Search results for " spore"

showing 10 items of 20 documents

TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2).

2016

In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain. The capability of this mutant to grow on minimal medium was rescued by tryptophan and tryptophan precursor (serine and/or indole) su…

Proteomics0301 basic medicineProtein ExtractionMutantlcsh:MedicineStreptomyces coelicolor A3(2)Settore BIO/19 - Microbiologia GeneraleBiochemistrySerinechemistry.chemical_compoundAromatic Amino AcidsSmall ProteinAntibioticsTRPMMicrobial PhysiologyMedicine and Health SciencesBacterial PhysiologyAmino Acidslcsh:ScienceProtein MetabolismExtraction TechniquesMultidisciplinarybiologyOrganic CompoundsAntimicrobialsStreptomyces coelicolorTryptophanDrugsChemistryBiochemistryPhysical SciencesPhysiological DifferentiationResearch ArticleTryptophan BiosynthesiSmall Protein; Biosynthesis; Morpho-Physiological Differentiation: Streptomyces coelicolorBiosynthesisResearch and Analysis MethodsMicrobiologyStreptomycesActinorhodin03 medical and health sciencesBiosynthesisMicrobial ControlBacterial SporesPharmacology030102 biochemistry & molecular biologyOrganic Chemistrylcsh:RChemical CompoundsTryptophanTrpM; Small Protein; Tryptophan Biosynthesis; Morphological Differentiation; Physiological Differentiation; Streptomyces coelicolor A3(2); ProteomicsBiology and Life SciencesProteinsBacteriologybiology.organism_classificationAmino Acid MetabolismMetabolism030104 developmental biologychemistrylcsh:QMorphological DifferentiationTrpM
researchProduct

Physiological and genomic characterisation of Saccharomyces cerevisiae hybrids with improved fermentation performance and mannoprotein release capaci…

2015

Yeast mannoproteins contribute to several aspects of wine quality by protecting wine against protein haze, reducing astringency, retaining aroma compounds and stimulating lactic-acid bacteria growth. The selection of a yeast strain that simultaneously overproduces mannoproteins and presents good fermentative characteristics is a difficult task. In this work, a Saccharomyces cerevisiae × S. cerevisiae hybrid bearing the two oenologically relevant features was constructed. According to the genomic characterisation of the hybrids, different copy numbers of some genes probably related with these physiological features were detected. The hybrid shared not only a similar copy number of genes SPR1…

Saccharomyces cerevisiae ProteinsBiotecnología AgropecuariaSaccharomyces cerevisiaeGene DosageWineSaccharomyces cerevisiaeSPORE TO SPORE MATINGAliments MicrobiologiaMicrobiologyCell WallFermentacióHybridYEAST HYBRIDIZATIONMembrane Glycoproteinsbiologybusiness.industryGeneral MedicineHibridacióbiology.organism_classificationBiotechnologyYeast in winemakingCIENCIAS AGRÍCOLASRARE MATINGFermentationWINE YEASTBiotecnología Agrícola y Biotecnología AlimentariaHybridization GeneticFermentationChristian ministryGenome FungalbusinessFood ScienceInternational journal of food microbiology
researchProduct

Approccio micromorfologico alla sistematica del genere Isoëtes (Isoëtaceae, Lycopodiophyta): analisi della superficie delle megaspore

2011

Settore BIO/01 - Botanica GeneraleSettore BIO/02 - Botanica SistematicaIsoetes morfologia spore SEM sistematicaSettore BIO/03 - Botanica Ambientale E Applicata
researchProduct

Metodologie Integrate per l'indagine aerobilogica in ambienti confinati e semiconfinati di interesse culturale

2012

INTRODUZIONE – Il presente studio è rivolto alla caratterizzazione del bioaerosol in ambienti confinati/semi-confinati che, oltre a fare parte essi stessi del patrimonio culturale, sono spesso preposti alla salvaguardia di manufatti d’interesse storico-artistico, come musei, archivi, biblioteche. Casi particolari sono rappresentati da ipogei e insediamenti rupestri caratterizzati da specifici parametri ambientali (UR, temperatura, illuminamento) che permettono l’istaurarsi e la proliferazione di complesse biocenosi. Infine, essendo gli ambienti utilizzati e fruiti da operatori culturali e visitatori, è necessario valutare il potenziale rischio sanitario connesso con la presenza di particell…

Settore BIO/03 - Botanica Ambientale E Applicatabatteri spore fungine monitoraggio indoor
researchProduct

Resistance to space simulating conditions and sporicidal treatments of spores from bacilli of extreme environments origins: implication for Astrobiol…

Microorganisms able to tolerate environmental extremes, or extremophiles, are ideal candidates to extend our knowledge on the limitations for terrestrial life, including sporicidal treatments, and on their ability to survive under conditions mimicking space environments. The spore resistance of bacilli isolated from extreme environments, cold (Antarctic soils) and hot (shallow hydrothermal vents of Eolian Islands, Italy), was evaluated towards environmental stressors (wet- and dry-heat, low and high pH values), sporicidal treatments and stresses simulating space-environments (UV-C and X-rays irradiations, desiccations by low pressure, exposition to oxidizing agents and low pressure plasma),…

Settore BIO/07 - EcologiaBacillus Spores Astrobiology Extremophiles Radiation Resistance
researchProduct

Field distribution ofSorosphaera viticolain commercial vineyards in Germany

2006

In the year 2000, resting spores of a previously undescribed plasmodiophorid were found in roots of Vitis spp. This plasmodiophorid was identified as a member of the genus Sorosphaera Schroeter and described as Sorosphaera viticola Kirchmair, Neuhauser, Huber. To attain information on the field distribution of Sorosphaera viticola, a selective screening was conducted in two commercial vineyards in Germany. A study to determine a correlation of Sorosphaeraviticola infection to grapevine growth was also performed.

