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showing 10 items of 360 documents

Differences in the nitric oxide/soluble guanylyl cyclase signalling pathway in the myocardium of neonatal and adult rats

2000

Abstract The effects of a nitric oxide-donor, S -nitroso- N -acetylpenicillamine, and a direct activator of soluble guanylyl cyclase, 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1), on force of contraction ( F c ) and L-type Ca 2+ currents ( I Ca(L) ) were investigated in myocardial preparations from neonatal and adult rats. Since hearts from adult and neonatal animals contained 160 and 47 mg/100 g wet weight myoglobin, respectively, its possible interaction with both drugs was also investigated. Both S -nitroso- N -acetylpenicillamine (100 μM) and YC-1 (30 μM) were ineffective in myocardial preparations from adult rats but reduced the magnitude of I Ca(L) and F c in preparations fr…

Maleinorganic chemicalsmedicine.medical_specialtyContraction (grammar)Calcium Channels L-Typechemistry.chemical_elementCalciumNitric OxideNitric oxideRats Sprague-Dawleychemistry.chemical_compoundInternal medicinemedicineAnimalsCyclic GMPPharmacologychemistry.chemical_classificationDose-Response Relationship DrugMyoglobinMyocardiumPenicillamineAge FactorsMyocardial ContractionIn vitroRatsEndocrinologyEnzymeAnimals NewbornchemistryMyoglobinGuanylate Cyclasemedicine.symptomSoluble guanylyl cyclaseSignal TransductionMuscle contractionEuropean Journal of Pharmacology
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Role of K+ and Ca2+ fluxes in the cerebroarterial vasoactive effects of sildenafil

2007

The aim of this study was to assess the role of K(+) and Ca(2+) fluxes in the cerebroarterial vasoactive effects of the phosphodiesterase-5 inhibitor sildenafil. We used isolated rabbit basilar arteries to assess the effects of extracellular K(+) raising on sildenafil-induced vasodilatation, and studied the pharmacological interaction of sildenafil with selective modulators of membrane K(+) and Ca(2+) channels. Expression of Kv1 subunits of K(+) channels was assessed at messenger and protein levels. Parallel experiments were carried out with zaprinast for comparison. Sildenafil (10 nM-0.1 mM) induced concentration-dependent relaxation of endothelin-1 (10 nM)-precontracted arteries, which wa…

Malemedicine.medical_specialtyCalcium Channels L-Typemedicine.drug_mechanism_of_actionPhosphodiesterase InhibitorsVasodilationIn Vitro TechniquesPharmacologyPiperazinesSildenafil Citratechemistry.chemical_compoundInternal medicinemedicineAnimalsChannel blockerRNA MessengerSulfonesPharmacologyTetraethylammoniumDose-Response Relationship DrugChemistryDepolarization3-Pyridinecarboxylic acid 14-dihydro-26-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)- Methyl esterIberiotoxinEndocrinologyPurinesBasilar ArterycGMP-specific phosphodiesterase type 5PotassiumShaker Superfamily of Potassium ChannelsCalciumRabbitsZaprinastPhosphodiesterase 5 inhibitorEuropean Journal of Pharmacology
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Long-term serial kinetics of N-terminal pro B-type natriuretic peptide and carbohydrate antigen 125 for mortality risk prediction following acute hea…

2017

Aim: Baseline values of N-terminal pro B-type natriuretic peptide (NT-proBNP) and carbohydrate antigen 125 (CA125) predict all-cause mortality in acute heart failure (AHF). However, there is limited information about the added prognostic benefit of using longitudinal values, and how this predictive ability is modified when modelling together. The aim of this study was to determine the mutually-adjusted association between the longitudinal trajectories of NT-proBNP and CA125 with all-cause mortality after an episode of AHF. Methods and results: We included 946 consecutive patients discharged for AHF. NT-proBNP and CA125 were measured at each physician-patient encounter (median (interquartile…

