Search results for " sequence"
showing 10 items of 3643 documents
Parathyroid hormone-related peptide and 8701-BC breast cancer cell growth and invasion in vitro: evidence for growth-inhibiting and invasion-promotin…
1995
It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone-related peptide (PTHrP) gene and that N-terminal PTHrP immunoreactivity can be found in cell medium. Here we have firstly measured immunoreactive PTHrP in 8701-BC cell medium using antibodies raised against midregion and C-terminal fragments, and also demonstrated the expression of PTH/PTHrP receptor by 8701-BC cells. Secondly, we have examined the role, if any, elicited by diverse PTHrP domains on 8701-BC cell proliferation, and invasive behaviour in vitro related to production of extracellular proteolytic enzymes. Our data show that PTHrP [1-34], a…
AB Dor A VLBI images
2020
The fast rotator, pre-main sequence star AB Dor A is a strong and persistent radio emitter. The extraordinary coronal flaring activity is thought to be the origin of compact radio emission and other associated phenomena, such as large slingshot prominences. We aim to investigate the radio emission mechanism and the milliarcsecond radio structure around AB Dor A. We performed phase-referenced VLBI observations at 22.3GHz, 8.4GHz, and 1.4GHz over more than one decade using the Australian VLBI array. Our 8.4GHz images show a double core-halo morphology, similar at all epochs, with emission extending at heights between 5 and 18 stellar radii. Furthermore, the sequence of the 8.4GHz maps shows a…
Evidence for Positive Selection in the Capsid Protein-Coding Region of the Foot-and-Mouth Disease Virus (FMDV) Subjected to Experimental Passage Regi…
2001
We present sequence data from two genomic regions of foot-and-mouth disease virus (FMDV) subjected to several experimental passage regimens. Maximum-likelihood estimates of the nonsynonymous-to-synonymous rate ratio parameter (dN/dS) suggested the action of positive selection on some antigenic sites of the FMDV capsid during some experimental passages. These antigenic sites showed an accumulation of convergent amino acid replacements during massive serial cytolytic passages and also in persistent infections of FMDV in cell culture. This accumulation was most significant at the antigenic site A (the G-H loop of capsid VP1), which includes an Arg-Gly-Asp (RGD) cellular recognition motif. Our …
Partnership formation and dissolution over the life course: applying sequence analysis and event history analysis in the study of recurrent events
2015
We present two types of approach to the analysis of recurrent events for discretely measured data, and show how these methods can complement each other when analysing co-residential partnership histories. Sequence analysis is a descriptive tool that gives an overall picture of the data and helps to find typical and atypical patterns in histories. Event history analysis is used to make conclusions about the effects of covariates on the timing and duration of the partnerships. As a substantive question, we studied how family background and childhood socio-emotional characteristics were related to later partnership formation and stability in a Finnish cohort born in 1959. We found that high se…
Induction of body weight loss through RNAi-knockdown of APOBEC1 gene expression in transgenic rabbits
2014
In the search of new strategies to fight against obesity, we targeted a gene pathway involved in energy uptake. We have thus investigated the APOB mRNA editing protein (APOBEC1) gene pathway that is involved in fat absorption in the intestine. The APOB gene encodes two proteins, APOB100 and APOB48, via the editing of a single nucleotide in the APOB mRNA by the APOBEC1 enzyme. The APOB48 protein is mandatory for the synthesis of chylomicrons by intestinal cells to transport dietary lipids and cholesterol. We produced transgenic rabbits expressing permanently and ubiquitously a small hairpin RNA targeting the rabbit APOBEC1 mRNA. These rabbits exhibited a moderately but significantly reduced …
Early evolutionary colocalization of the nuclear ribosomal 5S and 45S gene families in seed plants: evidence from the living fossil gymnosperm Ginkgo…
2012
In seed plants, the colocalization of the 5S loci within the intergenic spacer (IGS) of the nuclear 45S tandem units is restricted to the phylogenetically derived Asteraceae family. However, fluorescent in situ hybridization (FISH) colocalization of both multigene families has also been observed in other unrelated seed plant lineages. Previous work has identified colocalization of 45S and 5S loci in Ginkgo biloba using FISH, but these observations have not been confirmed recently by sequencing a 1.8 kb IGS. In this work, we report the presence of the 45S–5S linkage in G. biloba, suggesting that in seed plants the molecular events leading to the restructuring of the ribosomal loci are much o…
Characterization and transcription studies of a phytochelatin synthase gene from the solitary tunicate Ciona intestinalis exposed to cadmium.
2014
The major thiol-containing molecules involved in controlling the level of intracellular ROS in eukaryotes, acting as a nonenzymatic detoxification system, are metallothioneins (MTs), glutathione (GSH) and phytochelatins (PCs). Both MTs and GSH are well-known in the animal kingdom. PC was considered a prerogative of the plant kingdom but, in 2001, a phytochelatin synthase (PCS) gene was described in the nematode Caenorhabditis elegans; additional genes encoding this enzyme were later described in the earthworm Eisenia fetida and in the parasitic nematode Schistosoma mansoni but scanty data are available, up to now, for Deuterostomes. Here, we describe the molecular characteristics and transc…
La influencia del peso y la talla en la erupción de la dentición temporal
2017
Objetivos: investigar la cronología y la secuencia de erupción de la dentición temporal y su relación con el peso y la talla en un grupo de niños y niñas de entre 0 y 3 años tanto al nacer como en la exploración. Analizar si existen diferencias significativas en la edad de erupción entre dientes contra laterales y entre dientes antagonistas para ambos sexos. Metodología: La muestra recogida fue constituida por 489 niños de raza caucásica, de los cuales 236 eran niños (48.3%) y 253 eran niñas (51.7%), asignados a dos centros de salud, seleccionados al azar. Todos los niños eran nacidos a término y tenían una edad comprendida entre 3 meses y 36 meses de edad. Ninguno de ellos presentaba alter…
Genome degeneration and adaptation in a nascent stage of symbiosis
2014
Symbiotic associations between animals and microbes are ubiquitous in nature, with an estimated 15% of all insect species harboring intracellular bacterial symbionts. Most bacterial symbionts share many genomic features including small genomes, nucleotide composition bias, high coding density, and a paucity of mobile DNA, consistent with long-term host association. In this study, we focus on the early stages of genome degeneration in a recently derived insect-bacterial mutualistic intracellular association. We present the complete genome sequence and annotation of Sitophilus oryzae primary endosymbiont (SOPE). We also present the finished genome sequence and annotation of strain HS, a close…
Rac1-Regulated Endothelial Radiation Response Stimulates Extravasation and Metastasis That Can Be Blocked by HMG-CoA Reductase Inhibitors
2011
Radiotherapy (RT) plays a key role in cancer treatment. Although the benefit of ionizing radiation (IR) is well established, some findings raise the possibility that irradiation of the primary tumor not only triggers a killing response but also increases the metastatic potential of surviving tumor cells. Here we addressed the question of whether irradiation of normal cells outside of the primary tumor augments metastasis by stimulating the extravasation of circulating tumor cells. We show that IR exposure of human endothelial cells (EC), tumor cells (TC) or both increases TC-EC adhesion in vitro. IR-stimulated TC-EC adhesion was blocked by the HMG-CoA reductase inhibitor lovastatin. Glycyrr…