Search results for " secondary"

Vegetation effects on cross-sectional flow in a large amplitude meandering bend

2017

ABSTRACTCross-stream circulation, which develops in meandering bends, exerts an important role in velocity redistribution. This paper investigates how the presence of vegetation could affect the evolution pattern of cross-stream flow along a high-curvature meandering bend. The analysis is conducted with the aid of data collected in a meandering laboratory flume over non-vegetated and vegetated beds. The experiments reveal that, once the vegetation is introduced, the flow pattern determined by the channel’s curvature is interrupted. In the presence of vegetation, the central-region circulation cell seems to be divided into thin circulation cells developing at the top of the vegetated layer a…

Factors Affecting Polyphenol Biosynthesis in Wild and Field Grown St. John’s Wort (Hypericum perforatum L. Hypericaceae/Guttiferae)

2009

The increasing diffusion of herbal products is posing new questions: why are products so often different in their composition and efficacy? Which approach is more suitable to increase the biochemical productivity of medicinal plants with large-scale, low-cost solutions? Can the phytochemical profile of a medicinal plant be modulated in order to increase the accumulation of its most valuable constituents? Will polyphenol-rich medicinal crops ever be traded as commodities? Providing a proactive answer to such questions is an extremely hard task, due to the large number of variables involved: intraspecific chemodiversity, plant breeding, ontogenetic stage, post-harvest handling, biotic and abi…

Cell surface display of rat invariant γ chain: detection by monoclonal antibodies directed against a C-terminal γ chain segment

1992

A series of 14 monoclonal antibodies (mAb) directed against the C-terminal part of the rat invariant gamma chain (amino acid 142-216) was generated using distinct fusion proteins that contain this gamma segment for immunization and hybridoma screening. Additional fusion protein were prepared carrying discrete regions of the gamma chain. Employing these reagents confirmed that the obtained mAb do indeed recognize the C-terminal portion of the invariant chain, as demonstrated by Western blot analysis. All mAb established recognize epitopes present on the native gamma chain, as revealed by immunoprecipitation analysis using nonionic detergent extracts of metabolically labeled Lewis rat splenoc…

Self-Organization Pathways and Spatial Heterogeneity in Insulin Amyloid Fibril Formation

2009

At high temperature and low pH, the protein hormone insulin is highly prone to form amyloid fibrils, and for this reason it is widely used as a model system to study fibril formation mechanisms. In this work, we focused on insulin aggregation mechanisms occurring in HCl solutions (pH 1.6) at 60 degrees C. By means of in situ Thioflavin T (ThT) staining, the kinetics profiles were characterized as a function of the protein concentration, and two concurrent aggregation pathways were pointed out, being concentration dependent. In correspondence to these pathways, different morphologies of self-assembled protein molecules were detected by atomic force microscopy images also evidencing the prese…

Teacher Training in Linguistic Diversity within Inclusive Education: Cooperation between Non-governmental Non-profit Organizations and Educational In…

2020

Esta tesis examina la colaboración entre las las organizaciones no gubernamentales sin ánimo de lucro centradas en los alumnos con una lengua materna diferente y los centros escolares de tres zonas geográficas europeas: República Checa, Italia y España. Responde a tres preguntas de investigación: (1) ¿Qué servicios ofrecen las organizaciones sin ánimo de lucro a los profesores? (2) ¿Cómo ven las organizaciones no lucrativas la cooperación actual? (3) ¿Conocen los profesores la existencia de organizaciones sin ánimo de lucro en este ámbito y están interesados en dicha cooperación? Se emplea un enfoque de métodos mixtos, aplicando un cuestionario a 34 organizaciones sin ánimo de lucro (9 chec…

Bioconjugates of 1’-Aminoferrocene-1-carboxylic Acid with (S)-3-Amino-2-methylpropanoic Acid and L-Alanine

2010

Formal CH 2 insertion in bioconjugates composed of 1'-aminoferrocene-1-carboxylic acid (Fca) and alanine Boc-Ala-Fca-Ala-OCH 3 gives Fca bioconjugates with the β-amino acid (S)-3-amino-2-methylpropanoic acid (Aib). The novel homologous conjugates of ferrocene were fully characterized by spectroscopic and analytical methods. NMR, CD and IR spectroscopy in concert with DFT calculations suggest that the formal "L-Ala-to-(S)-β-Aib mutations" can exert ferrocene helix inversion due to the different stereogenic carbon atoms of L -Ala and (S)-β-Aib. Furthermore, the mutation (de-)stabilizes the conserved secondary structure with two intramolecular hydrogen bonds, depending on the "mutation site". …

MMGBSA As a Tool To Understand the Binding Affinities of Filamin–Peptide Interactions

2013

Filamins (FLN) are large dimeric proteins that cross-link actin and work as important scaffolds in human cells. FLNs consist of an N-terminal actin-binding domain followed by 24 immunoglobulin-like domains (FLN1-24). FLN domains are divided into four subgroups based on their amino acid sequences. One of these subgroups, including domains 4, 9, 12, 17, 19, 21, and 23, shares a similar ligand-binding site between the β strands C and D. Several proteins, such as integrins β2 and β7, glycoprotein Ibα (GPIbα), and migfilin, have been shown to bind to this site. Here, we computationally estimated the binding free energies of filamin A (FLNa) subunits with bound peptides using the molecular mechan…

Understanding idioms : a comparison of Finnish third grade students of national senior secondary school and IB Diploma Programme

2006

Diagnosis and therapeutic management of primary headache in an emergency setting

2013

Some characterizations of algebras with involution with polynomial growth of their codimensions

2018

Let A be an associative algebra endowed with an involution ∗ of the first kind and let c ∗n (A) denote the sequence of ∗-codimensions of A. In this paper, we are interested in algebras with involution such that the ∗-codimension sequence is polynomially bounded. We shall prove that A is of this kind if and only if it satisfies the same identities of a finite direct sum of finite dimensional algebras with involution A i , each of which with Jacobson radical of codimension less than or equal to one in A i . We shall also relate the condition of having polynomial codimension growth with the sequence of cocharacters and with the sequence of colengths. Along the way, we shall show that the multi…