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showing 10 items of 360 documents

Peritoneal Cavity is a Route for Gut-Derived Microbial Signals to Promote Autoimmunity in Non-Obese Diabetic Mice

2015

Macrophages play a crucial role in innate immune reactions, and peritoneal macrophages (PMs) guard the sterility of this compartment mainly against microbial threat from the gut. Type 1 diabetes (T1D) is an autoimmune disease in which gut microbiota and gut immune system appear to contribute to disease pathogenesis. We have recently reported elevated free radical production and increased permeability of gut epithelium in non-obese diabetic (NOD) mice. Impaired barrier function could lead to bacterial leakage to the peritoneal cavity. To explore the consequences of impaired gut barrier function on extra-intestinal immune regulation, we characterized peritoneal lavage cells from young newly w…

Antigens Differentiation T-LymphocyteLipopolysaccharidesmedicine.medical_specialtymiceT-LymphocytesT cellBlotting WesternImmunologyWeaningNodBiologyta3111Peritoneal cavityImmune systemSpecies SpecificityAntigens CDMice Inbred NODInternal medicinediabeticmedicineAnimalsLectins C-TypeIntestinal Mucosamicrobial signalsCells CulturedNOD miceMice Inbred BALB CInnate immune systemTumor Necrosis Factor-alphanon-obeseMicrobiotaautoimmunityta1182ta3141General MedicineFlow CytometryGut EpitheliumIntestinesMice Inbred C57BLInterleukin-1 Receptor-Associated KinasesEndocrinologymedicine.anatomical_structureperitoneal cavityImmunologyMacrophages PeritonealTumor necrosis factor alphaInjections IntraperitonealSignal TransductionScandinavian Journal of Immunology
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Mycobacterial antigen(s) induce anergy by altering TCR- and TCR/CD28-induced signalling events: insights into T-cell unresponsiveness in leprosy.

2009

Present study investigates the role of Mycobacterium leprae (M. leprae) antigens on TCR- and TCR/CD28-induced signalling leading to T-cell activation and further correlates these early biochemical events with T-cell anergy, as prevailed in advanced stages of leprosy. We observed that both whole cell lystae (WCL) and soluble fraction of M. leprae sonicate (MLSA) not only inhibited TCR, thapsigargin and ionomycin induced calcium fluxes by diminishing the opening of calcium channels, but also TCR- or TCR/CD28-induced proximal signalling events like phosphorylation of Zap-70 and protein kinase-C (PKC) activity. Study of TCR- and TCR/CD28-induced downstream signals revealed that M. leprae antige…

Antigens Differentiation T-LymphocyteMAP Kinase Signaling SystemT cellT-LymphocytesImmunologyReceptors Antigen T-Cellchemical and pharmacologic phenomenaBiologyLymphocyte ActivationJurkat cellsp38 Mitogen-Activated Protein Kinaseschemistry.chemical_compoundJurkat CellsCD28 AntigensAntigens CDLeprosyCalcium fluxmedicineHumansLectins C-TypeEnzyme InhibitorsPromoter Regions GeneticMolecular BiologyMycobacterium lepraeProtein Kinase CCell ProliferationClonal AnergyAntigens BacterialMitogen-Activated Protein Kinase 3ZAP-70 Protein-Tyrosine KinaseIonophoresNFATC Transcription FactorsIonomycinT-cell receptorInterleukin-2 Receptor alpha SubunitCD28hemic and immune systemsNFATbiology.organism_classificationCell biologyMycobacterium lepraemedicine.anatomical_structurechemistryGene Expression RegulationIonomycinImmunologyInterleukin-2ThapsigarginCalciumMolecular immunology
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Overexpression of genes involved in lymphocyte activation and regulation are associated with reduced CRM-derived cardiac remodelling after STEMI

