped resource for the discovery of novel beneficial microorganisms and traits. For most suppressive soils, however, the consortia of microorganisms and mechanisms involved in pathogen control are unknown. To date, soil suppressiveness to Fusarium wilt disease has been ascribed to carbon and iron competition between pathogenic Fusarium oxysporum and resident non-pathogenic F. oxysporum and fluorescent pseudomonads. In this study, the role of bacterial antibiosis in Fusarium wilt suppressiveness was assessed by comparing the densities, diversity and activity of fluorescent Pseudomonas species producing 2,4-diacetylphloroglucinol (DAPG) (phlD+) …

chlororaphis pcl1391Antifungal AgentsDISEASE SUPRESSIVE SOILMicroorganismColony Count Microbialdose-response relationshipsFLUORESCENT PSEUDOMONADSblack root-rotPlant Rootsgraminis var triticiFusariumSolanum lycopersicumFlaxCluster AnalysisFUSARIUM WILTPathogenPhylogenySoil Microbiologymedia_commonEcologyEPS-2genotypic diversityfood and beveragesBiodiversitygenetic diversityFusarium wilt[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyPHENAZINE ANTIBIOTICSPolymorphism Restriction Fragment LengthDNA BacterialGenotypemedia_common.quotation_subject2PhloroglucinolBiologyMicrobiologyCompetition (biology)MicrobiologyPseudomonasAntibiosisBotanyFusarium oxysporumEcology Evolution Behavior and Systematicsbiological-controlAntibiosisbiology.organism_classificationLaboratorium voor PhytopathologieLaboratory of Phytopathology24-diacetylphloroglucinol-producing pseudomonasoxysporum fo47PhenazinesBeneficial organismAntagonism4-diacetylphloroglucinol-producing pseudomonasnonpathogenic fusarium
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Antimicrobial and Antioxidant Activities of Coumarins from the Roots of Ferulago campestris (Apiaceae)

2009

We report the isolation of several coumarins and the stereochemical assessment of some pyranocoumarins, as well as the antibacterial and antioxidant activities of the three most abundant ones (grandivittin, agasyllin and aegelinol benzoate) isolated from the roots of Ferulago campestris collected in Sicily and of the hydrolysis product (aegelinol). Aegelinol and agasyllin showed antibacterial activity against nine ATCC and the same clinically isolated Gram-positive and Gram-negative bacterial strains. At a concentration between 16 and 125 μg/mL both coumarins showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. In particular the ATCC strains Staph…

food.ingredientNeutrophilsPharmaceutical ScienceBiologyGram-Positive Bacteriamedicine.disease_causePlant RootsPyranocoumarinsPyranocoumarinsAntioxidantsArticleAnalytical ChemistryFerulagoMicrobiologyfoodAnti-Infective AgentsAntioxidant activityCoumarinsGram-Negative BacteriaDrug DiscoveryLeukocytesmedicineHumansAbsolute configurationPhysical and Theoretical ChemistryFerulago campestris coumarins pyranocoumarins absolute configuration antibacterial activity antioxidant activityDose-Response Relationship DrugOrganic ChemistrySettore CHIM/06 - Chimica OrganicaEnterobacterbiology.organism_classificationAntimicrobialChemistry (miscellaneous)Staphylococcus aureusMolecular MedicineFerulago campestris; Coumarins; Pyranocoumarins; Absolute configuration; Antibacterial activity; Antioxidant activityFerulago campestrisAntibacterial activityAntibacterial activityEnterobacter cloacaeBacteriaApiaceaeMolecules; Volume 14; Issue 3; Pages: 939-952
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Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR

2011

Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen (R) method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affec…

fungal abundance organic carbon content real-time Q-PCR length polymorphism SYBRGreen method type de sol[SDV]Life Sciences [q-bio]lcsh:MedicinePlant SciencePlant Roots18S ribosomal RNASYBRGreen methodtype de sol[ SDE ] Environmental SciencesSoilFungal Reproductionlcsh:ScienceDNA FungalPhylogenyorganic carbon content2. Zero hunger0303 health sciencesDiversityMultidisciplinaryfungal abundanceEcologyEcologyRevealsFungal geneticsPolymerase-chain-reactionAgricultureBiodiversityAmpliconSoil Ecologysoil texture amplification enzymatique de l'adnBacterial communitiesSamplesreal-time Q-PCRCommunity Ecology[SDE]Environmental SciencesRhizosphereResearch ArticleSoil textureIn silicoMolecular Sequence DataSoil ScienceComputational biologyMycologyBiologyReal-Time Polymerase Chain ReactionMicrobiologyMicrobial Ecology03 medical and health sciencesSpecies SpecificityMedicago truncatulaMicrobial communityRNA Ribosomal 18SSoil ecologyBiology030304 developmental biologyDNA PrimersRibosomal-Rna genes[ SDV ] Life Sciences [q-bio]030306 microbiologylcsh:RFungiBotanyReproducibility of Resultslength polymorphismsoil textureSequence Analysis DNADna15. Life on landamplification enzymatique de l'adnDNA extractionlcsh:QPrimer (molecular biology)
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