Malemedicine.medical_specialtyLongitudinal studyTime Factorsacute heart failuremedicine.drug_class030204 cardiovascular system & hematologyCritical Care and Intensive Care MedicineRisk Assessment03 medical and health sciences0302 clinical medicineInterquartile rangeRisk FactorsInternal medicineCause of DeathNatriuretic Peptide BrainmedicineNatriuretic peptideHumans030212 general & internal medicineMortalitycarbohydrate antigen 125Survival rateCause of deathAgedRetrospective StudiesHeart Failurebusiness.industrylongitudinal studyMembrane ProteinsRetrospective cohort studyGeneral MedicineBrain natriuretic peptidemedicine.diseasePrognosisPeptide FragmentsSurvival RateEndocrinologyB-type natriuretic peptideSpainHeart failureCA-125 AntigenAcute DiseaseCardiologyFemaleCardiology and Cardiovascular MedicinebusinessBiomarkersFollow-Up StudiesEuropean heart journal. Acute cardiovascular care
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Comparison of N-terminal proB-type natriuretic peptide levels at different stages of visceral leishmaniosis and in patients with chronic kidney disea…

2018

N-terminal proB-type natriuretic peptide (NT-proBNP) may be a useful marker in canine leishmaniosis (CanL). The aim was to compare NT-proBNP in dogs at different LeishVet stages of CanL and with idiopathic chronic kidney disease (CKD). Dogs diagnosed with CanL or CKD and a group of healthy dogs were included (group A, five normal dogs; group B, six dogs LeishVet 1-2; group C, 13 dogs LeishVet 3-4; group D, six dogs with CKD). NT-proBNP was higher (P0.001) in group C (7.616 pmol/l, interquartile range (IQR) 3537-10,000 pmol/l) than in group A (293 pmol/l, IQR 257-373), group B (388.5 pmol/l, IQR 324-793) and group D (740 pmol/l, IQR 557-962 pmol/l). International Renal Interest Society (IRIS…

Malemedicine.medical_specialtyMyocarditisN-Terminal ProB-type Natriuretic Peptide040301 veterinary sciencesmedicine.drug_classLeft ventricular hypertrophyGastroenterologyGroup A0403 veterinary scienceDogsInternal medicineNatriuretic Peptide BrainmedicineNatriuretic peptideAnimalsHumansIn patientcardiovascular diseasesDog DiseasesRenal Insufficiency ChronicGeneral Veterinarybusiness.industry0402 animal and dairy science04 agricultural and veterinary sciencesGeneral Medicinemedicine.disease040201 dairy & animal sciencePeptide FragmentsLeishmaniasis VisceralFemalebusinesshormones hormone substitutes and hormone antagonistsBiomarkersKidney diseaseThe Veterinary record
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Heart-type fatty acid binding protein is a sensitive biomarker for early AMI detection in troponin negative patients: a pilot study

2017

Background: Early detecting AMI in individuals presenting to the ED with chest pain continues to be a challenge. cTn is the gold standard for AMI diagnosis but early presenters (<1 hours from symptom onset) maybe cTn negative on admission. We analysed the diagnostic value of h-FABP and hs-TnI in patients presenting to ED with chest pain and no cTnI elevations. Methods: 28 AMI and 28 no-AMI individuals both presented to ED within one hour from pain onset were included. Blood donors were analysed for h-FABP cut-off identification. Among AMI patients, 55% were positive for h-FABP and 34.6% were positive for hs-TnI (p = .015), thus 21% were positive only for h-FABP. The diagnostic accuracy w…

Malemedicine.medical_specialtyPathologytroponin-negativehealth care facilities manpower and serviceshsTnIClinical BiochemistryMyocardial InfarctionPilot Projects030204 cardiovascular system & hematologyChest painClinical biochemistryGastroenterologyAMI03 medical and health sciences0302 clinical medicineRisk FactorsInternal medicineHumansMedicinecardiovascular diseases030212 general & internal medicinehealth care economics and organizationsbiologybusiness.industryTroponin IGeneral MedicineGold standard (test)Middle AgedTroponinEDEarly DiagnosisHeart-type fatty acid binding proteinbiology.proteinBiomarker (medicine)Femalemedicine.symptombusinessFatty Acid Binding Protein 3h-FABPBiomarkers
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The predictive value of heart-type fatty acid-binding protein is independent from symptom duration in normotensive patients with pulmonary embolism.