2021

Abstract Aims Lymphopenia after ST-segment elevation myocardial infarction (STEMI) correlates with deleterious cardiac consequences and worse prognosis. An in-depth examination of genes implicated in lymphocyte proliferation, activation and regulation and their association with short- and long-term cardiac structure and function is therefore of great interest. Methods Peripheral blood mononuclear cells were isolated from 10 control subjects and 64 patients with a first STEMI treated with primary percutaneous coronary intervention and submitted to cardiac magnetic resonance after 1 week and 6 months. mRNA expression of genes implicated in lymphocyte activation (CD25 and CD69) and regulation …

Antigens Differentiation T-LymphocyteMale0301 basic medicinemedicine.medical_specialtyLymphocytemedicine.medical_treatmentProgrammed Cell Death 1 ReceptorImmunologyGene Expressionchemical and pharmacologic phenomenaLymphocyte proliferationLymphocyte Activation03 medical and health sciences0302 clinical medicineAntigens CDInternal medicineHumansImmunology and AllergyMedicineCytotoxic T cellCTLA-4 AntigenLectins C-TypeIL-2 receptorMyocardial infarctionGeneAgedPharmacologyVentricular Remodelingbusiness.industryInterleukin-2 Receptor alpha SubunitPercutaneous coronary interventionHearthemic and immune systemsOdds ratioMiddle Agedmedicine.diseaseMagnetic Resonance ImagingPathophysiology030104 developmental biologymedicine.anatomical_structure030220 oncology & carcinogenesisCancer researchLymphocyte activationLeukocytes MononuclearCardiologyST Elevation Myocardial InfarctionFemaleCardiology and Cardiovascular MedicinebusinessInternational Immunopharmacology
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Defective T cell receptor/CD3 complex signaling in human type I diabetes

1994

The autoimmune process leading to the destruction of pancreatic β-cells is mediated by T lymphocytes. Peripheral T cells from subjects with preclinical and clinical type I diabetes respond weakly in vitro to lectin stimulation. We, therefore, investigated in a group of newly diagnosed diabetic patients the presence of a defect in the signal transduction pathway of the T cell receptor (TcR)/CD3 complex. Following stimulation with anti-CD3-coupled beads, the proliferative response in diabetic T cells was significantly decreased in comparison with that from normal T cells. Interestingly, addition of either recombinant interleukin (IL)-2 or phorbol 12-myristate 13-acetate to the cell culture wa…

Antigens Differentiation T-Lymphocytemedicine.medical_specialtyT-LymphocytesCD3ImmunologyBiologyLymphocyte ActivationInterleukin 21Antigens CDInternal medicinemedicineHumansImmunology and AllergyCytotoxic T cellLectins C-TypeIL-2 receptorProtein Kinase CInterleukin 3ZAP70T-cell receptorCD28Molecular biologyDiabetes Mellitus Type 1EndocrinologyReceptor-CD3 Complex Antigen T-Cellbiology.proteinCalciumEuropean Journal of Immunology
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Opposite vascular activity of (R)-apomorphine and its oxidised derivatives. Endothelium-dependent vasoconstriction induced by the auto-oxidation meta…

2003

We have synthetised a series of oxidised apomorphine derivatives (orto and para quinones 2-5), in order to analyse their vascular activity. We have performed radioligand binding assays on rat cortical membranes and functional studies on rat aortic rings. Instead the relaxant activity exhibited by (R)-apomorphine, o-quinones 2, 4, show contractile activity dependent on endothelium in rat aortic rings. Compound 2, the main metabolite of (R)-apomorphine auto-oxidation, was the product which showed enhanced contractile activity by a complex mechanism related to activation of Ca(2+) channels through release and/or inhibition of endothelial factors. Moreover, this compound disrupts the endothelia…

