Search results for "11"
showing 10 items of 17291 documents
The Paracentrotus lividus metallothionein gene family: structure and expression.
2014
Metallothioneins are metal binding proteins that play a pivotal role in metal homeostasis and detoxification. Since their initial discovery, they have been extensively studied in a variety of organisms ranging from microbes to plants and animals. Organisms often possess multiple genes encoding metallothionein homologs with distinct properties, such as varying affinities for different metals, and in many cases different functions. Despite the plethora of available studies, very little information is known about sea urchin P. lividus MT (1, 2). We previously identified five Pl-MT embryonic cDNAs and we studied their induction after cadmium treatment (3). Now we studied their expression during…
cis-Regulation and chromatin dynamics of the hbox12 gene during the embryogenesis of Paracentrotus lividus.
2014
The GRN specifying the dorsal-ventral (D-V) axis of the sea urchin embryo is currently under investigation. An early input for D-V polarity is given by a redox gradient probably generated by an asymmetrical distribution of maternal mitochondria (1). Only on the future ventral side, the oxidizing environment induces the expression of the nodal gene, an essential regulator of D-V polarization (2). By contrast, on the future dorsal side, a reducing environment activates the hypoxia inducible factor (HIF-1α) (3). The hbox12 transcription repressor is an early marker of the dorsal side of the embryo, in which it negatively regulates the expression of nodal (4, 5). Interestingly, by in silico ana…
Genome wide mapping of the MBF-1 binding sites during embryogenesis of the sea urchin reveals it is a chromatin organizer.
2015
The Zinc-finger MBF1 factor is a transcription activator involved in the expression of the early histone genes during development of the sea urchin embryo (1). The DNA-binding domain of MBF1 shares high sequence similarity with that of the CTCF chromatin organizer but, unexpectedly, extensive in silico analysis failed to identify the sea urchin CTCF ortholog (2, 3). This led us to speculate that MBF1 could have co-opted the function of CTCF during evolution of the echinoderms. To support this hypothesis, we performed the genome-wide MBF1-binding sites mapping in the P. lividus genome, by chromatin immunoprecipitation coupled to next generation sequencing (ChIP-Seq). We observed that MBF1 bi…
The echinoid hbox12 multigene family: copy number variation and spatiotemporal expression
2016
Chromatin dynamics during sea urchin embryogenesis: effects on the neural alpha tubulin PlTa2 gene expression
2011
Expression of PlTa2 gene during sea urchin P. lividus development, is spatially confined to the neural territory and temporally activated from the blastula stage. To evaluate a possible involvement of chromatin modifications in regulation of PlTa2 gene expression we first searched for DNaseI hypersentive sites. We found four sites localized in the introns of the gene, when we used chromatin extracted from embryos at gastrula stage but not from morula stage. This result suggests a possible functional role of the introns in the activation of the expression of PlTa2 gene. Moreover, we used specific antibodies for RNA polymerase II and for different modified form of lysine 9, lysine 27 and lysi…
Isolation and characterization of sea urchin P. lividus microbiota from coelomic fluid
2015
Integrated interpretation of geophysical surveys aimed at tridimensional modeling of a coastal aquifer subjected to sea-water intrusion
2011
Inclusive Decision-making and Campaign: The Participation of Second Generation Migrants for Preventing Radicalization
2019
This paper deals with the analysis of the first results of Oltre project (ISF - DG Migration and Home Affairs, EU) funding for preventing the radicalization of the second-generation of migrants. The non-standard field research was intertwined with online social network profiles investigation. We present the off-line research results. Starting from the different dimensions of the risk of radicalization proposed by the kaleidoscopic overview of risk factors (Sieckelinck and Gielen 2018: 5; Ranstorp 2016), we created a topic guide for the indepth qualitative interviews collecting 42 interviews of 2G youths (18-30 years) in 7 Italian towns, interviewing also several privileged testimonies. Then…
Legacy Effects of Contrasting Long-Term Integrated Weed Management Systems
2022
To reduce reliance on herbicides and maintain crop productivity, integrated weed management (IWM) seeks to optimize synergies between diverse sets of weed management practices combined at the cropping system scale. Nevertheless, data on weed community response to the long-term implementation of IWM practices remain scare. Here, we assessed the effects of four IWM systems with contrasting objectives and practices (S2: transition from superficial tillage to conservation agriculture; S3: no-mechanical weeding; S4: mixed mechanical and chemical weeding; S5: herbicide-free; all with 6 year rotations) compared to a conventional reference (S1: herbicide-based with systematic plowing and a 3 year r…