Sorosphaera viticolaGenusPlasmodiophoridBotanyGeneticsMyxomycetesVitisSorosphaeraReproduction sporesBiologyMolecular BiologyMicrobiologySporeFEMS Microbiology Letters
researchProduct

Study of Bacillus subtilis spore's : characterication of stuctures implied in its resistance

2013

The bacterial spore is a multilayer microbial form which is extremely resistant to environmental perturbations. This resistance is especially due to its unique structure which is particularly compact and weakly permeable. This work aims to identify and characterize the spore structures involved in these properties. Overall investigation methods, such as NMR and fluorescence anisotropy, have shown that the cortex of Bacillus subtilis spores is modified by temperature for level similar to that of the activation of germination. This will result in changes to the access to the inner membrane. A tool at the spore’s scale, the fluorescence lifetime imaging microscopy (FLIM) in conjunction with th…

Spores de Bacillus subtilisEthanolMembrane interneCortexInner membraneBacillus subtilis sporesImagerie en temps de vie de fluorescence[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyFluorescence lifetime imaging[SDV.BIO] Life Sciences [q-bio]/Biotechnology
researchProduct

Playing with Time in Digital Fiction

2015

The exceptional quality of digital fictions lies in their inherently dynamic nature, how they may be flexibly programmed to generate new content and alter the already existing contents. This adds a new temporal level, compared to traditional fictions. Digital games, especially, incorporate aspects of simulation and narration in their structure. As interactive and dynamic media form, games are specifically temporal in nature. They offer us the flexibility and preciseness of digital simulations, with the potential of psychologically engaging narrative qualities, which together open up a whole new field of experimenting with temporally dynamic media. Much of the new media fictions partake in a…

Structure (mathematical logic)Flexibility (engineering)Cognitive scienceLiteraturetemporaalisuusbusiness.industryField (Bourdieu)media_common.quotation_subjectthe Spore (game)ComputingMilieux_PERSONALCOMPUTINGkerrontaTemporalityArtNew mediathe Braid (game)WrightQuality (philosophy)fictional timeNarrativesimulointibusinessdigital fictionmedia_common
researchProduct

Indicateurs pour l'évaluation de l'impact de produits phytosanitaires sur la composante microbienne de la qualité biologique des sols

2011

National audience

[SDE] Environmental SciencesCYCLE DU SOUFRECYCLE DE L'AZOTENOMBRE DE COPIE DE GENES FONCTIONNELS[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering[SDV]Life Sciences [q-bio]DEGRADATION DES PESTICIDESBIOINDICATEURSACTIVITES ENZYMATIQUES[SDV.IDA] Life Sciences [q-bio]/Food engineeringSTRUCTURE DE COMMUNAUTES MICROBIENNESABONDANCE DE COMMUNAUTES MICROBIENNES[SDV] Life Sciences [q-bio]POUVOIR DE COLONISATION DE CHAMPIGNONS MA[SDV.IDA]Life Sciences [q-bio]/Food engineering[SDE]Environmental SciencesDIVERSITE BIOCHIMIQUE[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringTEST DE GERMINATION DE SPORESComputingMilieux_MISCELLANEOUS
researchProduct

Spoilage of oat bran by sporogenic microorganisms revived from soil buried 4000 years ago in Iranian archaeological site

2015

Abstract The Bronze Age archaeological site of Shahr-i Sokhta (30° 39′ N; 61° 24’ E), located today in southeastern Iran, Sistan region, is a special archaeological deposit in which the exceptional preservation of human, plant and animal remains, due to the dry climate of the region, can provide detailed information on one of the first complex proto-urban societies. In recent years, there has been growing interest in changes in local climate and environment as major reasons why the settlement was abandoned about 4000 years ago. Food shortage has been regarded as a direct effect of these changes. No attention has been paid to the potential health hazards associated with ancient urban/domesti…

biologyMicroorganismFood spoilageFungifood and beveragesBacillusSporeAspergillus flavusOat branbiology.organism_classificationSettore BIO/19 - Microbiologia GeneralePalynologyArchaeologyMicrobiologyArchaeobotany Bacillus sp. Fungi Oat bran Palynology Phylogenetic tree SporeSporeBiomaterialsPenicilliumBotanyBacillus sp.ArchaeobotanyEnergy sourceWaste Management and DisposalCladosporiumPhylogenetic tree
researchProduct