2013

Heart-type fatty acid-binding protein (H-FABP) is a useful biomarker for risk stratification of patients with pulmonary embolism (PE). In patients with acute myocardial infarction, H-FABP plasma concentrations rise after 30 minutes and return to normal within 20-24 hours. We tested whether the predictive value of H-FABP is affected by the duration of symptoms prior to diagnosis in patients with PE.We prospectively studied 257 consecutive normotensive patients with confirmed symptomatic PE.Patients with acute (24 hours; n=150) symptom onset presented more often with syncope (28.7% vs. 6.5%; p0.001) compared to patients with symptoms ≥ 24 hours (n=107); other baseline characteristics, comorbi…

Malemedicine.medical_specialtyRenal function030204 cardiovascular system & hematologyFatty Acid-Binding Proteins03 medical and health sciences0302 clinical medicineInternal medicinemedicineHumans030212 general & internal medicineMyocardial infarctionProspective StudiesAgedbusiness.industryArea under the curveHematologyOdds ratioMiddle Agedmedicine.diseasePrognosisConfidence interval3. Good healthPulmonary embolismHeart-type fatty acid binding proteinCardiologyBiomarker (medicine)lipids (amino acids peptides and proteins)FemalebusinessPulmonary EmbolismFatty Acid Binding Protein 3Thrombosis research
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Thrombolysis with saruplase versus streptokinase in acute myocardial infarction: five-year results of the PRIMI trial.

1999

Abstract Background Short-term safety and efficacy of thrombolysis with saruplase in acute myocardial infarction have been shown in several trials. To assess long-term outcome of patients treated with saruplase or streptokinase for myocardial infarction, a 5-year follow-up of patients included in the Pro-Urokinase in Myocardial Infarction Trial was performed. Methods and Results Follow-up data are available from 8 centers on 255 (92.4%) of 276 included patients. The 5-year mortality rate was comparable with 20.8% of patients in the saruplase group and 16.9% in the streptokinase group (odds ratio 1.29, 95% confidence interval 0.69 to 2.42). In both groups, a considerable number of fatal card…

Malemedicine.medical_specialtyStreptokinasemedicine.medical_treatmentMyocardial InfarctionAnginaCohort StudiesFibrinolytic AgentsRecurrenceAngioplastyInternal medicineMedicineHumansStreptokinaseThrombolytic TherapyMyocardial infarctionAngioplasty Balloon CoronaryCoronary Artery BypassAgedbusiness.industryOdds ratioThrombolysisMiddle Agedmedicine.diseaseSurvival AnalysisUrokinase-Type Plasminogen ActivatorConfidence intervalRecombinant ProteinsCerebrovascular DisordersCardiologySaruplaseFemaleCardiology and Cardiovascular Medicinebusinessmedicine.drugFollow-Up StudiesAmerican heart journal
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Analysis of N-terminal pro-B-type natriuretic peptide in patients with acute coronary syndromes

2009

Background The N-terminal portion of brain natriuretic peptide (NT-proBNP) has been identified as an indicator of prognosis in different cardiovascular diseases. The objective of this study was to determine the utility of measuring plasma NT-proBNP levels in patients with acute coronary syndromes. Methods and results We studied 66 patients admitted in our division for acute coronary syndromes. Patients underwent a venous blood sample within 24 h from the admission to determine NT-proBNP levels. Increasing plasma levels of NT-proBNP (in tertiles) was associated with a greater history of hypertension and current smoking, whereas biochemical parameters were associated with higher level of crea…

