Search results for "16S"
showing 10 items of 464 documents
Isolation, characterization and determination of biotechnological potential of oil-degrading bacteria from Algerian centre coast
2019
Aims The Algerian coastline is exposed to several types of pollution, including hydrocarbons. The aim of this work was to isolate oil-degrading bacteria and to explore the intrinsic bioremediation potential of part of its contaminated harbour. Methods and results A collection of 119 strains, capable to grow on mineral medium supplemented with hydrocarbons, were obtained from polluted sediment and seawater collected from Sidi Fredj harbour (Algiers). Twenty-three strains were selected for further studies. Sequencing of the 16S rRNA gene showed that most isolates belong to genera of hydrocarbonoclastic bacteria (Alcanivorax), generalist hydrocarbons degraders (Marinobacter, Pseudomonas, Gordo…
Isolation of Gram-positive n-alkane degraders from a hydrocarbon-contaminated Mediterranean shoreline.
2007
Aims: To investigate the petroleum hydrocarbon (HC)-degrading potential of indigenous micro-organisms in a sandy Mediterranean coast, accidentally contaminated with petroleum-derived HCs. Methods and Results: Using culturable methods, a population of Gram-positive n-alkane degraders was detected in the contaminated soil. Five isolates, identified as one Nocardia, two Rhodococcus and two Gordonia strains, were able to degrade medium- and long-chain n-alkanes up to C36 as assessed by growth assays and gas chromatography-mass spectrometry analysis. Diverging alkane hydroxylase-encoding genes (alkB) were detected by PCR, using degenerated primers, in all the strains; multiple sequences were obt…
Molecular phylogeny of the spider family Sparassidae with focus on the genus Eusparassus and notes on the RTA-clade and ‘Laterigradae’
2013
The phylogeny of the spider family Sparassidae is comprehensively investigated using four molecular markers (mitochondrial COI and 16S; nuclear H3 and 28S). Sparassidae was recovered as monophyletic and as most basal group within the RTA-clade. The higher-level clade Dionycha was not but monophyly of RTA-clade was supported. No affiliation of Sparassidae to other members of the 'Laterigradae' (Philodromidae, Selenopidae and Thomisidae) was observed, and the crab-like posture of this group assumed a result of convergent evolution. Only Philodromidae and Selenopidae were found members of a supported clade, but together with Salticidae and Corinnidae, while Thomisidae was nested within the hig…
The influence of backslopping on lactic acid bacteria diversity in tarhana fermentation
2020
Tarhana is produced at batch systems in which the microbiota has changed accordingly to the microbial load from ingredients. In order to stabilize the microbiota, the effects of backslopping carried out under different temperature regimes (25 and 30 °C), pH (3.70 and 4.00) and inoculation rates (5, 10 and 15%) on lactic acid bacteria (LAB) diversity were determined in tarhana dough. LAB and Total Aerobic Mesophilic Bacteria (TAMB) numbers increased in all tarhana dough samples subjected to backslopping. Temperature and pH significantly affected the microbiological diversity of tarhana whereas the different inoculation rates did not. Tarhana dough showed complex tarhana microbiota following …
Variability of the human gut microbiota in space, time, and associated with the irritable bowel syndrome
2013
Esta tesis doctoral se centra en el estudio de una de las comunidades microbianas más complejas y de mayor interés biomédico: la microbiota asociada al intestino distal de humanos. El objetivo principal ha sido estudiar las alteraciones potenciales de la microbiota intestinal en relación con el síndrome del intestino irritable (SII), el desorden funcional del tracto gastrointestinal más común en sociedades occidentales. Nuestra aproximación forma parte de una visión actual sobre las relaciones entre microorganismos y hospedadores que trasciende la clásica y estrecha mirada sobre patógenos concretos y enfermedades infecciosas y la desplaza hacia una concepción más de conjunto, que valora los…
Analysis of the endometrial microbiome and its impact on human reproduction
2021
La implantación es un proceso complejo que requiere la sincronización entre un endometrio receptivo y un blastocisto en desarrollo. En los últimos años, gracias a los avances en las técnicas de secuenciación, se han identificado microorganismos en el útero y se ha visto que pueden tener un efecto sobre los resultados reproductivos. Por ello, el estudio del microbioma endometrial está ganando cada vez más interés en las clínicas de reproducción asistida. En este estudio, planteamos la hipótesis de que la presencia de patógenos bacterianos en el útero tiene consecuencias negativas en la salud reproductiva. El objetivo principal fue caracterizar en profundidad el microbioma endometrial y su im…
Extremophilic microbial communities on photovoltaic panel surfaces: a two‐year study
2020
Solar panel surfaces can be colonized by microorganisms adapted to desiccation, temperature fluctuations and solar radiation. Although the taxonomic and functional composition of these communities has been studied, the microbial colonization process remains unclear. In the present work, we have monitored this microbial colonization process during 24 months by performing weekly measurements of the photovoltaic efficiency, carrying out 16S rRNA gene high‐throughput sequencing, and studying the effect of antimicrobial compounds on the composition of the microbial biocenosis. This is the first time a long‐term study of the colonization process of solar panels has been performed, and our results…
First record of Hemidiaptomus (Gigantodiaptomus) superbus (Schmeil, 1895) in Italy, with notes on distribution and conservation status (Copepoda, Cal…
2011
Hemidiaptomus (Gigantodiaptomus) superbus (Schmeil, 1895) was found in a temporary pool in the Pineta di Classe coastal pine-wood (Ravenna, northern Italy). This is the first official record of a species belonging to the subgenus Gigantodiaptomus of Italian fauna, and it widens the known distribution of this rare diaptomid to the Mediterranean basin. In order to supply data on this rare and poorly known species, original drawings for the Italian population have been provided, and some overlooked morphological features have been highlighted. In light of the growing evidence of the existence of a number of cryptic species in the Diaptomidae family, and considering the variability observed in …
Baseline Gut Microbiota Composition Is Associated With Schistosoma mansoni Infection Burden in Rodent Models
2020
In spite of growing evidence supporting the occurrence of complex interactions between Schistosoma and gut bacteria in mice and humans, no data is yet available on whether worm-mediated changes in microbiota composition are dependent on the baseline gut microbial profile of the vertebrate host. In addition, the impact of such changes on the susceptibility to, and pathophysiology of, schistosomiasis remains largely unexplored. In this study, mice colonized with gut microbial populations from a human donor (HMA mice), as well as microbiota-wild type (WT) animals, were infected with Schistosoma mansoni, and alterations of their gut microbial profiles at 50 days post-infection were compared to …
Solving a Bloody Mess: B-Vitamin Independent Metabolic Convergence among Gammaproteobacterial Obligate Endosymbionts from Blood-Feeding Arthropods an…
2015
Endosymbiosis is a common phenomenon in nature, especially between bacteria and insects, whose typically unbalanced diets are usually complemented by their obligate endosymbionts. While much interest and focus has been directed toward phloem-feeders like aphids and mealybugs, blood-feeders such as the Lone star tick (Amblyomma americanum), Glossina flies, and the human body louse (Pediculus humanus corporis) depend on obligate endosymbionts which complement their B-vitamin-deficient diets, and thus are required for growth and survival. Glossiphoniid leeches have also been found to harbor distinct endosymbionts housed in specialized organs. Here, we present the genome of the bacterial endosy…