Search results for " trip"
showing 10 items of 535 documents
Temple Architecture
2017
Si esamina l'architettura religiosa di età romana imperiale a Sabratha partendo da un'analisi dei singoli edifici templari, con considerazioni di ordine tipologico e cronologico, valutando i riflessi sull'urbanistica della città. The Roman religious architecture of the Imperial period at Sabratha is examined, moving from an analysis of each building, with considerations about the tipology and the chronology of the buildings and evaluating the consequences on the city's urbanism.
Cholesterol-Inulin Conjugates for Efficient SN38 Nuclear Delivery: Nanomedicines for Precision Cancer Therapy
2022
An amphiphilic inulin-thiocholesterol conjugate (INU-Cys-TC) was strategically designed as a biodegradable core-shell nanocarrier of 7-ethyl-10-hydroxy-camptothecin (SN38) to enhance its solubility and stability in aqueous media, thus exploiting its brilliant anticancer effect. INU-Cys-TC was designed to have the hydrophilic inulin backbone (external shell) partially functionalized with hydrophobic thiocholesterol moieties (internal core) through a biodegradable disulfide bond due to cysteamine bridges. Thiocholesterol moieties impair redox-sensitive self-assembling abilities, yielding to nano-sized micelles in aqueous media capable of efficiently encapsulating a high amount of SN38 (DL = 8…
Role of glycine-82 as a pivot point during the transition from the inactive to the active form of the yeast Ras2 protein
1991
AbstractRas proteins bind either GDP or GTP with high affinity. However, only the GTP-bound form of the yeast Ras2 protein is able to stimulate adenylyl cyclase. To identify amino acid residues that play a role in the conversion from the GDP-bound to the GTP-bound state of Ras proteins, we have searched for single amino acid substitutions that selectively affected the binding of one of the two nucleotides. We have found that the replacement of glycine-82 of the Ras2 protein by serine resulted in an increased rate of dissociation of Gpp(NH)p, a nonhydrolysable analog of GTP, while the GDP dissociation rate was not significantly modified. Glycine-82 resides in a region that is highly conserve…
A short-range gradient of histone H3 acetylation and Tup1p redistribution at the promoter of the Saccharomyces cerevisiae SUC2 gene.
2003
Chromatin immunoprecipitation assays are used to map H3 and H4 acetylation over the promoter nucleosomes and the coding region of the Saccharomyces cerevisiae SUC2 gene, under repressed and derepressed conditions, using wild type and mutant strains. In wild type cells, a high level of H3 acetylation at the distal end of the promoter drops sharply toward the proximal nucleosome that covers the TATA box, a gradient that become even steeper on derepression. In contrast, substantial H4 acetylation shows no such gradient and extends into the coding region. Overall levels of both H3 and H4 acetylation rise on derepression. Mutation of GCN5 or SNF2 lead to substantially reduced SUC2 expression; in…
Specific Defects in Different Transcription Complexes Compensate for the Requirement of the Negative Cofactor 2 Repressor in Saccharomyces cerevisiae
2007
Abstract Negative cofactor 2 (NC2) has been described as an essential and evolutionarily conserved transcriptional repressor, although in vitro and in vivo experiments suggest that it can function as both a positive and a negative effector of transcription. NC2 operates by interacting with the core promoter and components of the basal transcription machinery, like the TATA-binding protein (TBP). In this work, we have isolated mutants that suppress the growth defect caused by the depletion of NC2. We have identified mutations affecting components of three different complexes involved in the control of basal transcription: the mediator, TFIIH, and RNA pol II itself. Mutations in RNA pol II in…
Partial purification and characterization of succinyl-CoA synthetase from Saccharomyces cerevisiae
1983
Succinyl-CoA synthetase from Saccharomyces cerevisiae was partially purified (20-fold) with a yield of 44%. The Michaelis-Menten constants were determined: Km (succinate) = 17 mM; Km (ATP) = 0.13 mM; Km (CoA) = 0.03 mM. The succinyl-CoA synthetase has a molecular weight of about 80000 dalton (as determined by polyacrylamide gradient gel electrophoresis). The pH optimum is at 6.0. During fermentation the activity of succinyl-CoA synthetase is lower than in aerobically grown yeast cells. The presence of succinyl-CoA synthetase in fermenting yeasts may be regarded as an indication for the oxidative formation of succinate. In fermenting yeast cells succinyl-CoA synthetase is repressed by glucos…
Novel Glutamate–Putrescine Ligase Activity in Haloferax mediterranei: A New Function for glnA-2 Gene
2021
This article belongs to the Section Cellular Biochemistry.
Enseñanza de las ciencias : revista de investigación y experiencias didácticas
2021
Se expone el proceso seguido por un equipo de docentes de secundaria con el objetivo de cambiar la metodología de trabajo en el aula mediante el diseño y aplicación de una secuencia de aprendizaje definida a partir del aprendizaje basado en retos que integre de manera interdisciplinar los contenidos de Biología y Geografía en segundo de la ESO. Mediante la reflexión didáctica compartida, organizada y continuada se transforma una salida de campo tradicional en un nuevo proyecto de trabajo que se desarrolla a lo largo de un trimestre completo. Los resultados muestran la nueva secuencia de actividades creada, la percepción de los estudiantes y familias y los beneficios en la implicación del al…
Depletion of cytosolic GSH decreases the ATP levels and viability of synaptosomes from aged mice but not from young mice
1995
The effect of glutathione depletion on the viability of freshly isolated synaptosomes from whole brain was investigated in young and aged mice. Aging did not influence the GSH and ATP levels and the viability of these synaptosomes. However depletion of glutathione caused by the cytosolic glutathione inhibitor diethyl maleate (1 mM) resulted in a significant decline, after 60 min of incubation, in ATP levels and viability in the synaptosomes from aged mice but not in those from young mice. When synaptosomes were incubated in the presence of the mitochondrial glutathione inhibitor ethacrynic acid (0.2 mM) there was a similar decline in glutathione, ATP levels and synaptosomal viability, both …
TRAVELLING TOWARDS AND FROM MINOR ISLANDS THROUGH NON-CONVENTIONAL AIR TRANSPORT: DEMAND AND COST ANALYSIS
2010
This research addresses the role of innovative forms of passenger air transport in favouring mobility of tourists visiting minor islands. In particular, we studied the feasibility of scheduled transport services using helicopter and seaplane for connecting Sicily in the south of Italy, to the near and very attractive Eolie Islands rapidly. In order to estimate the potential service demand, we allowed for the number of tourists arriving in the Eolie Archipelago during the period 1999-2008. In detail, we considered only the market of visitors with a high willingness-to-pay for time savings (individuals choosing superior hotels) coming from origins at least 300 km away from the Eolie Islands. …