Malemedicine.medical_specialtymedicine.drug_classCreatineRisk AssessmentVentricular Dysfunction Leftchemistry.chemical_compoundPredictive Value of TestsRecurrenceNt-proBNP N-terminal pro-B-type natriuretic peptide acute coronary sydromeInternal medicineNatriuretic Peptide BrainmedicineNatriuretic peptideCreatine Kinase MB FormHumansIn patientHospital MortalityAcute Coronary SyndromeAgedInpatientsCreatininebiologybusiness.industryTroponin IGeneral MedicineLength of StayMiddle AgedPrognosisBrain natriuretic peptidePeptide FragmentschemistryCreatininePredictive value of testsCardiologybiology.proteinFemaleCreatine kinaseN terminal pro b type natriuretic peptideCardiology and Cardiovascular MedicinebusinessBiomarkersCoronary Artery Disease
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Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection

2018

The opportunistic fungal pathogen Candida albicans frequently causes diseases such as oropharyngeal candidiasis (OPC) in immunocompromised individuals. Although it is well appreciated that the cytokine IL-17 is crucial for protective immunity against OPC, the cellular source and the regulation of this cytokine during infection are still a matter of debate. Here, we directly visualized IL-17 production in the tongue of experimentally infected mice, thereby demonstrating that this key cytokine is expressed by three complementary subsets of CD90+ leukocytes: RAG-dependent αβ and γδ T cells, as well as RAG-independent ILCs. To determine the regulation of IL-17 production at the onset of OPC, we…

Malemedicine.medical_treatment2405 ParasitologyPathology and Laboratory Medicine10263 Institute of Experimental ImmunologyMonocytesMice0302 clinical medicineAnimal CellsCandida albicansBiology (General)Candida albicansMononuclear Phagocyte SystemFungal PathogensInnate Immune Systemeducation.field_of_studyEukaryotaMononuclear phagocyte systemFlow CytometryCorpus albicans3. Good healthSpectrophotometryMedical MicrobiologyCytokinesCytophotometryCellular Types10244 Institute of VirologyQH301-705.5Immune CellsImmunologyMicrobiology03 medical and health sciences1311 GeneticsGenetics1312 Molecular BiologyeducationMicrobial PathogensMolecular BiologyMouth2403 ImmunologyBlood CellsOrganismsBiology and Life SciencesDendritic CellsMolecular DevelopmentYeastMice Inbred C57BLMannose-Binding Lectins030104 developmental biologyImmunologyThy-1 Antigens570 Life sciences; biologyParasitologyImmunologic diseases. AllergyDigestive SystemDevelopmental Biology0301 basic medicineNeutrophilsPhysiologyInterleukin-1betaYeast and Fungal ModelsInterleukin-23White Blood CellsSpectrum Analysis TechniquesCandidiasis OralImmune PhysiologyLeukocytesMedicine and Health SciencesCandidaStainingbiologyInterleukin-172404 MicrobiologyCell StainingSpecific Pathogen-Free OrganismsInfectious DiseasesCytokineExperimental Organism SystemsAntigens SurfaceFemaleAnatomyPathogensResearch ArticleLangerinPopulationMycologyOpportunistic InfectionsResearch and Analysis MethodsTongueImmunityVirologymedicineAnimalsLectins C-TypeInterleukin 6Interleukin-6Mouth MucosaFungiCell BiologyRC581-607biology.organism_classificationSpecimen Preparation and TreatmentImmune Systembiology.protein2406 VirologySpleen030215 immunology
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A fixed-point problem with mixed-type contractive condition

2020

We consider a fixed-point problem for mappings involving a mixed-type contractive condition in the setting of metric spaces. Precisely, we establish the existence and uniqueness of fixed point using the recent notions of $F$-contraction and $(H,\varphi)$-contraction.

MatematikNumerical AnalysisPure mathematicsApplied MathematicsMixed-type contractive conditionMixed typeFixed pointFixed pointMetric spaceFixed point problemSettore MAT/05 - Analisi MatematicaUniquenessMetric spaceMathematicsAnalysisMathematics
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