ApomorphineCalcium Channels L-TypeEndotheliumMetaboliteRadioligand Assaychemistry.chemical_compoundDrug DiscoverymedicineAnimalsVasoconstrictor AgentsEnzyme InhibitorsRats WistarAortaCerebral CortexPharmacologyDose-Response Relationship DrugbiologyOrganic ChemistryQuinonesStereoisomerismGeneral MedicineReceptors Adrenergic alphaReceptors GABA-AAcetylcholineIn vitroRatsApomorphineNitric oxide synthasemedicine.anatomical_structureBiochemistrychemistryVasoconstrictionBiophysicsbiology.proteinFemaleEndothelium VascularNitric Oxide Synthasemedicine.symptomOxidation-ReductionVasoconstrictionAcetylcholineBlood vesselmedicine.drugEuropean Journal of Medicinal Chemistry
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TP53 codon 72 polymorphism and cervical cancer

2009

Background Cervical cancer is caused primarily by human papillomaviruses (HPV). The polymorphism rs1042522 at codon 72 of the TP53 tumour-suppressor gene has been investigated as a genetic cofactor. More than 80 studies were done between 1998 and 2006, after it was initially reported that women who are homozygous for the arginine allele had a risk for cervical cancer seven times higher than women who were heterozygous for the allele. However, results have been inconsistent. Here we analyse pooled data from 49 studies to determine whether there is an association between TP53 codon 72 polymorphism and cervical cancer.Methods Individual data on 7946 cases and 7888 controls from 49 different st…

ArginineMESH : Polymorphism GeneticMESH: Genes p53MESH : AgedPhysiologyUterine Cervical NeoplasmsMESH: Papillomavirus Infections[ SDV.CAN ] Life Sciences [q-bio]/Cancer0302 clinical medicineGenotypeMESH : FemaleCervical cancerGeneticsMESH: AgedMESH : Papillomavirus Infections0303 health sciencesMESH: Middle AgedHPV infectionMESH: Genetic Predisposition to DiseaseMiddle AgedMESH : AdultWILD-TYPE P53Hardy–Weinberg principle3. Good healthMESH: Uterine Cervical NeoplasmsOncologyMESH: Young Adult030220 oncology & carcinogenesisMeta-analysisFemaleAdultAdolescentMESH : Uterine Cervical NeoplasmsMESH : Young Adult[SDV.CAN]Life Sciences [q-bio]/CancerMESH : Genes p5303 medical and health sciencesYoung AdultSQUAMOUS INTRAEPITHELIAL LESIONSMESH : AdolescentINDIAN WOMENMESH: Polymorphism GeneticmedicineHumansGenetic Predisposition to DiseaseMESH : Middle AgedAllele030304 developmental biologyAgedMESH: AdolescentMESH: HumansPolymorphism GeneticHUMAN-PAPILLOMAVIRUS TYPE-16business.industryP53 ARG72PRO POLYMORPHISMHEALTHY WOMENPapillomavirus InfectionsMESH : HumansMESH: AdultOdds ratiomedicine.diseaseGenes p53GENOTYPESHARDY-WEINBERG EQUILIBRIUMRISK-FACTORSMESH : Genetic Predisposition to DiseasebusinessMESH: FemaleHPV INFECTIONLancet Oncology
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XMM observations of NGC 2516 stars

2007

We present the characteristics of the X-ray variability of stars in the cluster NGC 2516 as derived from XMM-Newton/EPIC/pn data. The X-ray variations on short (hours), medium (months), and long (years) time scales have been explored. We detected 303 distinct X-ray sources by analysing six EPIC/pn observations; 194 of them are members of the cluster. Stars of all spectral types, from the early-types to the late-M dwarfs, were detected. Cone search capability for table J/A+A/456/977/table2 (X-ray and optical properties of NGC 2516 members in the XMM-Newton/EPIC/pn observations having more than 25 counts.)

