Search results for " stem"
showing 10 items of 2170 documents
Spheroids of adipose derived stem cells show their potential in differentiating towards the angiogenic lineage
2023
Introduction: Adipose derived stem cells (ASCs) are a mesenchymal stem cell population of great scientific interest due to their abundance and easiness in obtaining them from adipose tissue. Recently, several techniques for three dimensional (3D) ASCs cultivation have been developed to obtain spheroids of adipose stem cells (SASCs). It was already proved that ASCs are able to differentiate towards the endothelial lineage thus, for the first time, we investigated the ability of our 3D SASCs to differentiate endothelially and the effects of not differentiated SASC secreted factors on specific cultured cells. Materials and methods: SASCs were differentiated with a specific medium towards endot…
The Crosstalk of Adipose-Derived Stem Cells (ADSC), Oxidative Stress, and Inflammation in Protective and Adaptive Responses
2020
International audience; The potential use of stem cell-based therapies for the repair and regeneration of various tissues and organs is a major goal in repair medicine. Stem cells are classified by their potential to differentiate into functional cells. Compared with other sources, adipose-derived stem cells (ADSCs) have the advantage of being abundant and easy to obtain. ADSCs are considered to be tools for replacing, repairing, and regenerating dead or damaged cells. The capacity of ADSCs to maintain their properties depends on the balance of complex signals in their microenvironment. Their properties and the associated outcomes are in part regulated by reactive oxygen species, which medi…
Impact of viable CD45 cells infused on lymphocyte subset recovery after unrelated cord blood transplantation in children
2010
International audience; We studied lymphocyte recovery in 88 children who consecutively underwent unrelated cord blood transplantation for malignant (n = 64) or nonmalignant (n = 24) diseases. All children but 3 received myeloablative conditioning regimens with pretransplant antithymocyte globulin. Median age was 5.6 years (0.1-18 years) and median follow-up was 40 months (10-136 months). The median dose of infused viable CD45(+) cells (vCD45) was 3.35 × 10(7)/kg with a ratio infused vCD45/collected total nucleated cell at 0.46. Immunologic endpoints were: time to achieve CD3(+) >500 and 1500/mm(3), CD4(+) >500/mm(3), CD8(+) >250/mm(3), CD19(+) >200/mm(3), natural killer >100/mm(3). These e…
Terminal tendon cell differentiation requires the glide/gcm complex.
2004
International audience; Locomotion relies on stable attachment of muscle fibres to their target sites, a process that allows for muscle contraction to generate movement. Here, we show that glide/gcm and glide2/gcm2, the fly glial cell determinants, are expressed in a subpopulation of embryonic tendon cells and required for their terminal differentiation. By using loss-of-function approaches, we show that in the absence of both genes, muscle attachment to tendon cells is altered, even though the molecular cascade induced by stripe, the tendon cell determinant, is normal. Moreover, we show that glide/gcm activates a new tendon cell gene independently of stripe. Finally, we show that segment p…
Dual mode of action of grape cane extracts against Botrytis cinerea
2019
International audience; Crude extracts of Vitis vinifera canes represent a natural source of stilbene compounds with well characterized antifungals properties. In our trials, exogenous application of a stilbene extract (SE) obtained from grape canes on grapevine leaves reduces the necrotic lesions caused by Botrytis cinerea. The SE showed to possess a direct antifungal activity by inhibiting the mycelium growth. The activation of some grapevine defense mechanism was also investigated. H2O2 production and activation of mitogen-activated protein kinase (MAPK) phosphorylation cascades as well as accumulation of stilbenoid phytoalexins were explored on grapevine cell suspension. Moreover, the t…
Conceptos básicos sobre la robótica como herramienta pegagógica
2017
Material docente perteneciente al curso del Servei de Formació Permantent de la Universitat de València "Robótica educativa como herramienta pedagógica" que se imparte dentro del programa de Formación del Profesorado No Universitario. Se define la Robótica Educativa como herramienta pedagógica y se introduce y desarrolla dicho concepto dentro del marco de las TIC. A continuación, se presenta el robot a emplear como marco central del material docente, realizando tanto un análisis de los elementos electrónicos y mecánicos que lo conforman, como de las posibles aplicaciones que pueden realizarse con el mismo. Específicamente se lleva a cabo una introducción a la Robótica Educativa y posibles p…
Descripción de NXT Mindstorm: elementos hardware y programación
2017
Material docente perteneciente al curso del Servei de Formació Permantent de la Universitat de València "Robótica educativa como herramienta pedagógica" que se imparte dentro del programa de Formación del Profesorado No Universitario. Este bloque de contenidos describe las características específicas del robot NXT Mindstorm, de modo que se definen sus características funcionales y los elementos concretos que lo conforman. En este sentido se definen los sensores de luz, ultrasonidos, sonido y contacto; así como los actuadores que lo conforman: motores, pantalla y altavoz. Por último, se determina su interconexionado y se definen las características del montaje y arquitectura empleada.
Elementos de un Robot: Entradas - Procesador - Salidas
2017
Material docente perteneciente al curso del Servei de Formació Permantent de la Universitat de València "Robótica educativa como herramienta pedagógica" que se imparte dentro del programa de Formación del Profesorado No Universitario. Este material docente se emplea para presentar los elementos básicos de un sistema robótico, así como la arquitectura de un robot: partes fundamentales. Procesador, sensores y actuadores. En este sentido, se definen los principios de funcionamiento de diferentes sensores a través de su funcionamiento físico (temperatura, distancia, luz, sonido...). Asimismo, se definen diferentes ejemplos de actuadores como LEDs, motores, relés...
Adipose stem cells on the basis of tumor transformation
Mesenchymal stem cells thanks to their differentiated multipotent ability are considered the most promising candidate for tissue engineering and regenerative medicine. The source of mesenchymal stem cells up to about 20 years was represented by the bone marrow, but due to their limited amount of cells together with a very invasive and painfull surgical treatment, poorly accepted by patients, has led the scientific community to investigate an alternative mesenchymal stem cells source with similar properties. In particular, the adipose tissue has attracted the greatest interest thanks to its relative abundance, the easy of availability and the large amounts of mesenchymal stem cells which are…
Adipose-derived stem cells
2013
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