Search results for " tests"
showing 10 items of 3284 documents
Measuring mutagenicity in ecotoxicology: A case study of Cd exposure in Chironomus riparius.
2021
Abstract Existing mutagenicity tests for metazoans lack the direct observation of enhanced germline mutation rates after exposure to anthropogenic substances, therefore being inefficient. Cadmium (Cd) is a metal described as a mutagen in mammalian cells and listed as a group 1 carcinogenic and mutagenic substance. But Cd mutagenesis mechanism is not yet clear. Therefore, in the present study, we propose a method coupling short-term mutation accumulation (MA) lines with subsequent whole genome sequencing (WGS) and a dedicated data analysis pipeline to investigate if chronic Cd exposure on Chironomus riparius can alter the rate at which de novo point mutations appear. Results show that Cd exp…
Development of an in vitro neuroblastoma 3D model and its application for sterigmatocystin-induced cytotoxicity testing
2021
Abstract Given the increasing importance of establishing better risk assessments for mycotoxins, novel in vitro tools for the evaluation of their toxicity are mandatory. In this study, an in vitro 3D spheroid model from SH-SY5Y cells, a human neuroblastoma cell line, was developed, optimized and characterized to test the cytotoxic effects caused by the mycotoxin sterigmatocystin (STE). STE induced a concentration- and time-dependent cell viability decrease in spheroids. Spheroids displayed cell disaggregation after STE exposure, increasing in a dose-dependent manner and over time. STE also induced apoptosis as confirmed by immunofluorescence staining and Western blot. Following the decrease…
Cardiac changes in subclinical and overt hyperthyroid women: retrospective study
2003
This retrospective and descriptive 4-year study was undertaken to describe cardiac changes in subclinical and overt hyperthyroidism.We revised the charts of 386 consecutive cardiopathic women whose blood samples were referred to the Radioimmunoassay Laboratory to evaluate thyroid function from 1 January 1997 through 31 December 2000.After excluding women because euthyroid or hypothyroid, or taking amiodarone and women with hypertension, rheumatic disease, myocardial infarction, a total of 31 hyperthyroid women were thus selected for the study: 19 with subclinical hyperthyroidism and 12 with overt hyperthyroidism. The prevalence of atrial fibrillation did not differ between subclinical (48%)…
Hormonal responsiveness in the Trier Social Stress Test and the dexamethasone‐corticotropin releasing hormone test in healthy individuals
2021
A number of different laboratory procedures investigate the hormonal response in a standardized pharmacological challenge test (dexamethasone-corticotropin releasing hormone; DEX-CRH) or in a psychosocial stress induction on the hypothalamic-pituitary-adrenocortical axis by the Trier Social Stress Test (TSST). However, the magnitude of the response related to the different stressors and the interaction of the responsiveness between the two tests is still unclear. Fifty-two participants underwent both the DEX-CRH test and the TSST on two separate days. The cortisol and the plasma adrenocorticotropic hormone (ACTH) release were assessed before and after the stress tests. For a specification o…
Pituitary resistance to thyroid hormone action with preserved circadian rhythm of thyrotropin in a postmenopausal woman.
1992
We assessed the 24-h behavior of circulating TSH and the dopaminergic control on TSH release in a postmenopausal woman, who had elevated levels of serum thyroid hormones and an inappropriately high concentration of serum TSH, indicating pituitary resistance to thyroid hormone action. The patient was found to have a daily profile of serum TSH similar to that of normal subjects, except for the persistently elevated 24 h levels, suggesting that alterations in thyroid hormone negative feedback control did not affect substantially circadian TSH rhythm. The acute administration of a dopamine antagonist drug (metoclopramide) resulted in a markedly elevated peak of serum TSH, similar both in the mo…
How to predict university performance: a case study of a prestigious Turkish university
2016
International audience; Turkey's education system is based on a centralized selection system, starting from secondary education level. For post-secondary studies, students are sorted according to their scores in a competitive national exam. By statute, Galatasaray University, a French speaking institution founded in 1992 by Turkey and France, enrolls half of its students from the very best candidates while the other half comes from French speaking high schools (relatively top-ranked). These students must be classified amongst the first 25,000 in the national competition to be admitted as well as passing a competitive exam in French, although their national ranking remains lower than the fir…
Novel Perspectives in the Detection of Oral and Nasal Oxidative Stress and Inflammation in Pediatric United Airway Diseases
2010
United airway disease (UAD) concept proposed that asthma and rhinitis are both different clinical manifestation of a single inflammatory process. The aim of this study is to assess in upper and lower airways the level of inflammation and oxidative stress and to investigate the relationship between biomarkers in persistent allergic rhinitis (PER) and in concomitant asthma with PER. By a crosssectional study we measured oral and nasal (FENO) and oral and nasal EBC 8-isoprostane, LTB4 and PGE2 in children with PER (n=14) and with PER and concomitant intermittent asthma (IA; n=25), mild persistent asthma (mA; n=28), moderate persistent asthma (MA; n=13) and in Healthy Controls (HCs; n=13). Oral…
Measuring and Modelling Evaporation Losses from Wet Branches of Lemon Trees
2022
Evaporation losses of rainfall intercepted by canopies depend on many factors, including the temporal scale of observations. At the event scale, interception is a few millimetres, whereas at a larger temporal scale, the number of times that a canopy is filled by rainfall and then depleted can make the interception an important fraction of the rainfall depth. Recently, a simplified interception/evaporation model has been proposed, which considers a modified Merrian model to compute interception during wet spells and a simple power-law equation to model evaporation from wet canopy during dry spells. Modelling evaporation process at the sub hourly temporal scale required the two parameters of …
Some recent results on the compressive and flexural behaviour of composite members
2005
Experiemntal results from compression and flexural tests on steel tubulare columns having circular or rectangular transverse cross-section and infilled with plain or fiber reinforced concrete (FRC) are analysez. for composite members differenti lengths and different dimensions of transverse cross-section were considered in order to assess the influence on behavior in flexure and in compression.The fiber investigated with different percentage were syntetic, carbon and deformed steel fibers. The constituent materials were characterised through compressive and split tests on conconcrete cylinders. Load.shortning and load-delection curves were determined including the sotnening response. Simpli…
Physical Fitness in Young Men between 1975 and 2015 with a Focus on the Years 2005–2015
2018
Purpose: The purpose of the present study was to investigate changes in physical fitness and anthropometry of young men entering the military service in Finland during the years 1975–2015. Methods: The study included the fitness test results of 627,142 healthy young male conscripts (age 19.1 T 0.4 yr). Data included results of aerobic capacity, muscle fitness tests, and anthropometric characteristics. Results: The results show that the increase in mean body mass of young men has slowed down during the last 10 yr. However, the total increase in body mass was 6.8 kg (8.8%, P e 0.001) between 1993 and 2015. The mean distance achieved in the 12-min running test decreased by 337 m (12.2%, P e 0.…