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showing 10 items of 1421 documents
Changes in the physiological and agricultural characteristics of peat-based Bradyrhizobium japonicum inoculants after long-term storage
2000
International audience; Commercial soybean inoculants processed with sterilised peat and stored at 20 °C for 1–8 years were used as experimental materials to assess the changes in the physiological activity of Bradyrhizobium japonicum after storage. Viable counts decreased and physiological characteristics of the bacterium changed during storage, with an increase in the time taken for colony appearance on a medium without yeast extract, an increase in the lag time for nodule appearance on soybean grown in glass tubes and a decrease in survival on seeds. All the inoculants produced a significant increase in grain yield in a field experiment. The percentage of efficient cells in the field (re…
The use of soil electrical resistivity to monitor plant and soil water relationships in vineyards
2015
Abstract. Soil water availability deeply affects plant physiology. In viticulture it is considered as a major contributor to the "terroir" expression. The assessment of soil water in field conditions is a difficult task especially over large surfaces. New techniques, are therefore required to better explore variations of soil water content in space and time with low disturbance and with great precision. Electrical Resistivity Tomography (ERT) meets these requirements, for applications in plant sciences, agriculture and ecology. In this paper, possible techniques to develop models that allow the use of ERT to spatialise soil water available to plants are reviewed. An application of soil wate…
Transcription of two blue copper-binding protein isogenes is highly correlated with arbuscular mycorrhizal development in Medicago truncatula.
2010
International audience; Expression profiling of two paralogous arbuscular mycorrhizal (AM)-specific blue copper-binding gene (MtBcp1a and MtBcp1b) isoforms was performed by real-time quantitative polymerase chain reaction in wild-type Medicago truncatula Jemalong 5 (J5) during the mycorrhizal development with Glomus intraradices for up to 7 weeks. Time-course analysis in J5 showed that expression of both MtBcp1 genes increased continuously and correlated strongly with the colonization intensity and arbuscule content. MtPT4, selected as a reference gene of the functional plant-fungus association, showed a weaker correlation to mycorrhizal development. In a second experiment, a range of mycor…
Consequences of gene flow between transgenic, insect-resistant crops and their wild relatives
2015
Concerns have been raised about the possibility of the negative impact of transgenic crops on the environment. If wild plants accidentally pick up transgenes through pollen exchange with genetically modified crops, they could gain new environmental advantages or conversely suffer genetic homogenization and loss of biodiversity. This could cause weeds that might create new problems in farmers' fields and wild habitats and change biological equilibriums. Examples of gene flow are given for several cases of crop/ancestor pairs and two cases of more distantly related species. The main concern is that introgression between transgenic, insect-resistant crops and their wild relatives could lead to…
Do plant-based biogeographical regions shape aphyllophoroid fungal communities in Europe?
2018
Aim: Aphyllophoroid fungi are associated with plants, either using plants as a resource (as parasites or decomposers) or as symbionts (as mycorrhizal partners). In spite of their strong association with plants, it is unknown how much plant distributions determine their biogeographical patterns compared with environmental factors such as climate and human land use. In this study, our aims are to (1) describe the spatial diversity patterns of aphyllophoroid fungi in Europe and (2) identify the factors shaping these patterns. Location: Europe, as well as the adjacent Subarctic to Arctic islands (Greenland, Faroe Islands, Iceland, Svalbard), Palestine and the south-east coast of the Caspian Sea…
Assessment of genetically modified maize Bt11 x MIR162 x 1507 x GA21 and three subcombinations independently of their origin, for food and feed uses …
2018
In this opinion, the GMO Panel assessed the four-event stack maize Btll x MIR162 x 1507 x GA21 and three of its subcombinations, independently of their origin. The GMO Panel previously assessed the four single events and seven of their combinations and did not identify safety concerns. No new data on the single events or the seven subcombinations leading to modification of the original conclusions were identified. Based on the molecular, agronomic, phenotypic and compositional characteristics, the combination of the single events in the four-event stack maize did not give rise to food/feed safety issues. Based on the nutritional assessment of the compositional characteristics of maize Btll …
Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with othe…
2019
Background Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops. In Italy, the first ToBRFV outbreak occurred in 2018 in several provinces of the Sicily region. ToBRFV outbreak represents a serious threat for tomato crops in Italy and the Mediterranean Basin. Methods Molecular and biological characterisation of the Sicilian ToBRFV ToB-SIC01/19 isolate was performed, and a sensitive and specific Real-time RT-PCR TaqMan minor groove binder probe method was developed to detect ToBRFV in infected plants and seeds. Moreover, four different sample preparation procedures (immunocapture, total RNA extractio…
Humusica 1, article 1: Essential bases – Vocabulary
2018
International audience; The Special Issue Humusica 1 corresponds to a field guide for the classification of terrestrial humus systems and forms. The present first article of the issue defines vocabulary, objects and concepts necessary for: (a) field investigation, (b) understanding the process of classification, (c) assigning ecological significance to the defined morpho-functional units, (d) discussing and exchanging scientific data about humus systems. The article starts with general considerations, as the necessity humans have to classify natural objects for sharing ideas and information on them. Then the article focuses on soil as functional element of every ecosystem. Historical and re…
Production of 3-hydroxy-γ-decalactone, the precursor of two decenolides with flavouring properties, by the yeast Yarrowia lipolytica
2009
3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation at low aeration (0.40 molar yields) and, to a lesser extent, at lower pH (0.15). As oxygen played a key-role, we evaluated its effect at fixed dissolved oxygen and at the pH which was the most favourable to the biotransformation (pH 4.5). At 5% and 30% dissolved oxygen, yields reached …
Influence des phosphates naturels et des mycorhizes à vésicules et à arbuscules sur la croissance et la nutrition minérale de Zizyphus mauritiana Lam…
1998
Rock phosphate and vesicular-arbuscular mycorrhiza effects on growth and mineral nutrition of Zizyphus mauritiana Lam. in an alkaline soil. The influence of vesicular-arbuscular mycorrhizae (VAM) and rock phosphate (RP) was studied on jujube (Zizyphus mauritiana Lam.), a multipurpose fruit tree in Sahelian agroforestry systems. Jujubes were inoculated or not by Clomus manihotis Howeler, Sieverding & Schenck in an alkaline sandy soil to which were added five levels of RP (12 % of P) : 0.00, 0.31, 0.62, 1.25 et 2.50 g P/kg of soil, equivalent to 0, 775, 1 550, 3 125 et 6 250 kg P ha -1 . The jujubes seedlings grew poorly without mycorrhizal colonization and without RP applications (figures 1a…