Search results for " stem"
showing 10 items of 2170 documents
Isolation and phenotypical characterization of mesenchymal stem cells from the Wharton's jelly of pre-term human umbilical cord
2014
Wharton's jelly mesenchymal stem cells immunomodulatory molecules: their journey from umbilical cord to differentieted cells.
2014
Wharton's jelly mesencymal stem cells differentiated into hepatocyte-like cells show expression of immunomodulatory molecules
2014
Characterization of the immunomodulatory properties of mesenchymal stem cells isolated from Wharton's jelly
2012
Wharton’s jelly mesenchymal stem cells differrentiation towards hepatocyte-like cells: in vitro evidences
2012
Extending the usefulness of extraembryonic mesenchymal stem cellsin regenerative medicine: expression of novel markers, immunoregulatory molecules, a…
2010
Mesenchymal stem cells (MSC) are considered promising tools in regenerative medicine applications. Even if they are already applied clinically, their extended characterization is being increasingly viewed as a needed feature, in order to avoid contrasting results when translating “in vitro” experiments to “in vivo” approaches. We recently demonstrated in human MSC isolated from the umbilical cord matrix (HEMSC) the expression of novel markers indicative of their stemness, as well as differentiative and immune properties [1]. HEMSC were cultured and subject to multiple molecular and morphological analyses to determine the expression of markers of interest. Undifferentiated HEMSC expressed im…
Extended characterization of hypoimmunogenic mesenchymal stem cells isolated from the subendocardial layer of human hearts from chronic heart failure…
2009
Purpose: Mesenchymal stem cells (MSC) are pluripotent cells which are known to reside in a number of adult organs. In human heart, various populations of stem/progenitor cells have been isolated by different groups. Several efforts still need to be made to better characterize resident or migrating MSC populations in terms of markers expression and immunogenic potential prior to their use for regenerative medicine applications in heart diseases. Methods: MSC were isolated from the sub-endocardial layer of left ventricle sections of hearts explanted from patients affected by chronic heart failure (CHF). The expression of several markers characteristic of the MSC lineage was assessed at both t…
Isolation and characterization of pluripotent cells from the subendocardial layer of human hearts from chronic heart failure.
2008
Purpose: Several literature reports indicated the existence of pluripotent cells residing into the heart wall, even if in vivo heart regeneration is still a poorly comprised phenomenon. Post-infarct chronic heart failure is a pathology for which heart transplant remains a key option of clinical intervention. The amount of data collected in recent years forces researchers to better characterize the properties of adult stem cells, especially for those isolated from heart. Methods: We developed a new protocol to obtain, from the subendocardial layer of post-infarct hearts explanted from chronic heart failure patients undergoing heart transplantation, populations of mesenchymal stem cells. The …
Isolation, characterization, differentiative properties of human mesenchymal stem cells isolated from the sub-endocardial layer of post-infarct chron…
2009
Mesenchymal stem cells (MSC) are pluripotent cells which reside in several adult organs, including heart, even if heart regeneration in vivo is still a poorly comprised phenomenon. Contrasting literature reports suggest that several efforts should be made to better characterize resident or migrating MSC populations (for both markers expression and immunogenic potential) prior to their effective use for regenerative medicine applications in heart diseases. We developed a new protocol to obtain human sub-endocardial MSC (HSE-MSC) from post-infarct hearts explanted from chronic heart failure (CHF) patients undergoing heart transplantation. We characterized HSE-MSC by immunocytochemistry (ICC) …