Astrophysics and AstronomyAstrophysics::High Energy Astrophysical PhenomenaPhysicsAstrophysics::Cosmology and Extragalactic AstrophysicsLate type starsX-ray sourcesstellar astronomyOptical astronomyCosmologyOpen star clustersPhotometryobservational astronomyX ray sourcesAstrophysics::Solar and Stellar AstrophysicsWide-band photometryNatural SciencesLate-type starsAstrophysics::Galaxy AstrophysicsInfrared photometryWide band photometry
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Abundance signature of M dwarf stars

2020

Most of our current knowledge on planet formation is still based on the analysis of main-sequence, solar-type stars. Conversely, detailed chemical studies of large samples of M-dwarf planet hosts are still missing. We aim to test whether the correlations between the metallicity, individual chemical abundances, and mass of the star and the presence of different type of planets found for FGK stars still holds for the less massive M dwarf stars. Methods to determine in a consistent way stellar abundances of M dwarfs from high-resolution optical spectra are still missing. The present work is a first attempt to fill this gap. We analyse in a coherent and homogeneous way a large sample of M dwarf…

Astrophysics and AstronomyRadial velocityPhysicsExoplanetsChemical abundancesexoplanet astronomyAstrophysics::Cosmology and Extragalactic AstrophysicsLate type starsstellar astronomyInterdisciplinary Astronomyobservational astronomyM starsMetallicityAstrophysics::Solar and Stellar AstrophysicsAstrophysics::Earth and Planetary AstrophysicsNatural SciencesLate-type starsAstrophysics::Galaxy AstrophysicsSpectroscopy
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X-ray emission in cool main sequence stars

2003

The maximum amplitude A_max_ of spot-induced brightness variations from long-term V-band photometry and the ratio L_X_/L_bol_ between X-ray and bolometric luminosities are suitable indicators of the level of magnetic activity in the photosphere and in the corona of late-type stars, respectively. By using these activity indicators we investigate the dependence of coronal X-ray emission on the level of photospheric starspot activity in a homogeneous sample of low mass main-sequence field and cluster stars of different ages (IC 2602, IC 4665, IC 2391, {alpha} Per, Pleiades and Hyades). Cone search capability for table J/A+A/410/671/table1 (Photometry)

Astrophysics and AstronomyX ray sourcesStellar AstronomyMagnetic fieldsPhysicsLate type starsX-ray sourcesAstrophysical ProcessesNatural SciencesLate-type starscosmology
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The Mouse That Roared: A Superflare from the dMe Flare Star EV Lac Detected by Swift and Konus-Wind

2010

We report on a large stellar flare from the nearby dMe flare star EV Lac observed by the Swift and Konus-Wind satellites and the Liverpool Telescope. It is the first large stellar flare from a dMe flare star to result in a Swift trigger based on its hard X-ray intensity. Its peak f_X from 0.3--100 keV of 5.3x10^-8 erg/cm2/s is nearly 7000 times larger than the star's quiescent coronal flux, and the change in magnitude in the white filter is >4.7. This flare also caused a transient increase in EV Lac's bolometric luminosity (L_bol) during the early stages of the flare, with a peak estimated L_X/L_bol ~3.1. We apply flare loop hydrodynamic modeling to the plasma parameter temporal changes …

Astrophysics::High Energy Astrophysical PhenomenaFOS: Physical sciencesFluxAstrophysics::Cosmology and Extragalactic AstrophysicsAstrophysicsLuminositylaw.inventionSettore FIS/05 - Astronomia E AstrofisicalawIonizationX-raysAstrophysics::Solar and Stellar AstrophysicsSolar and Stellar Astrophysics (astro-ph.SR)Astrophysics::Galaxy AstrophysicsPhysicsFlare starAstronomy and Astrophysicsastrofisica fisica stellare stars: activity stars: coronae stars: flare stars: individual: EV Lac stars: late-type X-rays: starsAstrophysics - Solar and Stellar AstrophysicsSpace and Planetary ScienceMagnitude (astronomy)Plasma parameterStellar PhysicAstrophysics::Earth and Planetary AstrophysicsSuperflareFlare
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