Search results for " stem"

Plant-based water stress sensing in two olive cultivars with different physiological responses to drought

2022

A large portion of modern olive plantings is dominated by one main cultivar (‘Arbequina’) planted at super high density (SHD, 1,500 tree ha‑1), leading to a dangerous reduction in olive biodiversity. Very few cultivars have growth and productive patterns that suit the SHD system requirements. Partial intensification and mechanization with several cultivars may represent the safest and easiest alternative. The efficient use of limited natural resources, such as water, in SHD systems, requires precise management. Continuous plant-based sensing is a promising approach to managing water, mainly tested on the cultivar ‘Arbequina’. In this study, we evaluated the sensitivity of two promising indi…

FIRST REPORT OF STEMPHYLIUM sp. ON OLIVE TREES IN SICILY.

2011

In the last five years, we have observed a new decline of Olea europaea in north-western and southern Sicily (insular Italy). The syndrome consists in more or less extensive leaf chlorosis, sometimes with irregular necrotic marginal or apical spots. Apical defoliation of twigs showing cortical necrosis and withering has often been observed. Isolations, carried out in 2007/08, yilded a complex of fungal genera associated with the syndrome. Among these, Stemphylium sp., a well-known foliar pathogen of other plants, was constantly isolated from all the alterations observed. In December 2010, single spore colonies of this fungus were used for artificial inoculations on healthy 3-year-old olive …

H2O2 INDUCES NECROPTOSIS IN MESOANGIOBLAST STEM CELLS

2018

Stem cells are used in regenerative medicine, but their therapeutic efficacy is compromised by their huge death during the first days post-transplantation. Indeed, the microenvironment within damaged tissues is hostile for stem cell survival mainly due to oxidative stress. H2O2 may play a relevant role in inducing death of the injected cells. The aim of our study was to determine the mechanism of mesoangioblast (A6) cell death after an H2O2 treatment. FACS analysis with annV/PI showed that H2O2 induced a dose and time-dependent decrement in A6 viability. We have also found an increase in caspases 8, 9 and 3 activity after the treatment. To assess their involvement in cell death, the pan cas…

Effect of conditioned media from osteo- and adipodifferentiating mesenchymal stem cells on triple negative MDA-MB231 breast cancer cells

2013

Essential Oil Composition of Stems and Fruits of Caralluma europaea N.E.Br. (Apocynaceae)

2010

The essential oil of the stems and fruits of Caralluma europaea (Guss.) N.E.Br. (Apocynaceae) from Lampedusa Island has been obtained by hydrodistillation and its composition analyzed. The analyses allowed the identification and quantification of 74 volatile compounds, of which 16 were aromatic and 58 non-aromatic. Stems and fruits contained 1.4% and 2.7% of aromatic compounds respectively, while non-aromatic were 88.3% and 88.8%. Non-aromatic hydrocarbons were the most abundant compounds in both organs, followed by fatty acids. Data showed differences in the profiles between stems and fruits which shared only eighteen compounds; stems accounted for 38 compounds while fruits for 53. Fruits …

STEM CELLS IN BREAST CANCER DEVELOPMENT AND MALIGNANT PROGRESSION

Perinatal and Wharton's jelly-derived mesenchymal stem cells in cartilage regenerative medicine and tissue engineering strategies

2011

Stem cells can be found in embryonic and extraembryonic tissues as well as in adult organs. In particular, research in the last few years has delineated the key features of perinatal stem cells derived from fetus-associated tissues. These cells show multiple differentiation potential, can be easily expanded ex vivo, and raise no ethical concerns as regards their use. Several reports indicate that cells isolated from Wharton's jelly (WJ), the main component of umbilical cord extracellular matrix, are multipotent stem cells that express markers shared by other mesenchymal stem cells (MSC) and give rise to different mature cell types belonging to all three germ layers. Moreover, WJ-MSC display…

Wharton's Jelly Mesenchymal Stem Cells and Immune Modulation: Regenerative Medicine Meets Tissue Repair

2013

Umbilical cord revisited: From Wharton's jelly myofibroblasts to mesenchymal stem cells

2013

The umbilical cord (UC) is an essential part of the placenta, contributing to foetal development by ensuring the blood flow between mother and foetus. The UC is formed within the first weeks of gestation by the enclosure of the vessels (one vein and two arteries) into a bulk of mucous connective tissue, named Wharton's jelly (WJ) and lined by the umbilical epithelium. Since their first identification, cells populating WJ were described as unusual fibroblasts (or myofibroblasts). Recent literature data further highlighted the functional interconnection between UC and the resident cells. The UC represents a reservoir of progenitor populations which are collectively grouped into MSCs (mesenchy…

CARDIAC STEM CELLS AND BIOMATERIALS: INDUCTION OF MYOGENIC DIFFERENTIATION AND IMPLANTION OF BIOSYNTHETIC AND NATURAL MATRICES IN THE ADULT HEART

2014

Principal purpose of regenerative medicine for the cardiac tissue is to find the best way to inoculate stem cells in a specific myocardial area damage, improving their homing, integration and survival. To achieve this goal, the field of biomaterials is important to bypass this obstacles, modulating the environment for implanted cells and enhance CSC function in the heart. Biomaterials can mimic or include naturally occurring extracellular matrix and instruct stem cell function in different ways: promoting angiogenesis, enhancing stem cell engraftment and differentiation, and accelerating electromechanical integration of transplanted cells. The aim of this thesis was to assess whether the pr…