Search results for "16SrDNA"
showing 3 items of 3 documents
Direct seeding mulch-based cropping increases both the activity and the abundance of denitrifier communities in a tropical soil
2009
International audience; This study evaluated the impact of direct seeding mulch-based cropping (DMC), as an alternative to conventional tilling (CT), on a functional community involved in N cycling and emission of greenhouse gas nitrous oxide (N2O). The study was carried out for annual soybean/rice crop rotation in the Highlands of Madagascar. The differences between the two soil management strategies (direct seeding with mulched crop residues versus tillage without incorporation of crop residues) were studied along a fertilization gradient (no fertilizer, organic fertilizer, organic plus mineral fertilizers). The activity and size of the denitrifier community were determined by denitrifica…
Diversity of rhizobia nodulating wild shrubs of Sicily and some neighbouring islands
2008
Abstract Legume shrubs have great potential for rehabilitation of semi-arid degraded soils in Mediterranean ecosystems as they establish mutualistic symbiosis with Nfxing rhizobia. Eighty-eight symbiotic rhizobia were isolated from seven wild legume shrubs native of Sicily (Southern Italy) and grouped in operational taxonomic units (OTU) by analysis of the ribosomal internal transcribed spacer (ITS) polymorphism. Partial sequencing of 16S rRNA gene of representative isolates of each OTU revealed that most Genisteae symbionts are related to Bradyrhizobium canariense, B. japonicum and B. elkanii. Teline monspessulana was the only Genistea nodulated by Mesorhizobium strains, and Anagyris foeti…
Characterization of rhizobia nodulating chickpea in Tunisia
2001
Cent cinquante rhizobia nodulant le pois chiche (Cicer arietinum L.) ont ete isoles a partir de sols echantillonnes dans differentes regions de Tunisie. L'inoculation de la plante hote avec ces isolats montre une variabilite dans le temps d'apparition des premieres nodosites. Cinq isolats induisent des nodosites deux semaines apres inoculation alors que pour les 145 isolats restants les nodosites ne sont observees qu'apres au moins quatre semaines. L'etude par PCR/RFLP de l'ADNr 16S a permis de rattacher les isolats du premier groupe a l'espece Mesorhizobium mediterraneum et ceux du second groupe a l'espece Sinorhizobium medicae. La position taxonomique des isolats a ete confirmee par leurs…