Search results for " stem"
showing 10 items of 2170 documents
Small extracellular vesicles from young mice prevent frailty, improve healthspan and decrease epigenetic age in old mice
2021
SummaryAging is associated with an increased risk of frailty, disability, comorbidities, institutionalization, falls, fractures, hospitalization, and mortality. Searching for strategies to delay the degenerative changes associated with aging and frailty is interesting. We treated old animals intravenously with small extracellular vesicles (sEVs) derived from adipose mesenchymal stem cells (ADSCs) of young animals, and we found an improvement of several functional parameters usually altered with aging, such as motor coordination, grip strength, fatigue resistance, fur regeneration, and renal function. Frailty index analysis showed that 40% of old control mice were frail, whereas none of the …
Influence of retinol on human chondrocytes in agarose culture
1992
Vitamin A and its congeners, collectively called retinoids, are known to have teratogenic potential and have induced craniofacial and limb malformations in numerous animal species. More importantly, retinoids are recognized as teratogenic to fetuses of pregnant women who have taken such preparations for dermatologic disorders. Information gathered from the study of animal models suggests that retinoids interfere with cartilage differentiation. If chondrogenesis in limb development is disturbed it may contribute to limb reductions and malformations. In vitro studies using various animal systems have shown that cartilage matrix macromolecules are altered to resemble those secreted by mesenchy…
Immune response to the 23-valent polysaccharide pneumococcal vaccine after the 7-valent conjugate vaccine in allogeneic stem cell transplant recipien…
2009
The current recommendations for active immunization after stem cell transplant (SCT) include 3 doses of 7-valent pneumococcal conjugate vaccine (PCV7) from 3 months after transplant, followed by a 23-valent polysaccharide pneumococcal vaccine (PPV23). However, until now, the immune response to PPV23 after PCV7 has not been assessed after SCT. In the EBMT IDWP01 trial, 101 patients received 1 dose of PPV23 at 12 or 18 months, both after 3 doses of PCV7. The efficacy of PPV23 was assessed 1 month later and at 24 months after transplant by the pneumococcal serotype 1 and 5 antibody levels. Serotype 1 and 5 are not included in PCV7. Although the geometric mean concentrations were significantly …
Possible regulatory mechanisms responsible for the high expression of serpin protease inhibitor PI-9 in ER+ -derived breast cancer stem cells.
2015
Breast cancer (BC) is the most common endocrine cancer and the second leading cause of cancer-related death in women. About 75% of BCs expresses high levels of estrogen receptors that sustain the tumor growth. Moreover, in BC estrogens prevent apoptosis induced by granzyme B released by cytotoxic T lymphocytes and NK cells through the production of the granzyme B inhibitor PI-9. As a consequence, cancer cells acquire the ability to escape immune surveillance’s signaling. Although some studies explored the role of PI-9 in BC cells, its presence has not been investigated in cancer stem cells so far. In this research, tertiary tumorspheres were obtained from estrogen receptor-alfa positive (ER…
Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels
2019
Abstract Background Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development. GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG). Methods Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM fo…
OPLA scaffold, collagen I, and horse serum induce a higher degree of myogenic differentiation of adult rat cardiac stem cells
2009
In the last few years, a major goal of cardiac research has been to drive stem cell differentiation to replace damaged myocardium. Several research groups have attempted to differentiate potential cardiac stem cells (CSCs) using bi- or three-dimensional systems supplemented with growth factors or molecules acting as differentiating substances. We hypothesize that these systems failed to induce a complete differentiation because they lacked an architectural space. In the present study, we isolated a pool of small proliferating and fibroblast-like cells from adult rat myocardium. The phenotype of these cells was assessed and the characterized cells were cultured in a collagen I/OPLA scaffold …
Carbohydrate and nutritional responses to stem girdling and drought stress with respect to understanding symptoms of Huanglongbing (HLB) in Citrus
2013
The most important worldwide problem in citrus production is the bacterial disease Huanglongbing (HLB; citrus greening) caused by phloem-limited bacterium Candidatus Liberibacter asiaticus. The earliest visible symptoms of HLB on leaves are vein yellowing and an asymmetrical chlorosis referred to as “blotchy mottle”, thought to be the result of starch accumulation. We tested the hypothesis that such visible symptoms are not unique to HLB by stem girdling 2-yr-old seedlings of two citrus rootstocks with and without drought stress in the greenhouse. After 31 d, girdling had little effect on shoot growth but girdling increased the relative growth rate of shoots in drought stressed trees. Starc…
GROWTH AND BIOMASS PARTITIONING OF YOUNG LOQUAT PLANTS UNDER WATER DEFICIT
2015
Water scarcity in the Mediterranean areas is very common and understanding responses to drought is important for loquat management and production. The objective of this study was to evaluate the effect of water deficit on growth and biomass partitioning of loquat. Ninety 1-year-old plants of 'Marchetto' loquat (Eriobotrya japonica Lindl.) grafted on quince were grown in the greenhouse in 12-liter pots and three water regimes were imposed starting on 11 May and until 27 July, 2013. One-third of the plants was irrigated to reintegrate 100% of the water consumed (CTR); a second group of plants was irrigated with 66% of the water consumed by CTR plants (MD); a third group was irrigated with 33%…
Agro-hydrological models and field measurements to assess the water status of a citrus orchard irrigated with micro-sprinkler and subsurface drip sys…
2021
Compared to the micro-sprinkler irrigation, traditionally used in citrus orchards, subsurface drip systems (SDS) allow increasing the water use efficiency (WUE); when coupled with water-saving strategies, like regulated deficit irrigation (RDI), further increase of WUE are possible. Combining measurements of soil water content (SWC) and weather data with measurements of midday stem water potential (MSWP) makes it possible to identify irrigation scheduling parameters for the RDI. However, measurements of MSWP are destructive and time-consuming, and also require skilled operators. For all these reasons, the use of the agro-hydrological models, such as the FAO-56 model, can be considered a sur…
Relationship between tree water status and physiology, yield, and fruit quality in the table olive (Olea europaea sativa L.) cultivar ‘Nocellara del …
2012
Although the olive (Olea europaea sativa l.) is considered a xerophytic species, long periods of water stress and high temperatures during summer months can greatly affect plant physiology and productivity. In recent years, the use of plant-based water status indicators have become popular in the study of plant-water relationships and in the design of irrigation programs, particularly midday stem water potential (ψstem). The current trend in the irrigation of olive trees is in the development of Deficit Irrigation (DI) systems whereby the water is applied at a rate which is lower than evapotranspiration needs, resulting in only very small reductions in yield. Rather than working towards min…