Search results for " three-dimensional"

EXPRESSION AND LOCALIZATION OF “CHAPEROKINE” HSP60 IN BRONCHIAL CULTURE MODELS MIMING COPD

Redrawing the Past, reimaiging the Future. Gamification project for the reinterpretation of historical architecture

2021

The objectives of the work we present concern the reinterpretation of architectural traces of the past with a "gamification" project for educational laboratories to be activated in schools of different order and grade. The methodology used starts from the 3D-reconstruction of disappeared architectures, urban plots where the stratification lends itself to different interpretations, based on the documentary information found and the hypotheses that can be spent directly from reading the artefact. Then it translates everything into diversified outputs to tell the evolution of the monument and the site and its conjectural reconfiguration: from immersive 3D visualization to 3D printing of modula…

La Piazza del Garraffello a Palermo tra iconografia storica e attualità

2018

The proposed contribution is focused on the graphic analysis of an eighteenth-century perspective view portraying one of the most ancient squares of the City of Palermo (piazza Garraffello). It is an engraving print made by F. Cichè in 1711 on commission of the Guild of Silversmiths: the square is completely described with the fountain (still existing) in the foreground, and with the corporate lodges of foreign nations in the background (these two buildings nowadays have disappeared). In the picture is also visible a temporary triumphal arch with canopy erected by the same guild who had commissioned the view: this is the object of main perspectival interest. The study of the perspective use…

Biocompatibility and biodegradability of electrospun phea-pla scaffolds: Our preliminary experience in a murine animal model

2012

We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonizatio…

MultiSlice human organ extraction based on GVF

2008

Segmentation techniques based on active contours algorithm are widely used in medical imaging. Unfortunately, they require a lot of parameters to be used and this can rep- resent an issue for those physicians with not much informatics skills. This paper proposes a software tool which allows to segment multiple slice can differ organ extraction setting a small number of parameters. Moreover, the tool offers the functionality to perform a multiple segmentation the same time, so that an entire volume composed by hundreds slices can be segmented.

FINDUS: An Open-Source 3D Printable Liquid-Handling Workstation for Laboratory Automation in Life Sciences

2020

3D-printed laboratory devices can enable ambitious research purposes even at a low-budget level. To follow this trend, here we describe the construction, calibration, and usage of the FINDUS (Fully Integrable Noncommercial Dispensing Utility System). We report the successful 3D printing and assembly of a liquid-handling workstation for less than $400. Using this setup, we achieve reliable and flexible liquid-dispensing automation with relative pipetting errors of less than 0.3%. We show our system is well suited for several showcase applications from both the biology and chemistry fields. In support of the open-source spirit, we make all 3D models, assembly instructions, and source code ava…

Facial scanning accuracy depending on the alignment algorithm and digitized surface area location: An in vitro study.

2021

To measure the accuracy (trueness and precision) of a facial scanner depending on the alignment method and the digitized surface area location.Fourteen markers were adhered on a head mannequin and digitized using an industrial scanner (GOM Atos Q 3D 12 M; Carl Zeiss Industrielle Messtechnik GmbH). A control mesh was acquired. Subsequently, the mannequin was digitized using a facial scanner (Arc4; Bellus3D) (n = 30). The control mesh was delineated into 10 areas. Based on the alignment procedures, two groups were created: reference best fit (RBF group) and landmark-based best fit (LA group). The root mean square was used to calculate the discrepancy between the control mesh and each facial s…

An Automatic Three-Dimensional Fuzzy Edge Detector

2009

Three-dimensional object analysis is of particular interest in many research fields. In this context, the most common data representation is boundary mesh, namely, 2D surface embedded in 3D space. We will investigate the problem of 3D edge extraction, that is, salient surface regions characterized by high flexure. Our automatic edge detection method assigns a value, proportional to the local bending of the surface, to the elements of the mesh. Moreover, a proper scanning window, centered on each element, is used to discriminate between smooth zones of the surface and its edges. The algorithm does not require input parameters and returns a set of elements that represent the salient features …

Boolean operations with implicit and parametric representation of primitives using R-functions

2005

We present a new and efficient algorithm to accurately polygonize an implicit surface generated by multiple Boolean operations with globally deformed primitives. Our algorithm is special in the sense that it can be applied to objects with both an implicit and a parametric representation, such as superquadrics, supershapes, and Dupin cyclides. The input is a constructive solid geometry tree (CSG tree) that contains the Boolean operations, the parameters of the primitives, and the global deformations. At each node of the CSG tree, the implicit formulations of the subtrees are used to quickly determine the parts to be transmitted to the parent node, while the primitives' parametric definition …

Printing Life-Inspired Subcellular Scale Compartments with Autonomous Molecularly Crowded Confinement.

2019

A simple, rapid, and highly controlled platform to prepare life-inspired subcellular scale compartments by inkjet printing has been developed. These compartments consist of fL-scale aqueous droplets (few µm in diameter) incorporating biologically relevant molecular entities with programmed composition and concentration. These droplets are ink-jetted in nL mineral oil drop arrays allowing for lab-on-chip studies by fluorescence microscopy and fluorescence life time imaging. Once formed, fL-droplets are stable for several hours, thus giving the possibility of readily analyze molecular reactions and their kinetics and to verify molecular behavior and intermolecular interactions. Here, this pla…